메뉴 건너뛰기




Volumn 11, Issue , 2010, Pages

Selection of optimal reference genes for normalization in quantitative RT-PCR

Author keywords

[No Author keywords available]

Indexed keywords

EXPRESSION LEVELS; MULTIPLE REFERENCES; NEGATIVE CORRELATION; NORMALIZATION STRATEGIES; QUANTITATIVE RT-PCR; ROBUST APPROACHES; SIMULATION STUDIES; VARIANCE ESTIMATE;

EID: 77952074665     PISSN: None     EISSN: 14712105     Source Type: Journal    
DOI: 10.1186/1471-2105-11-253     Document Type: Article
Times cited : (90)

References (15)
  • 1
    • 33644582447 scopus 로고    scopus 로고
    • Quantification of Nucleic Acids by PCR
    • La Jolla: International University Line, Bustin SA
    • Bustin SA. Quantification of Nucleic Acids by PCR. A-Z of Quantitative PCR 2004, 5-46. La Jolla: International University Line, Bustin SA.
    • (2004) A-Z of Quantitative PCR , pp. 5-46
    • Bustin, S.A.1
  • 2
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalisation; strategies and considerations
    • 10.1038/sj.gene.6364190, 15592422
    • Huggett JF, Dheda K, Bustin SA, Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes and Immunity 2005, 6:1-6. 10.1038/sj.gene.6364190, 15592422.
    • (2005) Genes and Immunity , vol.6 , pp. 1-6
    • Huggett, J.F.1    Dheda, K.2    Bustin, S.A.3    Zumla, A.4
  • 3
    • 24944534978 scopus 로고    scopus 로고
    • Real-time PCR for mRNA quantification
    • 10.2144/05391RV01, 16060372
    • Wong ML, Medrano JF. Real-time PCR for mRNA quantification. BioTechniques 2005, 39:75-85. 10.2144/05391RV01, 16060372.
    • (2005) BioTechniques , vol.39 , pp. 75-85
    • Wong, M.L.1    Medrano, J.F.2
  • 4
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002, 3(7):research0034.1-0034.11.
    • (2002) Genome Biology , vol.3 , Issue.7
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 5
    • 0034694128 scopus 로고    scopus 로고
    • Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    • 10.1016/S0165-022X(00)00129-9, 11086195
    • Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 2000, 46:69-81. 10.1016/S0165-022X(00)00129-9, 11086195.
    • (2000) J Biochem Biophys Methods , vol.46 , pp. 69-81
    • Schmittgen, T.D.1    Zakrajsek, B.A.2
  • 6
    • 0346024131 scopus 로고    scopus 로고
    • Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR
    • 88, 90-91
    • Aerts JL, Gonzales MI, Topalian SL. Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR. Biotechniques 2004, 36:84-86. 88, 90-91.
    • (2004) Biotechniques , vol.36 , pp. 84-86
    • Aerts, J.L.1    Gonzales, M.I.2    Topalian, S.L.3
  • 7
    • 3142615272 scopus 로고    scopus 로고
    • Validation of housekeeping genes for normalizing RNA expression in real-time PCR
    • 116, 118-119
    • Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 2004, 37:112-114. 116, 118-119.
    • (2004) Biotechniques , vol.37 , pp. 112-114
    • Dheda, K.1    Huggett, J.F.2    Bustin, S.A.3    Johnson, M.A.4    Rook, G.5    Zumla, A.6
  • 8
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
    • 10.1158/0008-5472.CAN-04-0496, 15289330
    • Andersen CL, Jensen JL, Ørntoft TF. Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets. Cancer Research 2004, 64(15):5245-5250. 10.1158/0008-5472.CAN-04-0496, 15289330.
    • (2004) Cancer Research , vol.64 , Issue.15 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Ørntoft, T.F.3
  • 10
    • 18444417714 scopus 로고    scopus 로고
    • Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data
    • 10.2144/05385MT01, 15945375
    • Abruzzo LV, Lee KY, Fuller A, Silverman A, Keating MJ, Medeiros LJ, Coombes KR. Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data. BioTechniques 2005, 38:785-792. 10.2144/05385MT01, 15945375.
    • (2005) BioTechniques , vol.38 , pp. 785-792
    • Abruzzo, L.V.1    Lee, K.Y.2    Fuller, A.3    Silverman, A.4    Keating, M.J.5    Medeiros, L.J.6    Coombes, K.R.7
  • 12
    • 29344460863 scopus 로고    scopus 로고
    • Statistical modeling in case-control real-time rt-pcr assays, for identification of differentially expressed genes in schizophrenia
    • 10.1093/biostatistics/kxi045, 16049138
    • Sundberg R, Castensson A, Jazin E. Statistical modeling in case-control real-time rt-pcr assays, for identification of differentially expressed genes in schizophrenia. Biostatistics 2006, 7:130-144. 10.1093/biostatistics/kxi045, 16049138.
    • (2006) Biostatistics , vol.7 , pp. 130-144
    • Sundberg, R.1    Castensson, A.2    Jazin, E.3
  • 13
    • 33748070507 scopus 로고    scopus 로고
    • A validated quantitative assay to detect occult micrometastases by RT-PCR of Guanylyl Cyclase C in patients with colorectal cancer
    • 10.1158/1078-0432.CCR-06-0865, 16899600
    • Schulz S, Hyslop T, Haaf J, Bonaccorso C, Nielsen C, Witek ME, Birbe R, Palazzo J, Weinberg D, Waldman SA. A validated quantitative assay to detect occult micrometastases by RT-PCR of Guanylyl Cyclase C in patients with colorectal cancer. Clin Cancer Res 2006, 12(15):4545-4552. 10.1158/1078-0432.CCR-06-0865, 16899600.
    • (2006) Clin Cancer Res , vol.12 , Issue.15 , pp. 4545-4552
    • Schulz, S.1    Hyslop, T.2    Haaf, J.3    Bonaccorso, C.4    Nielsen, C.5    Witek, M.E.6    Birbe, R.7    Palazzo, J.8    Weinberg, D.9    Waldman, S.A.10
  • 15
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • 10.1093/nar/29.9.e45, 55695, 11328886
    • Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 2001, 29:e45. 10.1093/nar/29.9.e45, 55695, 11328886.
    • (2001) Nucleic Acids Research , vol.29
    • Pfaffl, M.W.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.