Isolation and proteomic analysis of the halotolerant alga Dunaliella salina flagella using shotgun strategy

Molecular Biology Reports

Volumn 37, Issue 2, 2010, Pages 711-716

  Jia, Yanlong ^a,b   Xue, Lexun ^a,b   Li, Jie ^b   Liu, Hongtao ^b  

^a THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

^b ZHENGZHOU UNIVERSITY   (China)

Author keywords

Dunaliella salina; Flagella; Proteomics; Shotgun

Indexed keywords

ALGAL PROTEIN; ALPHA TUBULIN; ANAPHASE PROMOTING COMPLEX; BETA TUBULIN; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE; DYNEIN ADENOSINE TRIPHOSPHATASE; ENOLASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK PROTEIN 70; KINESIN K39; KINESIN LIKE PROTEIN 1; MITOGEN ACTIVATED PROTEIN KINASE; NUCLEIC ACID BINDING PROTEIN; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; UNCLASSIFIED DRUG; HALOGEN; PROTEOME;

ARTICLE; CELL DIVISION; DUNALIELLA SALINA; FLAGELLUM; GREEN ALGA; HALOPHILE; METABOLISM; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEOMICS; SHOTGUN PROTEOMIC ANALYSIS; SIGNAL TRANSDUCTION; ACCLIMATIZATION; CHEMISTRY; DRUG EFFECT; ISOLATION AND PURIFICATION; METHODOLOGY; PHYSIOLOGY; PROTEIN DATABASE; SALT TOLERANCE;

ALGAE; CHLOROPHYTA; DUNALIELLA SALINA; EUKARYOTA;

ACCLIMATIZATION; ALGAE, GREEN; ALGAL PROTEINS; DATABASES, PROTEIN; FLAGELLA; HALOGENS; PROTEOME; PROTEOMICS; SALT-TOLERANCE;

EID: 77950908818     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9563-x     Document Type: Article

References (30)

