Quantitative analysis of EAAT4 promoter activity in neurons and astrocytes of mouse somatic sensory cortex

Neuroscience Letters

Volumn 474, Issue 1, 2010, Pages 42-45

  de Vivo, Luisa ^a   Melone, Marcello ^a   Bucci, Giovanna ^a   Rothstein, Jeffrey D ^b   Conti, Fiorenzo ^a  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Astrocytes; EAAT4; Glutamate transporters; Neocortex; Neurons

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; EXCITATORY AMINO ACID TRANSPORTER 4; GLUTAMIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BACTERIAL ARTIFICIAL CHROMOSOME; GENE EXPRESSION PROFILING; GENE FUNCTION; GENE IDENTIFICATION; GENE LOCATION; MOUSE; NERVE CELL; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PURKINJE CELL; QUANTITATIVE ANALYSIS; REPORTER GENE; SOMATOSENSORY CORTEX;

ANIMALS; ASTROCYTES; EXCITATORY AMINO ACID TRANSPORTER 4; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; MICE; MICE, TRANSGENIC; NEURONS; PROMOTER REGIONS, GENETIC; PURKINJE CELLS; SOMATOSENSORY CORTEX;

EID: 77950960621     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2010.03.003     Document Type: Article

References (30)

1. Bignami A., Dahl A. Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein. J. Comp. Neurol. 1973, 153:27-38.
2. Brasnjo G., Otis T.S. Neuronal glutamate transporters control activation of postsynaptic metabotropic glutamate receptors and influence cerebellar long-term depression. Neuron 2001, 31:607-616.
3. Braun P.E., Sandillon F., Edwards A., Matthieu J.M., Privat A. Immunocytochemical localization by electron microscopy of 2′3′-cyclic nucleotide 3′-phosphodiesterase in developing oligodendrocytes of normal and mutant brain. J. Neurosci. 1988, 8:3057-3066.
4. Conti F., Weinberg R.J. Shaping excitation at glutamatergic synapses. Trends Neurosci. 1999, 22:451-458.
5. Danbolt N.C. Glutamate uptake. Prog. Neurobiol. 2001, 65:1-105.
6. Dehnes Y., Chaudhry F.A., Ullensvang K., Lehre K.P., Storm-Mathisen J., Danbolt N.C. The glutamate transporter EAAT4 in rat cerebellar Purkinje cells: a glutamate-gated chloride channel concentrated near the synapse in parts of the dendritic membrane facing astroglia. J. Neurosci. 1998, 18:3606-3619.
7. de Vivo L., Melone M., Rothstein J.D., Conti F. GLT-1 promoter activity in astrocytes and neurons of mouse hippocampus and somatic sensory cortex. Front. Neuroanat. 2010, 3:31. 10.3389/neuro.05.031.2009.
8. Fairman W.A., Vandenberg R.J., Arriza L., Kavanaugh M.P., Amara S.G. An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature 1995, 375:599-603.
9. Furuta A., Martin L.J., Lin C.L., Dykes-Hoberg M., Rothstein J.D. Cellular and synaptic localization of the neuronal glutamate transporters excitatory amino acid transporter 3 and 4. Neuroscience 1997, 81:1031-1042.
10. Furuta A., Rothstein J.D., Martin L.J. Glutamate transporter protein subtypes are expressed differentially during rat CNS development. J. Neurosci. 1997, 17:8363-8375.
11. Gincel D., Regan M.R., Jin L., Watkins A.M., Bergles D.E., Rothstein J.D. Analysis of cerebellar Purkinje cells using EAAT4 glutamate transporter promoter reporter in mice generated via bacterial artificial chromosome-mediated transgenesis. Exp. Neurol. 2007, 203:205-212.
12. Hu W.H., Walters W.M., Xia X.M., Karmally S.A., Bethea J.R. Neuronal glutamate transporter EAAT4 is expressed in astrocytes. Glia 2003, 44:13-25.
