Site-directed mutagenesis and structural modeling of Coq10p indicate the presence of a tunnel for coenzyme Q6 binding

FEBS Letters

Volumn 584, Issue 8, 2010, Pages 1609-1614

  Busso, Cleverson ^a   Bleicher, Lucas ^a   Ferreira Júnior, José Ribamar ^a   Barros, Mario H ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Coenzyme Q; COQ10; Mitochondrion

Indexed keywords

BINDING PROTEIN; COQ10P PROTEIN; CYTOCHROME C; HYDROGEN PEROXIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UBIQUINONE; UBIQUINONE 6; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; MITOCHONDRIAL ENERGY TRANSFER; MITOCHONDRIAL RESPIRATION; MOLECULAR MODEL; NONHUMAN; OXYGEN CONSUMPTION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; SPHEROPLAST;

EID: 77951299311     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.03.024     Document Type: Article

References (21)

1. Barros M.H., Johnson A., Gin P., Marbois B.N., Clarke C.F., Tzagoloff A. The Saccharomyces cerevisiae COQ10 gene encodes a START domain protein required for function of coenzyme Q in respiration. J. Biol. Chem. 2005, 280:42627-42635.
2. Bateman A., Birney E., Cerruti L., Durbin R., Etwiller L., Eddy S.R., Griffiths-Jones S., Howe K.L., Marshall M., Sonnhammer E.L. The Pfam protein families database. Nucleic Acids Res. 2002, 30:276-280.
3. Tran U.C., Clarke C.F. Endogenous synthesis of coenzyme Q in eukaryotes. Mitochondrion 2007, 7(Suppl. 1):S62-S71.
4. Tzagoloff A., Dieckmann C.L. PET genes of Saccharomyces cerevisiae. Microbiol. Rev. 1990, 54:211-225.
5. Tzagoloff A., Akai A., Needleman R.B. Assembly of the mitochondrial membrane system. Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes. J. Biol. Chem. 1975, 250:8228-8235.
6. Cui T.Z., Kawamukai M. Coq10, a mitochondrial coenzyme Q binding protein, is required for proper respiration in Schizosaccharomyces pombe. FEBS J. 2009, 276:748-759.
7. Shen Y., Goldsmith-Fischman S., Atreya H.S., Acton T., Ma L., Xiao R., Honig B., Montelione G.T., Szyperski T. NMR structure of the 18 kDa protein CC1736 from Caulobacter crescentus identifies a member of the START domain superfamily and suggests residues mediating substrate specificity. Proteins 2005, 58:747-750.
8. Ponting C.P., Aravind L. START: a lipid-binding domain in StAR, HD-ZIP and signalling proteins. Trends Biochem. Sci. 1999, 24:130-132.
9. Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 1986, 2:163-167.
10. Herrmann J.M., Foelsch H., Neupert W., Stuart R.A. Isolation of yeast mitochondria and study of mitochondrial protein translation. Cell Biology: A Laboratory Handbook 1994, Vol. I:538-544. Academic Press, San Diego, CA.
11. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25:3389-3402.
12. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22:4673-4680.
13. Lockless S.W., Ranganathan R. Evolutionarily conserved pathways of energetic connectivity in protein families. Science 1999, 286:295-299.
14. Bachega J.F., Navarro M.V., Bleicher L., Bortoleto-Bugs R.K., Dive D., Hoffmann P., Viscogliosi E., Garratt R.C. Systematic structural studies of iron superoxide dismutases from human parasites and a statistical coupling analysis of metal binding specificity. Proteins 2009, 77:26-37.
15. Combet C., Jambon M., Deleage G., Geourjon C. Geno3D: automatic comparative molecular modelling of protein. Bioinformatics 2002, 18:213-214.
16. Zhang Y. I-TASSER server for protein 3D structure prediction. BMC Bioinf. 2008, 9:40.
17. DeLano W.L. The PyMol Molecular Graphics System 2002, DeLano Scientific, San Carlos, CA.
18. Romanowski M.J., Soccio R.E., Breslow J.L., Burley S.K. Crystal structure of the Mus musculus cholesterol-regulated START protein 4 (StarD4) containing a StAR-related lipid transfer domain. Proc. Natl. Acad. Sci. USA 2002, 99:6949-6954.
19. Boveris A., Chance B. The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochem. J. 1973, 134:707-716.
20. Dröse S., Brandt U. The mechanism of mitochondrial superoxide production by the cytochrome bc1 complex. J. Biol. Chem. 2008, 283:21649-21654.
21. Lin D., Sugawara T., Strauss J.F., Clark B.J., Stocco D.M., Saenger P., Rogol A., Miller W.L. Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis. Science 1995, 267:1828-1831.