A semisynthesis platform for investigating structure?function relationships in the N-terminal domain of the anthrax lethal factor

ACS Chemical Biology

Volumn 5, Issue 4, 2010, Pages 359-364

  Pentelute, Brad L ^a   Barker, Adam P ^a   Janowiak, Blythe E ^a   Kent, Stephen B H ^b   Collier, R John ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; GUANIDINE; HYBRID PROTEIN; MESNA; SUMO PROTEIN; TRIFLUOROACETIC ACID;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LETHAL GENE; PHOSPHOLIPID BILAYER; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; VOLTAGE CLAMP;

AMINO ACID SEQUENCE; ANTHRAX; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACTERIAL TOXINS; IONS; LIPID BILAYERS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 77951111396     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb100003r     Document Type: Article

References (24)

1. Young, J. A. and Collier, R. J. (2007) Anthrax toxin: receptor binding, internalization, pore formation, and translocation Annu. Rev. Biochem. 76, 243-265
2. Bradley, K. A., Mogridge, J., Mourez, M., Collier, R. J., and Young, J. A. (2001) Identification of the cellular receptor for anthrax toxin Nature 414, 225-229
3. Scobie, H. M., Rainey, G. J., Bradley, K. A., and Young, J. A. (2003) Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor Proc. Natl. Acad. Sci. U.S.A. 100, 5170-5174
4. Molloy, S. S., Bresnahan, P. A., Leppla, S. H., Klimpel, K. R., and Thomas, G. (1992) Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen J. Biol. Chem. 267, 16396-16402
5. Klimpel, K. R., Molloy, S. S., Thomas, G., and Leppla, S. H. (1992) Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin Proc. Natl. Acad. Sci. U.S.A. 89, 10277-10281
6. Milne, J. C., Furlong, D., Hanna, P. C., Wall, J. S., and Collier, R. J. (1994) Anthrax protective antigen forms oligomers during intoxication of mammalian cells J. Biol. Chem. 269, 20607-20612
7. Kintzer, A. F., Thoren, K. L., Sterling, H. J., Dong, K. C., Feld, G. K., Tang, I. I., Zhang, T. T., Williams, E. R., Berger, J. M., and Krantz, B. A. (2009) The protective antigen component of anthrax toxin forms functional octameric complexes J. Mol. Biol. 392, 614-629
8. Abrami, L., Liu, S., Cosson, P., Leppla, S. H., and van der Goot, F. G. (2003) Anthrax toxin triggers endocytosis of its receptor via a lipid raft-mediated clathrin-dependent process J. Cell Biol. 160, 321-328
9. Abrami, L., Lindsay, M., Parton, R. G., Leppla, S. H., and van der Goot, F. G. (2004) Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway J. Cell Biol. 166, 645-651
10. Zhang, S., Finkelstein, A., and Collier, R. J. (2004) Evidence that translocation of anthrax toxin's lethal factor is initiated by entry of its N terminus into the protective antigen channel Proc. Natl. Acad. Sci. U.S.A. 101, 16756-16761
11. Muir, T. W. (2008) Studying protein structure and function using semisynthesis Biopolymers 90, 743-750
12. Dawson, P. E., Muir, T. W., Clark-Lewis, I., and Kent, S. B. (1994) Synthesis of proteins by native chemical ligation Science 266, 776-779
13. Zhang, S., Udho, E., Wu, Z., Collier, R. J., and Finkelstein, A. (2004) Protein translocation through anthrax toxin channels formed in planar lipid bilayers Biophys. J. 87, 3842-3849
14. Krantz, B. A., Finkelstein, A., and Collier, R. J. (2006) Protein translocation through the anthrax toxin transmembrane pore is driven by a proton gradient J. Mol. Biol. 355, 968-979
15. Blanco-Canosa, J. B. and Dawson, P. E. (2008) An efficient Fmoc-SPPS approach for the generation of thioester peptide precursors for use in native chemical ligation Angew. Chem., Int. Ed. 47, 6851-6855
16. Johnson, E. C. and Kent, S. B. (2006) Insights into the mechanism and catalysis of the native chemical ligation reaction J. Am. Chem. Soc. 128, 6640-6646
17. Macmillan, D. and Arham, L. (2004) Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation J. Am. Chem. Soc. 126, 9530-9531
18. Krantz, B. A., Trivedi, A. D., Cunningham, K., Christensen, K. A., and Collier, R. J. (2004) Acid-induced unfolding of the amino-terminal domains of the lethal and edema factors of anthrax toxin J. Mol. Biol. 344, 739-756
19. Blaustein, R. O., Koehler, T. M., Collier, R. J., and Finkelstein, A. (1989) Anthrax toxin: channel-forming activity of protective antigen in planar phospholipid bilayers Proc. Natl. Acad. Sci. U.S.A. 86, 2209-2213
20. Mueller, P, W. W., Rudin, D. O., and Tien, H. T. (1963) Methods for formation of single biomolecular lipid membranes in aquesous solution J. Phys. Chem. 67, 534-535
21. Krantz, B. A., Melnyk, R. A., Zhang, S., Juris, S. J., Lacy, D. B., Wu, Z., Finkelstein, A., and Collier, R. J. (2005) A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore Science 309, 777-781
22. Schnolzer, M., Alewood, P., Jones, A., Alewood, D., and Kent, S. B. (1992) In situ neutralization in Boc-chemistry solid phase peptide synthesis. Rapid, high yield assembly of difficult sequences Int. J. Pept. Protein Res. 40, 180-193
23. Miller, C. J., Elliott, J. L., and Collier, R. J. (1999) Anthrax protective antigen: prepore-to-pore conversion Biochemistry 38, 10432-10441
24. Katayama, H., Janowiak, B. E., Brzozowski, M., Juryck, J., Falke, S., Gogol, E. P., Collier, R. J., and Fisher, M. T. (2008) GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore Nat. Struct. Mol. Biol. 15, 754-760