SeqSharp: A general approach for improving cycle-sequencing that facilitates a robust one-step combined amplification and sequencing method

Journal of Molecular Diagnostics

Volumn 12, Issue 3, 2010, Pages 272-277

  SenGupta, Dhruba J ^a   Cookson, Brad T ^a  

^a UNIVERSITY OF WASHINGTON MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; HUMAN; LIGASE CHAIN REACTION; NONHUMAN; REGULATOR GENE; SEQSHARP METHOD; SEQUENCE DATABASE;

BACTERIA (MICROORGANISMS);

EID: 77951741562     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2010.090134     Document Type: Article

References (19)

1. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977, 74:5463-5467
2. ten Bosch JR, Grody WW: Keeping up with the next generation: massively parallel sequencing in clinical diagnostics. J Mol Diagn 2008, 10:484-492
3. Innis MS, Myambo KB, Gelfand DH, Brown MA: DNA sequencing with Thermus acquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. 1988. Biotechnology 1992, 24:6-10
4. Rapley R (Ed): PCR Sequencing Protocols: Methods in Molecular Biology, vol 65. New York, Humana Press, 1996
5. Dugan KA, Lawrence HS, Hares DR, Fisher CL, Budowle B: An improved method for post-PCR purification for mtDNA sequence analysis. J Forensic Sci 2002, 47:811-818
6. Smith PJ, Ballantyne J: Simplified low-copy-number DNA analysis by post-PCR purification. J Forensic Sci 2007, 52:820-829
7. Ruano G, Kidd KK: Coupled amplification and sequencing of genomic DNA. Proc Natl Acad Sci USA 1991, 88:2815-2819 (Pubitemid 21916510)
8. Kilger C, Paabo S: Direct exponential amplification and sequencing (DEXAS) of genomic DNA. Biol Chem 1997, 378:99-105 (Pubitemid 27166043)
9. Murphy KM, Eshleman JR: Simultaneous sequencing of multiple polymerase chain reaction products and combined polymerase chain reaction with cycle sequencing in single reactions. Am J Pathol 2002, 161:27-33
10. Shopsin B, Gomez M, Montgomery SO, Smith DH, Waddington M, Dodge DE, Bost DA, Riehman M, Naidich S, Kreiswirth BN: Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol 1999, 37:3556-3563 (Pubitemid 29491091)
11. Murphy KM, Berg KD, Eshleman JR: Sequencing of genomic DNA by combined amplification and cycle sequencing reaction. Clin Chem 2005, 51:35-39
12. Kurtzman CP, Robnett CJ: Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998, 73:331-371
13. Stackebrandt E, Goebel BM: Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriolog. Int J Syst Bacteriology 1994, 44:846-849 (Pubitemid 24315169)
14. Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, Raoult D: 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 2000, 38:3623-3630
15. Clarridge JE 3rd: Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 2004, 17:840-862
16. Mitsis PG, Kwagh JG: Characterization of the interaction of lambda exonuclease with the ends of DNA. Nucleic Acids Res 1999, 27: 3057-3063
17. Boissinot K, Huletsky A, Peytavi R, Turcotte S, Veillette V, Boissinot M, Picard FJ, Martel EA, Bergeron MG: Rapid exonuclease digestion of PCR-amplified targets for improved microarray hybridization. Clin Chem 2007, 53:2020-2023 (Pubitemid 350036061)
18. Motz M, Paabo S, Kilger C: Improved cycle sequencing of GC-rich templates by a combination of nucleotide analogs. Biotechniques 2000, 29:268-270 (Pubitemid 30658278)
19. Werle E, Schneider C, Renner M, Volker M, Fiehn W: Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Res 1994, 22:4354-4355 (Pubitemid 24322750)