Role of cysteine at positions 67, 161 and 241 of a Bacillus sphaericus binary toxin BinB

BMB Reports

Volumn 43, Issue 1, 2010, Pages 23-28

  Boonyos, Patcharaporn ^a   Soonsanga, Sumarin ^b   Boonserm, Panadda ^a   Promdonkoy, Boonhiang ^b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

Author keywords

Bacillus sphaericus; Binary toxin; Disulfide bond; Mosquito larvicidal toxin; Mutagenesis

Indexed keywords

BACILLUS SPHAERICUS;

BACTERIAL TOXIN; BINB PROTEIN, BACILLUS SPHAERICUS; CYSTEINE;

AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; BACILLUS; CHEMISTRY; DRUG EFFECT; GENETICS; LARVA; METABOLISM; MOSQUITO; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ANIMALS; BACILLUS; BACTERIAL TOXINS; CULICIDAE; CYSTEINE; LARVA; MOSQUITO CONTROL; MUTAGENESIS, SITE-DIRECTED;

EID: 77951888117     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2010.43.1.023     Document Type: Article

References (30)

1. Charles, J. F., Nielson-LeRoux, C. and Delecluse, A. (1996) Bacillus sphaericus toxins: molecular biology and mode of action. Annu. Rev. Entomol. 41, 451-472.
2. Nicolas, L., Nielsen-Leroux, C., Charles, J. F. and Delecluse, A. (1993) Respective role of the 42-and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis. FEMS Microbiol. Lett. 106, 275-280.
3. Baumann, P., Clark, M. A., Baumann, L. and Broadwell, A. H. (1991) Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins. Microbiol. Rev. 55, 425-436.
4. Baumann, L., Broadwell, A. H. and Baumann, P. (1988) Sequence analysis of the mosquitocidal toxin genes encoding 51.4-and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297. J. Bacteriol. 170, 2045-2050.
5. Hire, R. S., Hadapad, A. B., Dongre, T. K. and Kumar, V. (2009) Purification and characterization of mosquitocidal Bacillus sphaericus BinA protein. J. Invertebr. Pathol. 101, 106-111.
6. Broadwell, A. H. and Baumann, P. (1987) Proteolysis in the gut of mosquito larvae results in further activation of the Bacillus sphaericus toxin. Appl. Environ. Microbiol. 53, 1333-1337.
7. Elangovan, G., Shanmugavelu, M., Rajamohan, F., Dean, D. H. and Jayaraman, K. (2000) Identification of the functional site in the mosquito larvicidal binary toxin of Bacillus sphaericus 1593M by site-directed mutagenesis. Biochem. Biophys. Res. Commun. 276, 1048-1055.
8. Oei, C., Hindley, J. and Berry, C. (1992) Binding of purified Bacillus sphaericus binary toxin and its deletion derivatives to Culex quinquefasciatus gut: elucidation of functional binding domains. J. Gen. Microbiol. 138, 1515-1526.
9. Darboux, I., Nielsen-LeRoux, C., Charles, J. F. and Pauron, D. (2001) The receptor of Bacillus sphaericus binary toxin in Culex pipiens (Diptera: Culicidae) midgut: molecular cloning and expression. Insect Biochem. Mol. Biol. 31, 981-990.
10. Silva-Filha, M. H., Nielsen-LeRoux, C. and Charles, J. F. (1999) Identification of the receptor for Bacillus sphaericus crystal toxin in the brush border membrane of the mosquito Culex pipiens (Diptera: Culicidae). Insect Biochem. Mol. Biol. 29, 711-721.
11. Chiou, C., Davidson, E. W., Thanabalu, T., Porter, A. G. and Allen, J. P. (1999) Crystallization and preliminary X-ray diffraction studies of the 51 kDa protein of the mosquito-larvicidal binary toxin from Bacillus sphaericus. Acta Crystallogr. D. Biol. Crystallogr. 55, 1083-1085.
12. Smith, A. W., Camara-Artigas, A. and Allen, J. P. (2004) Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus. Acta Crystallogr. D. Biol. Crystallogr. 60, 952-953.
13. Smith, A. W., Camara-Artigas, A., Brune, D. C. and Allen, J. P. (2005) Implications of high-molecular-weight oligomers of the binary toxin from Bacillus sphaericus. J. Invertebr. Pathol. 88, 27-33.