1. Eley L, Yates LM, Goodship JA (2005) Cilia and disease. Curr Opin Genet Dev 15:308-314. doi:10.1016/j.gde.2005.04.008 (Pubitemid 40726055)
2. Sloboda RD, Rosenbaum JL (2007) Making sense of cilia and flagella. J Cell Biol 179:575-582. doi:10.1083/jcb.200709039 (Pubitemid 350146243)
3. Vogel G (2005) News focus: betting on cilia. Science 310:216-218. doi:10.1126/science.310.5746.216 (Pubitemid 41457168)
4. Michaud EJ, Yoder BK (2006) The primary cilium in cell signaling and cancer. Cancer Res 66:6463-6467. doi:10.1158/0008- 5472.CAN-06-0462
5. Pugacheva EN, Jablonski SA, Hartman TR, Henske EP, Golemis EA (2007) HEF1-dependent Aurora A activation induces disassembly of the primary cilium. Cell 129:1351-1363. doi:10.1016/ j.cell.2007.04.035
6. Pedersen LB, Veland IR, Schroder JM, Christensen ST (2008) Assembly of primary cilia. Dev Dyn 237:1993-2006. doi: 10.1002/dvdy.21521
7. Snell WJ, Pan J, Wang Q (2004) Cilia and flagella revealed: from flagellar assembly in Chlamydomonas to human obesity disorders. Cell 117:693-697. doi:10.1016/j.cell.2004.05.019 (Pubitemid 38748886)
8. Vismara R, Verni F, Barsanti L, Evangelista V, Gualtieri P (2004) A short flagella mutant of Dunaliella salina (Chlorophyta, Chlorophyceae). Micron 35:337-344. doi:10.1016/j.micron.2004. 01.001
9. Xie H, Xu P, Jia Y, Li J, Lu Y, Xue L (2007) Clonging and heterologous expression of nitrate reductase genes from Dunliella salina. J Appl Phycol 19:497-504. doi:10.1007/s10811-007-91 62-y
10. Katz A, Waridel P, Shevchenko A, Pick U (2007) Salt-induced changes in the plasma membrane proteome of the halotolerant alga Dunaliella salina as revealed by blue native gel electrophoresis and nano-LC-MS/MS analysis. Mol Cell Proteomics 6:1459-1472. doi:10.1074/mcp.M700002-MCP200 (Pubitemid 47506160)
11. Liska AJ, Shevchenko A, Pick U, Katz A (2004) Enhanced photosynthesis and redox energy production contribute to salinity tolerance in Dunaliella as revealed by homology-based proteomics. Plant Physiol 136:2806-2817. doi:10.1104/pp.104.039438
12. McDonald WH, Yates JR (2003) Shotgun proteomics: integrating technologies to answer biological questions. Curr Opin Mol Ther 5:302-309
13. Swanson SK, Washburn MP (2005) The continuing evolution of shotgun proteomics. Drug Discov Today 10:719-725. doi: 10.1016/S1359-6446(05)03450-1 (Pubitemid 40724554)
14. Ben-Amotz A, Avron M (1990) The biotechnology of cultivating the halotolerant alga Dunaliella. Trends Biotechnol 8:121-126. doi:10.1016/0167- 7799(90)90152-N
15. Witman GB (1986) Isolation of Chlamydomonas flagella and flagellar axonemes. Methods Enzymol 134:280-290. doi: 10.1016/0076-6879(86)34096-5
16. Gu H (2002) A new silver-plating method for staining flagella. Chin J Microbiol Immunol 22:106-111
17. Hirano M, Rakwal R, Shibato J, Agrawal GK, Jwa NS, Iwahashi H, Masuo Y (2006) New protein extraction/solubilization protocol for gel-based proteomics of rat (female) whole brain and brain regions. Mol Cells 22:119-125
18. Natarajan S, Xu C, Caperna TJ, Garrett WM (2005) Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins. Anal Biochem 342:214-220. doi: 10.1016/j.ab.2005.04.046 (Pubitemid 40884569)
19. Dai J, Shieh CH, Sheng QH, Zhou H, Zeng R (2005) Proteomic analysis with integrated multiple dimensional liquid chromatography/ mass spectrometry based on elution of ion exchange column using pH steps. Anal Chem 77:5793-5799. doi:10.1021/ ac050251w
20. Washburn MP, Wolters D, Yates JRIII (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 19:242-247. doi:10.1038/85686 (Pubitemid 32220565)
21. Pazour GJ, Agrin N, Leszyk J, Witman GB (2005) Proteomic analysis of a eukaryotic cilium. J Cell Biol 170:103-113. doi: 10.1083/jcb.200504008 (Pubitemid 40994100)
22. Gherman A, Davis EE, Katsanis N (2006) The ciliary proteome database: an integrated community resource for the genetic and functional dissection of cilia. Nat Genet 38:961-962. doi: 10.1038/ng0906-961 (Pubitemid 44325911)
23. Oren A (2005) A hundred years of Dunaliella research: 1905- 2005. Saline Syst 1:2-16. doi:10.1186/1746-1448-1-2
24. Scholey JM (2008) Intraflagellar transport motors in cilia: moving along the cell's antenna. J Cell Biol 180:23-29. doi:10.1083/ jcb.200709133
25. Pazour GJ, Witman GB (2003) The vertebrate primary cilium is a sensory organelle. Curr Opin Cell Biol 15:105-110. doi:10.1016/ S0955-0674(02)00012-1
26. Pan J, Snell W (2007) The primary cilium: keeper of the key to cell division. Cell 129:1255-1257. doi:10.1016/j.cell.2007.06. 018
27. Quarmby LM, Parker JDK (2005) Cilia and the cell cycle? J Cell Biol 169:707-710. doi:10.1083/jcb.200503053 (Pubitemid 41002875)
28. Klein U (1986) Compartmentation of glycolysis and of the oxidative pentose-phosphate pathway in Chlamydomonas reinhardtii. Planta 167:81-86. doi:10.1007/BF00446372
29. Miki K, Qu W, Goulding EH, Willis WD, Bunch DO, Strader LF, Perreault SD, Eddy EM, O'Brien DA (2004) Glyceraldehyde 3- phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proc Natl Acad Sci USA 101:16501-16506. doi:10.1073/pnas.0407708101 (Pubitemid 39557742)
30. Lee CL, Hsiao HH, Lin CW, Wu SP, Huang SY, Wu CY, Wang AH, Khoo KH (2003) Strategic shotgun proteomics approach for efficient construction of an expression map of targeted protein families in hepatoma cell lines. Proteomics 3:2472-2486. doi: 10.1002/pmic.200300586 (Pubitemid 38010124)