Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary electrophoresis

Clinical Biochemistry

Volumn 43, Issue 7-8, 2010, Pages 697-699

  Jiraskova, Alena ^a   Lenicek, Martin ^a   Vitek, Libor ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Genotyping; Heme oxygenase; Methods; Microsatellite variations; UGT1A1

Indexed keywords

FLUORESCENT DYE; GENOMIC DNA; GLUCURONOSYLTRANSFERASE 1A1; HEME OXYGENASE 1;

ALLELE; AMPLICON; ARTICLE; CAPILLARY ELECTROPHORESIS; GENETIC ANALYSIS; GENETIC VARIABILITY; GENOTYPE; HUMAN; LEUKOCYTE; MICROSATELLITE MARKER; MULTICOLORED CAPILLARY ELECTROPHORESIS; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION;

ELECTROPHORESIS, CAPILLARY; GENOTYPE; GLUCURONOSYLTRANSFERASE; HEME OXYGENASE-1; HUMANS; MICROSATELLITE REPEATS; POLYMORPHISM, SINGLE NUCLEOTIDE; REPRODUCIBILITY OF RESULTS;

EID: 77951205418     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2010.01.006     Document Type: Article

References (7)

1. Vítek L., Schwertner H.A. The heme catabolic pathway and its protective effects on oxidative stress-mediated diseases. Adv. Clin. Chem. 2007, 43:1-57.
2. Schwertner H.A., Vítek L. Gilbert syndrome, UGT1A1*28 allele, and cardiovascular disease risk: possible protective effects and therapeutic applications of bilirubin. Atherosclerosis 2008, 198:1-11.
3. Exner M., Minar E., Wagner O., Schillinger M. The role of heme oxygenase-1 promoter polymorphisms in human disease. Free Radic. Biol. Med. 2004, 37:1097-1104.
4. Baudhuin L.M., Highsmith W.E., Skierka J., Holtegaard L., Moore B.E., O'Kane D.J. Comparison of three methods for genotyping the UGT1A1 (TA)n repeat polymorphism. Clin. Biochem. 2007, 40:710-717.
5. Mansfield E.S., Vainer M., Enad S., Barker D.L., Harris D., Rappaport E., et al. Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis. Genome Res. 1996, 6:893-903.
6. Delmotte F., Leterme N., Simon J.C. Microsatellite allele sizing: difference between automated capillary electrophoresis and manual technique. BioTechniques 2001, 31:810,814-816,818.
7. Haberl M., Tautz D. Comparative allele sizing can produce inaccurate allele size differences of microsatellites. Mol. Ecol. 1999, 8:1347-1350.