13. Liang J., Takeuchi H., Doi Y., Kawanokuchi J., Sonobe Y., Jin S., Yawata I., Li H., Yasuoka S., Mizuno T., Suzumura A. Excitatory amino acid transporter expression by astrocytes is neuroprotective against microglial excitotoxicity. Brain Res. 2008, 1210:11-19.
14. Lin C.L., Tzingounis A.V., Jin L., Furuta A., Kavanaugh M.P., Rothstein J.D. Molecular cloning and expression of the rat EAAT4 glutamate transporter subtype. Brain Res. Mol. Brain Res. 1998, 63:174-179.
15. Marrs G.S., Green S.H., Dailey M.E. Rapid formation and remodeling of postsynaptic densities in developing dendrites. Nat. Neurosci. 2001, 4:1006-1013.
16. +-dependent glutamate transporter EAAT4 is expressed throughout the rat fore- and midbrain. J. Comp. Neurol. 2008, 511:155-172.
17. Massie A., Vandesande F., Arckens L. Expression of the high-affinity glutamate transporter EAAT4 in mammalian cerebral cortex. NeuroReport 2001, 12:393-397.
18. Mim C., Balani P., Rauen T., Grewer C. The glutamate transporter subtypes EAAT4 and EAATs 1-3 transport glutamate with dramatically different kinetics and voltage dependence but share a common uptake mechanism. J. Gen. Physiol. 2005, 126:571-589.
19. Mullen R.J., Buck C.R., Smith A.M. NeuN a neuronal specific nuclear protein in vertebrates. Development 1992, 116:201-211.
20. Nagao S., Kwak S., Kanazawa I. EAAT4, a glutamate transporter with properties of a chloride channel, is predominantly localized in Purkinje cell dendrites, and forms parasagittal compartments in rat cerebellum. Neuroscience 1997, 78:929-933.
21. Nikkuni O., Takayasu Y., Iino M., Tanaka K., Ozawa S. Facilitated activation of metabotropic glutamate receptors in cerebellar Purkinje cells in glutamate transporter EAAT4-deficient mice. Neurosci. Res. 2007, 59:296-303.
22. Paxinos G., Franklin K.B.J. The Mouse Brain in Stereotaxic Coordinates 2001, Academic Press, San Diego. 2nd ed.
23. Popovich P.G., Wei P., Stokes B.T. Cellular inflammatory response after spinal cord injury in Sprague-Dawley and Lewis rats. J. Comp. Neurol. 1997, 377:443-464.
24. Rakhade S.N., Loeb J.A. Focal reduction of neuronal glutamate transporters in human neocortical epilepsy. Epilepsia 2008, 49:226-236.
25. Regan M.R., Huang Y.H., Kim Y.S., Dykes-Hoberg M.I., Jin L., Watkins A.M., Bergles D.E., Rothstein J.D. Variations in promoter activity reveal a differential expression and physiology of glutamate transporters by glia in the developing and mature CNS. J. Neurosci. 2007, 27:6607-6619.
26. Takayasu Y., Iino M., Kakegawa W., Maeno H., Watase K., Wada K., Yanagihara D., Miyazaki T., Komine O., Watanabe M., Tanaka K., Ozawa S. Differential roles of glial and neuronal glutamate transporters in Purkinje cell synapses. J. Neurosci. 2005, 25:8788-8793.
27. Tanaka J., Ichikawa R., Watanabe M., Tanaka K., Inoue Y. Extra-junctional localization of glutamate transporter EAAT4 at excitatory Purkinje cell synapses. NeuroReport 1997, 8:2461-2464.
28. Wadiche J.I., Jahr C.E. Patterned expression of Purkinje cell glutamate transporters controls synaptic plasticity. Nat. Neurosci. 2005, 8:1329-1334.
29. Ward M.M., Jobling A.I., Puthussery T., Foster L.E., Fletcher E.L. Localization and expression of the glutamate transporter, excitatory amino acid transporter 4, within astrocytes of the rat retina. Cell Tissue Res. 2004, 315:305-310.
30. Yi J.H, Herrero R., Chen G., Hazell A.S. Glutamate transporter EAAT4 is increased in hippocampal astrocytes following lateral fluid-percussion injury in the rat. Brain Res. 2007, 1154:200-205.