14. Berry, C., Hindley, J., Ehrhardt, A. F., Grounds, T., de Souza, I. and Davidson, E. W. (1993) Genetic determinants of host ranges of Bacillus sphaericus mosquito larvicidal toxins. J. Bacteriol. 175, 510-518.
15. Yuan, Z., Rang, C., Maroun, R. C., Juarez-Perez, V., Frutos, R., Pasteur, N., Vendrely, C., Charles, J. F. and Nielsen-Leroux, C. (2001) Identification and molecular structural prediction analysis of a toxicity determinant in the Bacillus sphaericus crystal larvicidal toxin. Eur. J. Biochem. 268, 2751-2760.
16. Sanitt, P., Promdonkoy, B. and Boonserm, P. (2008) Targeted mutagenesis at charged residues in Bacillus sphaericus BinA toxin affects mosquito-larvicidal activity. Curr. Microbiol. 57, 230-234.
17. Promdonkoy, B., Promdonkoy, P., Wongtawan, B., Boonserm, P. and Panyim, S. (2008) Cys31, Cys47, and Cys195 in BinA are essential for toxicity of a binary toxin from Bacillus sphaericus. Curr. Microbiol. 56, 334-338.
18. Clark, M. A. and Baumann, P. (1990) Deletion analysis of the 51-kilodalton protein of the Bacillus sphaericus 2362 binary mosquitocidal toxin: construction of derivatives equivalent to the larva-processed toxin. J. Bacteriol. 172, 6759-6763.
19. Hogg, P. J. (2003) Disulfide bonds as switches for protein function. Trends Biochem. Sci. 28, 210-214.
20. Kadokura, H., Katzen, F. and Beckwith, J. (2003) Protein disulfide bond formation in prokaryotes. Annu. Rev. Biochem. 72, 111-135.
21. Boonserm, P., Mo, M., Angsuthanasombat, C. and Lescar, J. (2006) Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution. J. Bacteriol. 188, 3391-3401.
22. Leung, M. Y. and Ho, W. K. (2006) Substitution of serine for non-disulphide-bond-forming cysteine in grass carp (Ctenopharygodon idellus) growth hormone improves in vitro oxidative renaturation. J. Biochem. Mol. Biol. 39, 150-157.
23. Shanmugavelu, M., Rajamohan, F., Kathirvel, M., Elangovan, G., Dean, D. H. and Jayaraman, K. (1998) Functional complementation of nontoxic mutant binary toxins of Bacillus sphaericus 1593M generated by site-directed mutagenesis. Appl. Environ. Microbiol. 64, 756-759.
24. Boonserm, P., Moonsom, S., Boonchoy, C., Promdonkoy, B., Parthasarathy, K. and Torres, J. (2006) Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers. Biochem. Biophys. Res. Commun. 342, 1273-1278.
25. Schwartz, J. L., Potvin, L., Coux, F., Charles, J. F., Berry, C., Humphreys, M. J., Jones, A. F., Bernhart, I., Dalla, S. M. and Menestrina, G. (2001) Permeabilization of model lipid membranes by Bacillus sphaericus mosquitocidal binary toxin and its individual components. J. Membr. Biol. 184, 171-183.
26. Cokmus, C., Davidson, E. W. and Cooper, K. (1997) Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells. J. Invertebr. Pathol. 69, 197-204.
27. Promdonkoy, B., Promdonkoy, P. and Panyim, S. (2008) High-level expression in Escherichia coli, purification and mosquito-larvicidal activity of the binary toxin from Bacillus sphaericus. Curr. Microbiol. 57, 626-630.
28. Promdonkoy, B., Promdonkoy, P., Audtho, M., Tanapongpipat, S., Chewawiwat, N., Luxananil, P. and Panyim, S. (2003) Efficient expression of the mosquito larvicidal binary toxin gene from Bacillus sphaericus in Escherichia coli. Curr. Microbiol. 47, 383-387.
29. Thammachat, S., Pathaichindachote, W., Krittanai, C. and Promdonkoy, B. (2008) Amino acids at N-and C-termini are required for the efficient production and folding of a cytolytic delta-endotoxin from Bacillus thuringiensis. BMB. Rep. 41, 820-825.
30. Finney, D. (1971) Probit Analysis, Cambridge University Press, London, UK.