Direct exponential amplification and sequencing (DEXAS) of genomic DNA

Biological Chemistry

Volumn 378, Issue 2, 1997, Pages 99-105

  Kilger, Christian ^a   Pääbo, Svante ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Cycle sequencing; DEXAS

Indexed keywords

CELL DNA; DEOXYRIBONUCLEOTIDE;

ARTICLE; AUTOANALYSIS; CONTROLLED STUDY; DNA PURIFICATION; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; HUMAN; METHODOLOGY; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

EID: 0342981450     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/bchm.1997.378.2.99     Document Type: Article

References (12)

1. Anderson, S., Bankier, A.T., Barrell, B.G., de Bruijn, M.H.L., Coulson, A.R., Drouin, J., Eperon, I.C., Nierlich, D.P., Roe, B.A., Sanger, F., Schreier, P.H., Smith, A.J.H., Staden, R., and Young, I.G. (1981). Sequence and organization of the human mitochondrial genome. Nature 290, 457-465.
2. Birnboim, H.C., and Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513-1523.
3. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L., and Boyer, H.W. (1977). Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95-113.
4. Mullis, K.B., and Faloona, F.A. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155, 335-350.
5. Murray, V. (1989). Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Res. 17, 8889.
6. Ruano, G., and Kidd, K. (1991). Coupled amplification and sequencing of genomic DNA. Proc. Natl. Acad. Sci. USA 88, 2815-2819.
7. Rychlik, W. (1993). Selection of primers for PCR. In: Methods in Molecular Biology - PCR Protocols - Current Methods and Applications, B. White, ed. (Totowa, New Jersey, USA: Humana Press), pp. 31-40.
8. Sanger, F., Nicklen, S., and Coulson, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
9. Sarkar, G., and Bolander, M.E. (1995). Semi exponential cycle sequencing. Nucleic Acids Res. 23, 1269-1270.
10. Tabor, S., and Richardson, C.C. (1989). Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymeraseand E. coli polymerase I. Proc. Natl. Acad. Sci. USA 86, 4076-4080.
11. Tabor, S., and Richardson, C.C. (1995). A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy-and dideoxyribonucleotides. Proc. Natl. Acad. Sci. USA 92, 6339-6343.
12. Wiemann, S., Stegemann, J., Grothues, D., Bosch, A., Estivill, X., Schwager, C., Zimmermann, J., Voss, H., and Ansorge, W. (1995). Simultaneous on-line DNA sequencing on both strands with two fluorescent dyes. Analytical Biochemistry 224, 117-121.