The terminal 5′ phosphate and proximate phosphorothioate promote ligation-independent cloning

Protein Science

Volumn 19, Issue 5, 2010, Pages 967-973

  Liu, Xi Peng ^a   Liu, Jian Hua ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

5 phosphate group; Aeropyrum pernix; Cloning efficiency; Ligation independent cloning; Phosphorothioate modified primer

Indexed keywords

5' PHOSPHATE; DNA POLYMERASE; EXONUCLEASE; PHOSPHATE; PHOSPHOROTHIOIC ACID; UNCLASSIFIED DRUG; OLIGONUCLEOTIDE PHOSPHOROTHIOATE;

ARTICLE; DNA REPAIR; ESCHERICHIA COLI; HYBRIDIZATION; IN VIVO STUDY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REACTION TIME; AEROPYRUM; BACTERIAL GENE; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY;

AEROPYRUM PERNIX; ESCHERICHIA COLI;

AEROPYRUM; CLONING, MOLECULAR; EXONUCLEASES; GENES, BACTERIAL; PHOSPHOROTHIOATE OLIGONUCLEOTIDES;

EID: 77951574871     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.374     Document Type: Article

References (18)

1. Scharf SJ, Horn GT, Erlich HA (1986) Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076-1078.
2. Costa GL, Grafsky A, Weiner MP (1994) Cloning and analysis of PCR-generated DNA fragments. PCR Methods Appl 3:338-345. (Pubitemid 24212001)
3. Marchuk D, Drumm M, Saulino A, Collins FS (1991) Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res 19:1154.
4. Kaluz S, Flint APF (1994) Ligation-independent cloning of PCR products with primers containing nonbase residues. Nucleic Acids Res 22:4845. (Pubitemid 24354825)
5. Hsiao K (1993) Exonuclease III induced ligase-free directional subcloning of PCR products. Nucleic Acids Res 21:5528-5529.
6. Aslanidis C, de Jong PJ (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res 18:6069-6074. (Pubitemid 20364668)
7. Yang YS, Watson WJ, Tucker PW, Capra JD (1993) Construction of recombinant DNA by exonuclease recession. Nucleic Acids Res 21:1889-1893.
8. Tseng H (1999) DNA cloning without restriction enzyme and ligase. Biotechniques 27:1240-1244.
9. Nisson PE, Rashtchian A, Watkins PC (1991) Rapid and efficient cloning of Alu-PCR products using uracil DNA glycosylase. PCR Methods Appl 1:120-123.
10. Donahue WF, Turczyk BM, Jarrell KA (2002) Rapid gene cloning using terminator primers and modular vectors. Nucleic Acids Res 30:e95.
11. Coljee VW, Murray HL, Donahue WF, Jarrell KA (2000) Seamless gene engineering using RNA- and DNA-overhang cloning. Nat Biotechnol 18:789-791.
12. Tillett D, Neilan BA (1999) Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites. Nucleic Acids Res 27:e26.
13. van den Ent F, Löwe J (2006) RF cloning: a restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods 67:67-74.
14. Zhang Y, Muyrers JP, Testa G, Stewart AF (2000) DNA cloning by homologous recombination in Escherichia coli. Nat Biotechnol 18:1314-1317.
15. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci USA 97:5978-5983 (Pubitemid 30367509)
16. Iizasa E, Nagano Y (2006) Highly efficient yeast-based in vivo DNA cloning of multiple DNA fragments and the simultaneous construction of yeast/Escherichia coli shuttle vectors. Biotechniques 40:79-83. (Pubitemid 46534942)
17. Nikiforov TT, Rendle RB, Kotewicz ML, Rogers YH (1994) The use of phosphorothioate primers and exonuclease hydrolysis for the preparation of single-stranded PCR products and their detection by solid-phase hybridization. PCR Methods Appl 3:285-291. (Pubitemid 24126356)
18. Zhang W, Evans DH (1995) DNA strand transfer catalyzed by the 5′-3′ exonuclease domain of Escherichia coli DNA polymerase I. Nucleic Acids Res 23:4620-4627. (Pubitemid 126003263)