The pyruvic acid analog 3-bromopyruvate interferes with the tetrazolium reagent MTS in the evaluation of cytotoxicity

Assay and Drug Development Technologies

Volumn 8, Issue 2, 2010, Pages 258-262

  Ganapathy Kanniappan, Shanmugasundaram ^a   Geschwind, Jean Francois H ^a   Kunjithapatham, Rani ^a   Buijs, Manon ^a   Syed, Labiq H ^a   Rao, Pramod P ^a   Ota, Shinichi ^a   Vali, Mustafa ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 BROMOPYRUVIC ACID DERIVATIVE; LIPOCORTIN 5; PYRUVIC ACID DERIVATIVE; TETRAZOLIUM; TRYPAN BLUE; UNCLASSIFIED DRUG;

ARTICLE; BIOLUMINESCENCE; CANCER CELL CULTURE; CELL ADHESION; CELL DEATH; CELL LINE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; DOSE RESPONSE; FALSE POSITIVE RESULT; HUMAN; HUMAN CELL;

ADENOSINE TRIPHOSPHATE; ANIMALS; ANNEXIN A5; BIOLOGICAL ASSAY; CELL LINE; CELL LINE, TUMOR; CELL SURVIVAL; COLORING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG SCREENING ASSAYS, ANTITUMOR; HUMANS; INDICATORS AND REAGENTS; PYRUVATES; RABBITS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 77952026993     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2009.0226     Document Type: Article

References (14)

1. Vellonen KS, Honkakoski P, Urtti A: Substrates and inhibitors of eff ux proteins interfere with the MTT assay in cells and may lead to underestimation of drug toxicity. Eur J Pharm Sci 2004; 23:181-188.
2. Pozzolini M, Scarf S, Benatti U, Giovine M: Interference in MTT cell viability assay in activated macrophage cell line. Anal Biochem 2003; 313:338-341.
3. Bruggisser R, von Daeniken K, Jundt G, Schaffner W, Tullberg-Reinert H: Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med 2002; 68:445-448.
4. Wang X, Ge J, Wang K, Qian J, Zou Y: Evaluation of MTT assay for measurement of emodin-induced cytotoxicity. Assay Drug Dev Technol 2006; 4:203-207.
5. Ulukaya E, Ozdikicioglu F, Oral AY, Demirci M: The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested. Toxicol In Vitro 2008; 22:232-239.
6. Hamid R, Rotshteyn Y, Rabadi L, Parikh R, Bullock P: Comparison of alamar blue and MTT assays for high through-put screening. Toxicol In Vitro 2004; 18:703-710.
7. Riss TL, Moravec RA: use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev Technol 2004; 2:51-62.
8. Geschwind JF, Ko YH, Torbenson MS, Magee C, Pedersen PL: Novel therapy for liver cancer: direct intraarterial injection of a potent inhibitor of ATP production. Cancer Res 2002; 62:3909-3913.
9. Vali M, Vossen JA, Buijs M, Engles JM, Liapi E, Ventura V P, et al.: Targeting of VX2 rabbit liver tumor by selective delivery of 3-bromopyruvate: a biodistribution and survival study. J Pharmacol Exp Ther 2008; 327:32-37.
10. Dell'antone P: Targets of 3-bromopyruvate, a new, energy depleting, anticancer agent. Med Chem 2009.
11. Cao X, Bloomston M, Zhang T, Frankel WL, Jia G, Wang B, et al.: Synergistic antipancreatic tumor effect by simultaneously targeting hypoxic cancer cells with HSP90 inhibitor and glycolysis inhibitor. Clin Cancer Res 2008; 14:1831-1839.
12. Marshall NJ, Goodwin CJ, Holt SJ: A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth Regul 1995; 5:69-84.
13. Hatok J, Babusikova E, Matakova T, Mistuna D, Dobrota D, Racay P: In vitro assays for the evaluation of drug resistance in tumor cells. Clin Exp Med 2009; 9:1-7.
14. Berridge MV, Herst PM, Tan AS: Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 2005; 11:127-152.