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19
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77951288991
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This was confirmed by elemental analysis of sulfur. See the SupportingInformation
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This was confirmed by elemental analysis of sulfur. See the SupportingInformation.
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20
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77951289803
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A transmission electron microscopy image indicated that the averagediameter of 1 was 51.2 ± 10.5 nm (mean ± s.d.) in the dried state.Dynamic light scattering and fluorescence measurements on 1 dispersed in waterdemonstrated a reversible heat-induced size variation and consequentfluorescence switching. See Figures S1-S3 in the Supporting Information.
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A transmission electron microscopy image indicated that the averagediameter of 1 was 51.2 ± 10.5 nm (mean ± s.d.) in the dried state.Dynamic light scattering and fluorescence measurements on 1 dispersed in waterdemonstrated a reversible heat-induced size variation and consequentfluorescence switching. See Figures S1-S3 in the Supporting Information.
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21
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0035860994
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A related temperature-jump investigation revealed that heat-inducedshrinking of a polyNIPAM nanogel occurs on a microsecond time scale. See: Wang.J, Gan, D, Lyon, L. A, El-Saved, M. A. J.Am. Chem. Soc. 2001,123, 11284-11289
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A related temperature-jump investigation revealed that heat-inducedshrinking of a polyNIPAM nanogel occurs on a microsecond time scale. See: Wang.J.; Gan, D.; Lyon, L. A.; El-Saved, M. A. J.Am. Chem. Soc. 2001,123, 11284-11289.
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77951287654
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Lodish, H. F.; Berk, A.; Matsudaira, P.; Kaiser, C. A.; Krieger, M.;Scott, M. P.; Zipursky, S. L.; Damell, J. Molecular Cell Biology, 5thed.; W. H. Freeman: New York, 2003; pp 253 and 261.
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24
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77951275468
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For detailed data, see the Supporting Information
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For detailed data, see the Supporting Information.
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25
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77951288490
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The response curve of 1 in the COS7 cells (2.6-fold fluorescenceenhancement over the range 27-33°C) was not identical to that in the 150 mMKCl solution (5.3-fold over the range 30-40°C). This gap is possibly due tocomplexity of intracellular environments, and therefore, a calibration curveshould be obtained for each cell species before intracellular thermometry.
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The response curve of 1 in the COS7 cells (2.6-fold fluorescenceenhancement over the range 27-33°C) was not identical to that in the 150 mMKCl solution (5.3-fold over the range 30-40°C). This gap is possibly due tocomplexity of intracellular environments, and therefore, a calibration curveshould be obtained for each cell species before intracellular thermometry.
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26
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77951266307
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The estimated contribution of autofluorescence to the ordinate was<0.016
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The estimated contribution of autofluorescence to the ordinate was<0.016.
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27
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0032025271
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