Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair

International Journal of Molecular Medicine

Volumn 25, Issue 5, 2010, Pages 701-708

  Kuhne, Maren ^a   John, Thilo ^a   El Sayed, Karym ^a,b   Marzahn, Ulrike ^b   Aue, Annekatrin ^a,b   Kohl, Benjamin ^a   Stoelzel, Katharina ^b   Ertel, Wolfgang ^a   Blottner, Dieter ^a   Haisch, Andreas ^b   Schulze Tanzil, Gundula ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Head and Neck Surgery at UKB   (Germany)

Author keywords

Alginate; Chondrocyte; Co culture; Sox9; Type II collagen

Indexed keywords

BETA1 INTEGRIN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; ELASTIN; LUBRICIN; PROTEIN; TRANSCRIPTION FACTOR SOX9; UNCLASSIFIED DRUG;

ADULT; AGED; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; AUTOTRANSPLANTATION; CARTILAGE CELL; CARTILAGE INJURY; CARTILAGE MATRIX; CELL PROLIFERATION; CLINICAL ARTICLE; COCULTURE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FLOW CYTOMETRY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOFLUORESCENCE TEST; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ALGINATES; ANIMALS; CARTILAGE, ARTICULAR; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CHONDROCYTES; COCULTURE TECHNIQUES; COLLAGEN TYPE II; EAR CARTILAGE; HUMANS; REGENERATION; SWINE;

EID: 77951760335     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm_00000394     Document Type: Article

References (32)

1. Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O and Peterson L: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331: 889-895, 1994. (Pubitemid 24299869)
2. Brittberg M: Autologous chondrocyte transplantation. Clin Orthop Relat Res (Suppl) 391: S147-S155, 1999.
3. Benya PD and Shaffer JD: Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215-224, 1982.
4. Malpeli M, Randazzo N, Cancedda R and Dozin B: Serum-free growth medium sustains commitment of human articular chondrocyte through maintenance of Sox9 expression. Tissue Eng 10: 145-155, 2004. (Pubitemid 38199370)
5. Tew SR and Hardingham TE: Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization. J Biol Chem 281: 39471-39479, 2006.
6. Lefebvre V, Huang W, Harley VR, Goodfellow PN and de Crombrugghe B: SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene. Mol Cell Biol 17: 2336-2346, 1997. (Pubitemid 27133320)
7. Sekiya I, Tsuji K, Koopman P, et al: SOX9 enhances aggrecan gene promoter/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6. J Biol Chem 275: 10738-10744, 2000. (Pubitemid 30212701)
8. Kolettas E, Muir HI, Barrett JC and Hardingham TE: Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor. Rheumatology 40: 1146-1156, 2001. (Pubitemid 32998399)
9. Haisch A, Marzahn U, Mobasheri A, Schulze-Tanzil G and Shakibaei M: Development and phenotypic characterization of a high density in vitro model of auricular chondrocytes with applications in reconstructive plastic surgery. Histol Histopathol 21: 467-476, 2006.
10. Schulze-Tanzil G, de Souza P, Villegas Castrejon H, et al: Redifferentiation of dedifferentiated human chondrocytes in high-density cultures. Cell Tissue Res 308: 371-379, 2002. (Pubitemid 34666963)
11. Schulze-Tanzil G, Mobasheri A, de Souza P, John T and Shakibaei M: Loss of chondrogenic potential in dedifferentiated chondrocytes correlates with deficient Shc-Erk interaction and apoptosis. Osteoarthritis Cartilage 12: 448-458, 2004. (Pubitemid 38696945)
12. Schulze-Tanzil G, Muller RD, Kohl B, et al: Differing in vitro biology of equine, ovine, porcine and human articular chondrocytes derived from the knee joint: an immunomorphological study. Histochem Cell Biol 131: 219-229, 2009.
13. Gelse K, Brem M, Klinger P, et al: Paracrine effect of transplanted rib chondrocyte spheroids supports formation of secondary cartilage repair tissue. J Orthop Res 27: 1216-1225, 2009.
14. Malicev E, Marolt D, Kregar Velikonja N, Kreft ME, Drobnic M and Rode M: Growth and differentiation of alveolar bone cells in tissue-engineered constructs and monolayer cultures. Biotechnol Bioeng 100: 773-781, 2008. (Pubitemid 351914003)
15. Candrian C, Vonwil D, Barbero A, et al: Engineered cartilage generated by nasal chondrocytes is responsive to physical forces resembling joint loading. Arthritis Rheum 58: 197-208, 2008.
16. Johnson TS, Xu JW, Zaporojan VV, et al: Integrative repair of cartilage with articular and nonarticular chondrocytes. Tissue Eng 10: 1308-1315, 2004. (Pubitemid 39557835)
17. Szeparowicz P, Popko J, Sawicki B and Wolczynski S: Is the repair of articular cartilage lesion by costal chondrocyte transplantation donor age-dependent? An experimental study in rabbits. Folia Histochem Cytobiol 44: 201-206, 2006. (Pubitemid 44131989)
18. van Osch GJ, Mandl EW, Jahr H, Koevoet W, Nolst-Trenite G and Verhaar JA: Considerations on the use of ear chondrocytes as donor chondrocytes for cartilage tissue engineering. Biorheology 41: 411-421, 2004. (Pubitemid 39277098)
19. Vinatier C, Gauthier O, Fatimi A, et al: An injectable cellulose-based hydrogel for the transfer of autologous nasal chondrocytes in articular cartilage defects. Biotechnol Bioeng 102: 1259-1267, 2009.
20. Vinatier C, Gauthier O, Masson M, et al: Nasal chondrocytes and fibrin sealant for cartilage tissue engineering. J Biomed Mater Res 89: 176-185, 2009.
21. Grad S and Salzmann GM: Chondrocytes - one cell type, different subpopulations: Characteristics and behavior of different types of chondrocytes and implications for tissue engineering applications. Orthopade (In press).
22. Gigante A, Specchia N and Greco F: Age-related distribution of elastic fibers in the rabbit knee. Clin Orthop Relat Res 308: 33-42, 1994. (Pubitemid 24349306)
23. Flannery CR, Hughes CE, Schumacher BL, et al: Articular cartilage superficial zone protein (SZP) is homologous to mega-karyocyte stimulating factor precursor and is a multifunctional proteoglycan with potential growth-promoting, cytoprotective, and lubricating properties in cartilage metabolism. Biochem Biophys Res Commun 254: 535-541, 1999.
24. Rhee DK, Marcelino J, Baker M, et al: The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth. J Clin Invest 115: 622-631, 2005. (Pubitemid 40322236)
25. Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: E45, 2001.
26. Henderson JH, Welter JF, Mansour JM, Niyibizi C, Caplan AI and Dennis JE: Cartilage tissue engineering for laryngotracheal reconstruction: comparison of chondrocytes from three anatomic locations in the rabbit. Tissue Eng 13: 843-853, 2007. (Pubitemid 46651514)
27. Tay AG, Farhadi J, Suetterlin R, Pierer G, Heberer M and Martin I: Cell yield, proliferation, and postexpansion differentiation capacity of human ear, nasal, and rib chondrocytes. Tissue Eng 10: 762-770, 2004. (Pubitemid 38880394)
28. Isogai N, Kusuhara H, Ikada Y, et al: Comparison of different chondrocytes for use in tissue engineering of cartilage model structures. Tissue Eng 12: 691-703, 2006. (Pubitemid 43726514)
29. Xu JW, Zaporojan V, Peretti GM, et al: Injectable tissue-engineered cartilage with different chondrocyte sources. Plast Reconstr Surg 113: 1361-1371, 2004. (Pubitemid 39201866)
30. Malicev E, Kregar-Velikonja N, Barlic A, Alibegovic A and Drobnic M: Comparison of articular and auricular cartilage as a cell source for the autologous chondrocyte implantation. J Orthop Res 27: 943-948, 2009.
31. Chung C, Erickson IE, Mauck RL and Burdick JA: Differential behavior of auricular and articular chondrocytes in hyaluronic acid hydrogels. Tissue Eng Part A 14: 1121-1131, 2008. (Pubitemid 351931507)
32. Zhang L and Spector M: Comparison of three types of chondrocytes in collagen scaffolds for cartilage tissue engineering. Biomed Mater 4: 45012, 2009.