Effects of different cryoprotective agents on ram sperm morphology and DNAintegrity

Theriogenology

Volumn 73, Issue 9, 2010, Pages 1267-1275

  Nur, Z ^a   Zik, B ^a   Ustuner, B ^a   Sagirkaya, H ^a   Ozguden, C G ^a  

^a ULUDAG UNIVERSITY   (Turkey)

Author keywords

Cryopreservation; Cryoprotective agent; DNA integrity; Ram semen

Indexed keywords

ACRIDINE ORANGE; CRYOPROTECTIVE AGENT; DNA; FLUORESCENT DYE; GLYCEROL; PROPYLENE GLYCOL; SUCROSE; TREHALOSE;

ACROSOME; ANIMAL; ANIMAL DISEASE; APOPTOSIS; ARTICLE; COMPARATIVE STUDY; CRYOPRESERVATION; DNA DAMAGE; DRUG EFFECT; MALE; METHODOLOGY; NICK END LABELING; PHYSIOLOGY; SHEEP; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON MOTILITY; ULTRASTRUCTURE;

ACRIDINE ORANGE; ACROSOME; ANIMALS; APOPTOSIS; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DNA; DNA DAMAGE; FLUORESCENT DYES; GLYCEROL; IN SITU NICK-END LABELING; MALE; PROPYLENE GLYCOL; SEMEN PRESERVATION; SHEEP; SPERM MOTILITY; SPERMATOZOA; SUCROSE; TREHALOSE;

PISUM SATIVUM;

EID: 77952101204     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2009.12.007     Document Type: Article

References (43)

