A novel kanamycin/G418 resistance marker for direct selection of transformants in Escherichia coli and different yeast species

Yeast

Volumn 27, Issue 4, 2010, Pages 189-195

  Agaphonov, Michael ^a   Romanova, Nina ^a,c   Choi, Eui Sung ^b   Ter Avanesyan, Michael ^a  

^a INSTITUTE OF EXPERIMENTAL CARDIOLOGY   (Russian Federation)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c EMORY UNIVERSITY   (United States)

Author keywords

Antibiotic resistance; Cloning vector; Gene disruption; Transformation; Yeast

Indexed keywords

FUNGAL PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; KANAMYCIN; MAL1 PROTEIN; UNCLASSIFIED DRUG; URA3 PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CLONING VECTOR; CONTROLLED STUDY; EPISOME; ESCHERICHIA COLI; GENE DISRUPTION; GENE EXPRESSION REGULATION; MARKER GENE; NONHUMAN; PICHIA ANGUSTA; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; YEAST;

ESCHERICHIA COLI; FUNGAL PROTEINS; GENETIC MARKERS; GENTAMICINS; KANAMYCIN; TRANSFORMATION, GENETIC; YEASTS;

ESCHERICHIA COLI; PICHIA ANGUSTA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 77952119378     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1741     Document Type: Article

References (19)

1. Bogdanova AI, Agaphonov MO, Ter-Avanesyan MD. 1995. Plasmid reorganization during integrative transformation in Hansenula polymorpha. Yeast 11: 343-353.
2. Chechenova MB, Romanova NV, Deev AV, et al. 2004. C-terminal truncation of α-COP affects functioning of secretory organelles and calcium homeostasis in Hansenula polymorpha. Eukaryot Cell 3: 52-60.
3. Cregg JM, Barringer KJ, Hessler AY, Madden KR. 1985. Pichia pastoris as a host system for transformations. Mol Cell Biol 5: 3376-3385.
4. Cregg JM, Russell KA. 1998. Transformation. In Pichia Protocols, Higgins DR, Cregg JM (eds). Humana: Totowa, NJ; 27-39.
5. Davies J, Jimenez A. 1980. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg 29: 1089-1092.
6. Gatignol A, Baron M, Tiraby G. 1987. Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae. Mol Gen Genet 207: 342-348.
7. Gonzalez C, Perdomo G, Tejera P, et al. 1999. One-step, PCR-mediated, gene disruption in the yeast Hansenula polymorpha. Yeast 15: 1323-1329.
8. Gritz L, Davies J. 1983. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188.
9. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96: 23-28.
10. Ito H, Fukuda Y, Murata K, Kimura A. 1983. Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163-168.
11. Liiv L, Parn P, Alamae T. 2001. Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Gene 265: 77-85.
12. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
13. Sohn JH, Choi ES, Kang HA, et al. 1999. A dominant selection system designed for copy number-controlled gene integration in Hansenula polymorpha DL-1. Appl Microbiol Biotechnol 51: 800-807.
14. Sohn JH, Choi ES, Kim CH, et al. 1996. A novel autonomously replicating sequence (ARS) for multiple integration in the yeast Hansenula polymorpha DL-1. J Bacteriol 178: 4420-4428.
15. +] in the yeast Saccharomyces cerevisiae. Genetics 137: 671-676.
16. Testori A, Listowsky I, Sollitti P. 1994. Direct cloning of unmodified PCR products by exploiting an engineered restriction site. Gene 143: 151-152.
17. Wach A. 1996. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12: 259-265.
18. Wendland J. 2003. PCR-based methods facilitate targeted gene manipulations and cloning procedures. Curr Genet 44: 115-123.
19. Woodcock DM, Crowther PJ, Doherty J, et al. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res 17: 3469-3478.