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Volumn 131, Issue 8, 2009, Pages 2766-2767

Hydrophilic fluorescent nanogel thermometer for intracellular thermometry

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL PROCESS; HIGHER TEMPERATURES; NANOGELS; TEMPERATURE RESOLUTION; TEMPERATURE VARIATION;

EID: 77950859284     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja807714j     Document Type: Article
Times cited : (478)

References (29)
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    • 77951288991 scopus 로고    scopus 로고
    • This was confirmed by elemental analysis of sulfur. See the SupportingInformation
    • This was confirmed by elemental analysis of sulfur. See the SupportingInformation.
  • 20
    • 77951289803 scopus 로고    scopus 로고
    • A transmission electron microscopy image indicated that the averagediameter of 1 was 51.2 ± 10.5 nm (mean ± s.d.) in the dried state.Dynamic light scattering and fluorescence measurements on 1 dispersed in waterdemonstrated a reversible heat-induced size variation and consequentfluorescence switching. See Figures S1-S3 in the Supporting Information.
    • A transmission electron microscopy image indicated that the averagediameter of 1 was 51.2 ± 10.5 nm (mean ± s.d.) in the dried state.Dynamic light scattering and fluorescence measurements on 1 dispersed in waterdemonstrated a reversible heat-induced size variation and consequentfluorescence switching. See Figures S1-S3 in the Supporting Information.
  • 21
    • 0035860994 scopus 로고    scopus 로고
    • A related temperature-jump investigation revealed that heat-inducedshrinking of a polyNIPAM nanogel occurs on a microsecond time scale. See: Wang.J, Gan, D, Lyon, L. A, El-Saved, M. A. J.Am. Chem. Soc. 2001,123, 11284-11289
    • A related temperature-jump investigation revealed that heat-inducedshrinking of a polyNIPAM nanogel occurs on a microsecond time scale. See: Wang.J.; Gan, D.; Lyon, L. A.; El-Saved, M. A. J.Am. Chem. Soc. 2001,123, 11284-11289.
  • 22
    • 77951287654 scopus 로고    scopus 로고
    • Lodish, H. F.; Berk, A.; Matsudaira, P.; Kaiser, C. A.; Krieger, M.;Scott, M. P.; Zipursky, S. L.; Damell, J. Molecular Cell Biology, 5thed.; W. H. Freeman: New York, 2003; pp 253 and 261.
    • Lodish, H. F.; Berk, A.; Matsudaira, P.; Kaiser, C. A.; Krieger, M.;Scott, M. P.; Zipursky, S. L.; Damell, J. Molecular Cell Biology, 5thed.; W. H. Freeman: New York, 2003; pp 253 and 261.
  • 24
    • 77951275468 scopus 로고    scopus 로고
    • For detailed data, see the Supporting Information
    • For detailed data, see the Supporting Information.
  • 25
    • 77951288490 scopus 로고    scopus 로고
    • The response curve of 1 in the COS7 cells (2.6-fold fluorescenceenhancement over the range 27-33°C) was not identical to that in the 150 mMKCl solution (5.3-fold over the range 30-40°C). This gap is possibly due tocomplexity of intracellular environments, and therefore, a calibration curveshould be obtained for each cell species before intracellular thermometry.
    • The response curve of 1 in the COS7 cells (2.6-fold fluorescenceenhancement over the range 27-33°C) was not identical to that in the 150 mMKCl solution (5.3-fold over the range 30-40°C). This gap is possibly due tocomplexity of intracellular environments, and therefore, a calibration curveshould be obtained for each cell species before intracellular thermometry.
  • 26
    • 77951266307 scopus 로고    scopus 로고
    • The estimated contribution of autofluorescence to the ordinate was<0.016
    • The estimated contribution of autofluorescence to the ordinate was<0.016.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.