1. Pérez-Pé R., Grasa P., Fernandez-Juan M., Peleato M.L., Cebrián-Pérez J.A., and Muiño-Blanco T. Seminal plasma proteins reduce protein tyrosine phosphorylation in the plasma membrane of cold-shocked ram spermatozoa. Mol Reprod Dev 61 (2002) 226-233
2. Schiller J., Arnhold J., Glander H.J., and Arnold K. Lipid analysis of human spermatozoa and seminal plasma by MALDI-TOF mass spectrometry and NMR spectroscopy-effects of freezing and thawing. Chem Phys Lipids 106 (2000) 145-156
3. Gravance C.G., Vishwanath R., Pitt C., Garner D.L., and Casey P.J. Effects of cryopreservation on bull sperm head morphometry. J Androl 19 (1998) 704-709
4. Glander H.J., and Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 5 (1999) 109-115
5. Paasch U., Sharma R.K., Gupta K.A., Grunewald S., Mascha E.J., Thomas A.J., Glander H.J., and Agarwal A. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod 71 (2004) 1828-1837
6. Host E., Lindenberg S., Kahn J.A., and Christensen F. DNA strand breaks in human sperm cells: a comparison between men with normal and oligozoospermic sperm samples. Acta Obstet Gynecol Scand 78 (1999) 336-339
7. Soylu M.K., Nur Z., Ustuner B., Dogan I., Sagirkaya H., Gunay U., and Ak K. Effects of various cryoprotective agents and extender osmolality on post-thaw ram semen. Bull Vet Inst Pulawy 51 (2007) 241-246
8. Woelders H., Matthijs A., and Engel B. Effects of trehalose sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35 (1997) 93-105
9. Yildiz C., Kaya A., Aksoy M., and Tekeli T. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54 (2000) 579-585
10. Hammadeh M.E., Askari A., Gerorg T., Rosenbaum P., and Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl 22 (1999) 155-162
11. Henry M.A., Noiles E.E., Gao D., Mazur P., and Critser J.K. Cryopreservation of human spermatozoa. IV. The effects of cooling rate and warming rate on the maintenance of motility, plasma membrane integrity, and mitochondrial function. Fertil Steril 60 (1993) 911-918
12. Maxwell W.M.C., Landers A.J., and Evans G. Survival and fertility of ram spermatozoa frozen in pellets, straws and minitubes. Theriogenology 43 (1995) 1201-1210
13. Pontbriand D., Howard J.G., Schieve M.C., Stuart L.D., and Wildt D.E. Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa. Cryobiology 26 (1989) 341-354
14. Sanches-Partida L.G., Maxwell W.M.C., Pateg L.G., and Setchell B.P. Proline and glycine betaine in the cryoprotective diluents for ram spermatozoa. Reprod Fertil Dev 4 (1992) 113-118
15. Stanic P., Tandara M., Sonicki Z., Simunic V., Radakovic B., and Suchanek E. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gynecol Reprod Biol 91 (2000) 65-70
16. Morris I.D., Ilott S., Dixon L., and Brison D.R. The spectrum of DNA damage in human sperm assessed by single cell electrophoresis (COMET assay) and its relationship to fertilization and embryo development. Hum Reprod 17 (2002) 990-998
17. Martin G., Sabido O., Durand P., and Levy R. Cryopreservation induces an apoptosis-like mechanism in bull sperm. Biol Reprod 71 (2004) 28-37
18. Kasimanickam R., Pelzer K.D., Kasimanickam V., Swecker W.S., and Thatcher C.D. Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. Theriogenology 65 (2006) 1407-1421
19. Amman R.P., and Hammerstedt R.H. In vitro evaluation of semen quality: an opinion. J Androl 14 (1993) 397-406
20. Christensen P., Brockhoff P.B., and Lehn-Jensen H. The relationship between semen quality and the non-return rate of bulls. Reprod Dom Anim 34 (1999) 503-507
21. Martí E., Pérez-Pé R., Muiño-Blanco T., and Cebrián-Pérez J.A. Comparative study of four different sperm washing methods using apoptotic markers in ram spermatozoa. J Androl 27 (2006) 746-753
22. Larson K.L., DeJonge C.J., Barnes A.M., Jost L.K., and Evenson D.P. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod 15 (2000) 1717-1722
23. Ahmadi A., and Ng S.C. Fertilizing ability of DNA-damaged spermatozoa. J Exp Zool 284 (1999) 696-704
24. Barroso G., Morshedi M., and Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 15 (2000) 1338-1344
25. Martí E., Pérez-Pé R., Coĺas C., Muĩno-Blanco T., and Cebrían-Ṕerez J.A. Study of apoptosis-related markers in ram spermatozoa. Anim Reprod Sci 106 (2008) 113-132
26. Varisli O., Uguz C., Agca C., and Agca Y. Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Anim Reprod Sci 110 (2008) 256-268
27. Kawakami E., Morita Y., Hori T., and Tsutsui T. Lectin-binding characteristics and capacitation of canine epididymal spermatozoa. J Vet Med Sci 64 (2002) 543-549
28. Abdelhakeam A.A., Graham E.F., and Vazquez I.A. Studies on the absence of glycerol in unfrozen and frozen ram semen: Fertility trials and the effect of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology 28 (1991) 36-42
29. Abdelhakeam A.A., Graham E.F., Vazquez I.A., and Chaloner K.M. Studies on the absence of glycerol in unfrozen and frozen ram semen. Development of an extender for freezing: effects of osmotic pressure, egg yolk levels, type of sugars and the method of dilution. Cryobiology 28 (1991) 43-49
30. Fiser P.S., Ainsworth L., and Fairfull R.W. Evaluation of new diluent and different processing procedures for cryopreservarion of ram semen. Theriogenology 28 (1987) 599-607
31. Gunay U., Dogan I., Nur Z., Manolov I., Sagi{dotless}rkaya H., Soylu M.K., Kaptan C., and Akpi{dotless}nar L. Influence of bull seminal plasma on post-thaw ram semen parameters and fertility. Bull Vet Inst Pulawy 50 (2006) 503-507
32. Hammadeh M.E., Dehn C.H., Hi{dotless}ppach M., Zegi{dotless}ni{dotless}adou T., Sti{dotless}eber M., Georg T., Rosenbaum P., and Schmi{dotless}dt W. Comparison between computerized slow-stage and static liquid nitrogen vapour freezing methods with respect to the deleterious effect on chromatin and morphology of spermatozoa from fertile and subfertile men. Int J Androl 24 (2001) 66-72
33. Salamon S., and Maxwell W.M.C. Frozen storage of ram semen. II. Causes of low fertility after cervical insemination and methods of improvement. Anim Reprod Sci 38 (1993) 1-36
34. Aboagla E.M., and Terada T. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod 69 (2003) 1245-1250
35. An T.Z., Iwakiri M., Edashige K., Sakurai T., and Kasai M. Factors affecting the survival of frozen-thawed mouse spermatozoa. Cryobiology 40 (2000) 237-249
36. Neild D.M., Gadella B.M., Chaves M.G., Miragaya M.H., Colenbrander B., and Aguero A. Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology 59 (2003) 1693-1705
37. Luo X., Budihardjo I., Zou H., Slaughter C., and Wang X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94 (1998) 481-490
38. Tawa P., Hell K., Giroux A., Grimm E., Han Y., Nicholson D.W., and Xanthoudakis S. Catalytic activity of caspase-3 is required for its degradation: stabilization of the active complex by synthetic inhibitors. Cell Death Differ 11 (2004) 439-447
39. Pérez L.J., Valcarcel A., De las Heras M.A., Moses D., and Baldassarre H. Evidence that frozen/thawed ram spermatozoa show accelerated capacitation in vitro as assessed by chlortetracycline assay. Theriogenology 46 (1996) 131-140
40. Benchaib M., Braun V., Lornage J., Hadj S., Salle B., Lejeune H., and Guerin J.F. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 18 (2003) 1023-1028
41. Chan P.J., Corselli J.U., Patton W.C., Jacobson J.D., Chan S.R., and King A. A simple comet assay for archived sperm correlates DNA fragmentation to reduced hyperactivation and penetration of zona-free hamster oocytes. Fertil Steril 75 (2001) 186-192
42. Sun J.G., Jurisicova A., and Casper R.F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod 56 (1997) 602-607
43. Lopes S., Jurisicova A., Sun J., and Casper R.F. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 13 (1998) 896-900