Rapid diagnosis of α-thalassemia by melting curve analysis

Journal of Molecular Diagnostics

Volumn 12, Issue 3, 2010, Pages 354-358

  Munkongdee, Thongperm ^a,b   Vattanaviboon, Phantip ^a   Thummarati, Parichut ^a   Sewamart, Paijit ^a   Winichagoon, Pranee ^a   Fucharoen, Suthat ^a   Svasti, Saovaros ^a,c  

^a MAHIDOL UNIVERSITY   (Thailand)

^b MAHIDOL UNIVERSITY   (Thailand)

^c Mahidol University   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GLOBIN; HEMOGLOBIN;

ALPHA THALASSEMIA; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; GENE AMPLIFICATION; GENE DELETION; HETEROZYGOSITY; HOMOZYGOSITY; HUMAN; MAJOR CLINICAL STUDY; MULTIPLEX POLYMERASE CHAIN REACTION; TEMPERATURE SENSITIVITY;

EID: 77951734486     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2010.090136     Document Type: Article

References (14)

1. Weatherall DJ, Clegg JB: The thalassemia syndromes. Malden, MA, Blackwell Science, 2001, pp. 136-150
2. Fucharoen S, Winichagoon P: Hemoglobinopathies in Southeast Asia: molecular biology and clinical medicine. Hemoglobin 1997, 21:299-319 (Pubitemid 27327182)
3. FIL). Br J Haematol 1988, 70:233-238
4. FIL determinants of α-thalassemia in Taiwan. Am J Hematol 1999, 60:80-81
5. Winichagoon P, Higgs DR, Goodbourn SE, Clegg JB, Weatherall DJ, Wasi P: The molecular basis of α-thalassaemia in Thailand. EMBO J 1984, 3:1813-1818
6. Torcharus K, Sriphaisal T, Krutvecho T, Ketupanya A, Vuthiwong C, Suwanasophon C, Noonai A: Prenatal diagnosis of Hb Bart's hydrops fetalis by PCR technique: Pramongkutklao experience. Southeast Asian J Trop Med Public Health 1995, 26(Suppl 1):287-290
7. Chong SS, Boehm CD, Cutting GR, Higgs DR: Simplified multiplex-PCR diagnosis of common Southeast Asian deletional determinants of α-thalassemia. Clin Chem 2000, 46:1692-1695
8. Fucharoen S, Fucharoen G, Fukumaki Y: Simple non-radioactive method for detecting haemoglobin Constant Spring gene. Lancet 1990, 335:1527 (Pubitemid 20224937)
9. Adirojnanon P, Wasi P: Levels of haemoglobin H and proportions of red cells with inclusion bodies in the two types of haemoglobin H disease. Br J Haematol 1980, 46:507-509 (Pubitemid 11226418)
10. Liu J, Yan M, Wang Z, Wang L, Zhou Y, Xiao B: Molecular diagnosis of α-thalassemia by combining real-time PCR with SYBR Green1 and dissociation curve analysis. Transl Res 2006, 148:6-12 (Pubitemid 44302102)
11. Pornprasert S, Phusua A, Suanta S, Saetung R, Sanguansermsri T: Detection of α-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis. Eur J Haematol 2008, 80:510-514 (Pubitemid 351695233)
12. 0-thalassemia using the relative quantitative PCR and the dissociation curve analysis. Clin Lab Haematol 2003, 25:359-365
13. +- thalassemias by determining the ratio of the two α-globin gene copies by oligonucleotide hybridization and melting curve analysis. Clin Chem 2005, 51:1711-1713
14. Ririe KM, Rasmussen RP, Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997, 245:154-160 (Pubitemid 27082244)