Beclin-1 expression in normal bladder and in Cd2+ and As3+ exposed and transformed human urothelial cells (UROtsa)

Toxicology Letters

Volumn 195, Issue 1, 2010, Pages 15-22

  Larson, Jennifer L ^a   Somji, Seema ^a   Zhou, Xu Dong ^a   Sens, Mary Ann ^a   Garrett, Scott H ^a   Sens, Donald A ^a   Dunlevy, Jane R ^a  

^a UNIVERSITY OF NORTH DAKOTA SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

Arsenite; Autophagy; Beclin 1; Bladder; Cadmium

Indexed keywords

ARSENIC TRIOXIDE; AUTOPHAGY PROTEIN 12; AUTOPHAGY PROTEIN 5; AUTOPHAGY PROTEIN 7; BECLIN 1; CADMIUM; MEMBRANE PROTEIN; MESSENGER RNA; PROTEIN LC3B; UNCLASSIFIED DRUG; APOPTOSIS REGULATORY PROTEIN; ARSENIC; BECN1 PROTEIN, HUMAN;

ARTICLE; AUTOPHAGY; CELL TRANSFORMATION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MALIGNANT TRANSFORMATION; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; REAL TIME POLYMERASE CHAIN REACTION; UROTHELIUM; WESTERN BLOTTING; BLADDER; CELL LINE; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; TIME;

APOPTOSIS REGULATORY PROTEINS; ARSENIC; CADMIUM; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; HUMANS; MEMBRANE PROTEINS; RNA, MESSENGER; TIME FACTORS; URINARY BLADDER; UROTHELIUM;

EID: 77951921014     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2010.02.021     Document Type: Article

References (20)

1. Aita V.M., Liang X.H., Murty V.V., Pincus D.L., Yu W., Cayanis E., Kalachikov S., Gilliam T.C., Levine B. Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics 1999, 59:59-65.
2. Cao, L., Zhou, X.D., Sens, M.A., Garrett, S.H., Zheng, Y., Dunlevy, J.R., Sens, D.A., Somji, S., in press. Keratin 6 expression correlates to areas of squamous differentiation in multiple independent isolates of As3+-induced bladder cancer. J. Appl. Toxicol.
3. Chai C.Y., Huang Y.C., Hung W.C., Kang W.Y., Chen W.T. Arsenic salts induce autophagic cell death and hypermethylation of DAPK promoter in SV-40 immortalized human uroepithelial cells. Toxicol. Lett. 2007, 173:48-56.
4. 2+ induces metallothionein protein accumulation but not metallothionein isoform 2 mRNA. Environ. Health Perspect. 1998, 106:587-595.
5. Kroemer G., Levine B. Autophagic cell death: the story of a misnomer. Nat. Rev. Mol. Cell Biol. 2008, 9:1004-1010.
6. Maluri M.C., Tasemir E., Criollo A., Morselli E., Vicencio J.M., Carnuccio R., Kroemer G. Control of autophagy by oncogenes and tumor suppressor genes. Cell Death Differ. 2009, 16:87-93.
7. Petzoldt J.L., Leigh I.M., Duffy P.G., Masters J.R.W. Culture and characterization of human urothelium in vivo and in vitro. Urol. Res. 1994, 22:67-74.
8. Petzoldt J.L., Leigh I.M., Duffy P.C., Sexton C., Masters J.R.W. Immortalization of human urothelial cells. Urol. Res. 1995, 23:377-380.
9. Pickford F., Masliah E., Britschgi M., Lucin K., Narasimhan R., Jaeger P., Small S., Spencer B., Rockenstein E., Levine B., Wyss-Coray T. The autophagy-related protein Beclin 1 shows reduced expression in early Alzheimer disease and regulates amyloid beta accumulation in mice. J. Clin. Invest. 2008, 118:2190-2199.
10. Qu X., Yu J., Bhagat G., Furuya N., Hibshoosh H., Troxel A., Rosen J., Eskelinen E.L., Mizushima N., Ohsumi Y., Cattoretti G., Levine B. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J. Clin. Invest. 2003, 112:1809-1820.
11. Rossi M.R., Masters J.R.W., Park S., Todd J.H., Garrett S.H., Sens M.A., Somji S., Nath J., Sens D.A. The immortalized UROtsa cell line as a potential cell culture model of human urothelium. Environ. Health Perspect. 2001, 109:801-808.
12. Satarug S., Garrett S.H., Sens M.A., Sens D.A. Cadmium, environmental exposure and health outcomes. Environ. Health Perspect. 2010, 118:182-190.
13. Sens D.A., Park S., Gurel V., Sens M.A., Garrett S.H., Somji S. Inorganic cadmium- and arsenite-induced malignant transformation of human bladder urothelial cells. Toxicol. Sci. 2004, 79:56-63.
14. Sinha S., Levine B. The autophagy effector Beclin-1: a novel BH-3 only protein. Oncogene 2008, 27:S137-148.
15. 2+-exposed human proximal tubule cells. Toxicol. Sci. 2004, 80:358-366.
16. 3+-induced cell death. Toxicol. Sci. 2006, 94:293-301.
17. Somji S., Zhou X.D., Mehus A., Sens M.A., Garrett S.H., Lutz K.L., Dunlevy J.R., Zheng Y., Sens D.A. Variation of keratin 7 expression and other phenotypic characteristics of independent isolates of cadmium transformed human urothelial cells (UROtsa). Chem. Res. Toxicol. 2010, 23:348-356.
18. Tannous P., Zhu H., Johnstone J.L., Shelton J.M., Rajasekaran N.S., Benjamin I.J., Nguyen L., Gerard R.D., Levine B., Rothermel B.A., Hill J.A. Autophagy is an adaptive response in desmin-related cardiomyopathy. Proc. Natl. Acad. Sci. U.S.A. 2008, 105:9745-9750.
19. Todde V., Veenhuis M., van der Klei I.J. Autophagy: principles and significance in health and disease. Biochim. Biophys. Acta 2009, 1792:2-13.
20. Yue Z., Jin S., Yang C., Levine A.J., Heintz N. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:15077-15082.