Fluorescent protein recruitment assay for demonstration and analysis of in vivo protein interactions in plant cells and its application to Tobacco mosaic virus movement protein

Plant Journal

Volumn 62, Issue 1, 2010, Pages 171-177

  Boutant, Emmanuel ^a   Didier, Pascal ^a   Niehl, Annette ^a   Mély, Yves ^a   Ritzenthaler, Christophe ^a   Heinlein, Manfred ^a,b  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

GFP; In vivo protein interactions; Movement protein; Tobacco mosaic virus

Indexed keywords

FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENT PROTEIN; IN-PLANTS; IN-VIVO; LIVING CELL; MOVEMENT PROTEINS; PROTEIN INTERACTION; RE-LOCALIZATION; TOBACCO MOSAIC VIRUS;

FLUORESCENCE; TOBACCO; VIRUSES;

PROTEINS;

PHOTOPROTEIN; PLANT VIRUS MOVEMENT PROTEIN;

ARTICLE; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; PROTEIN ANALYSIS; TOBACCO; TOBACCO MOSAIC VIRUS; TRANSGENIC PLANT; VIROLOGY;

LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PLANT VIRAL MOVEMENT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROTEIN INTERACTION MAPPING; TOBACCO; TOBACCO MOSAIC VIRUS;

TOBACCO MOSAIC VIRUS;

EID: 77951799558     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2010.04126.x     Document Type: Article

References (21)

1. Ashby, J., Boutant, E., Seemanpillai, M., Groner, A., Sambade, A., Ritzenthaler, C. Heinlein, M. (2006) Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein. J. Virol. 80, 8329 8344. (Pubitemid 44384846)
2. Bacia, K. Schwille, P. (2007) Practical guidelines for dual-color fluorescence cross-correlation spectroscopy. Nat. Protoc. 2, 2842 2856.
3. Boyko, V., Ferralli, J., Ashby, J., Schellenbaum, P. Heinlein, M. (2000a) Function of microtubules in intercellular transport of plant virus RNA. Nat. Cell Biol. 2, 826 832.
4. Boyko, V., van der Laak, J., Ferralli, J., Suslova, E., Kwon, M.-O. Heinlein, M. (2000b) Cellular targets of functional and dysfunctional mutants of Tobacco mosaic virus movement protein fused to GFP. J. Virol. 74, 11339 11346.
5. Boyko, V., Ashby, J.A., Suslova, E., Ferralli, J., Sterthaus, O., Deom, C.M. Heinlein, M. (2002) Intramolecular complementing mutations in Tobacco mosaic virus movement protein confirm a role for microtubule association in viral RNA transport. J. Virol. 76, 3974 3980.
6. Brandner, K., Sambade, A., Boutant, E., Didier, P., Mély, Y., Ritzenthaler, C. Heinlein, M. (2008) TMV movement protein interacts with GFP-tagged microtubule end-binding protein 1 (EB1). Plant Physiol. 147, 611 623.
7. Brill, L.M., Nunn, R.S., Kahn, T.W., Yeager, M. Beachy, R.N. (2000) Recombinant Tobacco mosaic virus movement protein is an RNA-binding, α-helical membrane protein. Proc. Natl. Acad. Sci. USA, 97, 7112 7117.
8. Brill, L.M., Dechongkit, S., DeLaBarre, B., Stroebel, J., Beachy, R.N. Yeager, M. (2004) Dimerization of recombinant Tobacco mosaic virus movement protein. J. Virol. 78, 3372 3377.
9. Christensen, N., Tilsner, J., Bell, K., Hammann, P., Parton, R., Lacomme, C. Oparka, K. (2009) The 5′cap of Tobacco mosaic virus (TMV) is required for virion attachment to the actin/ER network during early infection. Traffic, 10, 536 551.
10. Corbin, B.D., Geissler, B., Sadasivam, M. Margolin, W. (2004) Z-ring-independent interaction between a subdomain of FtsA and late septation proteins as revealed by a polar recruitment assay. J. Bacteriol. 186, 7736 7744.
11. Deom, C.M., Oliver, M.J. Beachy, R.N. (1987) The 30-kilodalton gene product of Tobacco mosaic virus potentiates virus movement. Science, 237, 384 389.
12. Ding, Z., Zhao, Z., Jakubowski, S.J., Krishnamohan, A., Margolin, W. Christie, P.J. (2002) A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a type IV secretion system. J. Bacteriol. 184, 5572 5582.
13. Fricker, M., Runions, J. Moore, I. (2006) Quantitative fluorescence microscopy: from art to science. Annu. Rev. Plant Biol. 57, 79 107.
14. Heinlein, M., Padgett, H.S., Gens, J.S., Pickard, B.G., Casper, S.J., Epel, B.L. Beachy, R.N. (1998) Changing patterns of localization of the Tobacco mosaic virus movement protein and replicase to the endoplasmic reticulum and microtubules during infection. Plant Cell, 10, 1107 1120.
15. Hofmann, C., Niehl, A., Sambade, A., Steinmetz, A. Heinlein, M. (2009) Inhibition of TMV movement by expression of an actin-binding protein. Plant Physiol. 149, 1810 1823.
16. Karimi, M., Inzé, D. Depicker, A. (2002) Gateway vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 7, 193 195.
17. Palmer, E. Freeman, T. (2004) Investigation into the use of C- and N-terminal GFP fusion proteins for subcellular localization studies using reverse transfection microarrays. Comp. Funct. Genomics, 5, 342 353.
18. Roy, A.A., Lemberg, K.E. Chidiac, P. (2003) Recruitment of RGS2 and RGS4 to the plasma membrane by G proteins and receptors reflects functional interactions. Mol. Pharmacol. 64, 587 593.
19. Sambade, A., Brandner, K., Hofmann, C., Seemanpillai, M., Mutterer, J. Heinlein, M. (2008) Transport of TMV movement protein particles associated with the targeting of RNA to plasmodesmata. Traffic, 9, 2073 2088.
20. Schmitz, A.M., Morrison, M.F., Agunwamba, A.O., Nibert, M.L. Lesser, C.F. (2009) Protein interaction platforms: visualization of interacting proteins in yeast. Nat. Methods, 6, 500 502.
21. Vogler, H., Kwon, M.O., Dang, V., Sambade, A., Fasler, M., Ashby, J. Heinlein, M. (2008) Tobacco mosaic virus movement protein enhances the spread of RNA silencing. PLoS Pathog. 4, e1000038.