Efficient expression of the mosquito larvicidal binary toxin gene from Bacillus sphaericus in Escherichia coli

Current Microbiology

Volumn 47, Issue 5, 2003, Pages 383-387

  Promdonkoy, Boonhiang ^a   Promdonkoy, Patcharee ^a   Audtho, Mongkon ^a   Tanapongpipat, Sutipa ^a   Chewawiwat, Namchai ^a   Luxananil, Plearnpis ^a   Panyim, Sakol ^b  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b MAHIDOL UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; BINA TOXIN; BINB TOXIN; LARVICIDAL AGENT; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SPHAERICUS; BACTERIAL STRAIN; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN STABILITY;

BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 0141669021     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00284-003-4035-3     Document Type: Article

References (20)

1. Ahmad S, Selvapandiyan A, Bhatnagar RK (1998) Increased toxicity of modified mosquitocidal binary toxins of Bacillus sphaericus expressed in Escherichia coli. Appl Microbiol Biotechnol 49:164-167
2. Baumann L, Broadwell AH, Baumann P (1988) Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297. J Bacteriol 170:2045-2050
3. Baumann P, Clark MA, Baumann L, Broadwell AH (1991) Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins. Microbiol Rev 55:425-436
4. Broadwell AH, Baumann L, Baumann P (1990) The 42- and 51-kilodalton mosquitocidal proteins of Bacillus sphaericus 2362: construction of recombinants with enhanced expression and in vivo studies of processing and toxicity. J Bacteriol 172:2217-2223
5. Charles J-F, Nielsen-LeRoux C, Delecluse A (1996) Bacillus sphaericus toxins: molecular biology and mode of action. Annu Rev Entomol 41:451-472
6. Charles J-F, Silva-Filha MH, Nielsen-LeRoux C, Humphreys MJ, Berry C (1997) Binding of the 51- and 42-kDa individual components from the Bacillus sphaericus crystal toxin to mosquito larval midgut membranes from Culex and Anopheles sp. (Diptera: Culicidae). FEMS Microbiol Lett 156:153-159
7. Dower WJ, Miller JF, Ragsdale CW (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16:6127-6145
8. Finney D (1971) Probit analysis, 3rd edn. London: Cambridge University Press
9. Khampang P, Chungjatupornchai W, Luxananil P, Panyim S (1999) Efficient expression of mosquito-larvicidal proteins in a gram-negative bacterium capable of recolonization in the guts of Anopheles dirus larva. Appl Microbiol Biotechnol 51:79-84
10. Khampang P, Luxananil P, Tanapongpipat S, Chungjatupornchai W, Panyim S (2001) Recombinant Enterobacter amnigenus highly toxic to Anopheles dirus mosquito larvae. Curr Microbiol 43:448-451
11. Liu J-W, Porter AG, Wee BY, Thanabalu T (1996) New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins. Appl Environ Microbiol 62:2174-2176
12. Porter AG, Davidson EW. Liu J-W (1993) Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes. Microbiol Rev 57:838-861
13. Sangthongpitag K, Penfold RJ, Delaney SF, Rogers PL (1997) Cloning and expression of the Bacillus sphaericus 2362 mosquitocidal genes in a non-toxic unicellular cyanobacterium, Synechococcus pCC6301. Appl Microbiol Biotechnol 47:379-384
14. Schnepf HE, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal proteins. Microbiol Mol Biol Rev 62:775-806
15. Shi Y, Yuan Z, Cai Q, Yu J, Yan J, Pang Y (2001) Cloning and expression of the binary toxin gene from Bacillus sphaericus IAB872 in a crystal-minus Bacillus thuringiensis subsp. israelensis. Curr Microbiol 43:21-25
16. Studier FW (1991) Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J Mol Biol 219:37-44
17. Thanabalu T, Hindley J, Jackson-Yap J, Berry C (1991) Cloning, sequencing and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1. J Bacteriol 173:2776-2785
18. Towbin H, Staehelin T, Gordon J (1979) Electrophoresis transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
19. Xu X, Yan G, Kong R, Liu X, Yu L (2000) Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control. Curr Microbiol 41:352-356
20. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13MP18 and pUC19 vectors. Gene 33:103-119