Transcriptional analysis of virulence-related genes in enterococci from distinct origins

Journal of Applied Microbiology

Volumn 108, Issue 5, 2010, Pages 1563-1575

  Carlos, A R ^a   Semedo Lemsaddek, T ^a   Barreto Crespo, M T ^b   Tenreiro, R ^a  

^a UNIVERSITY OF LISBON   (Portugal)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

Author keywords

Clinical strains; Enterococci; Food strains; RT PCR; Virulence related genes

Indexed keywords

STRAIN;

CLINICAL STRAINS; ENTEROCOCCUS; EXPRESSION PROFILE; FOOD FERMENTATION; FOOD STRAIN; HUMAN PATHOGENS; PROBIOTICS; RT-PCR; TRANSCRIPTIONAL ANALYSIS; VIRULENCE-RELATED GENE;

GENE EXPRESSION;

BACTERIUM; GENE EXPRESSION; GENETIC ANALYSIS; GROWTH; IDENTIFICATION METHOD; MICROBIOLOGY; PATHOGEN; POLYMERASE CHAIN REACTION; VIRULENCE;

AMPLICON; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; COLONY FORMING UNIT; CONTROLLED STUDY; DNA EXTRACTION; DOWN REGULATION; ENTEROCOCCUS; GENE AMPLIFICATION; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; UPREGULATION;

EID: 77950658716     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04551.x     Document Type: Article

References (52)

1. Agerholm-Larsen, L., Bell, M.L., Grunwald, G.K. Astrup, A. (2000) The effect of a probiotic milk product on plasma cholesterol: a meta-analysis of short-term intervention studies. Eur J Clin Nutr 49, 346 352.
2. Alves, P.I., Martins, M.P., Semedo, T., Figueiredo-Marques, J.J., Tenreiro, R. Barreto-Crespo, M.T. (2004) Comparison of phenotypic and genotypic taxonomic methods for the identification of dairy enterococci. Antonie Van Leeuwenhoek 85, 237 252.
3. Andrighetto, C., Knijff, E., Lombardi, A., Torrioni, S., Vancanneyt, M., Kersters, K., Swings, J. Dellaglio, F. (2001) Phenotypic and genetic diversity of enterococci isolated from Italian cheeses. J Dairy Res 68, 303 316.
4. Bellomo, G., Mangiagle, A., Nicastro, L. Frigerio, G. (1980) A controlled double-blind study of SF68 strain as a new biological preparation for the treatment of diarrhoea in pediatrics. Curr Ther Res 28, 927 934.
5. Callegan, M.C., Booth, M.C., Jett, B.D. Gilmore, M.S. (1999) Pathogenesis of gram-positive bacterial endophthalmitis. Infect Immun 67, 3348 3356.
6. Clewell, D.B. (1990) Movable genetic elements and antibiotic resistance in enterococci. Eur J Clin Microbiol Infect Dis 9, 90 102.
7. Day, A.M., Cove, J.H. Phillips-Jones, M.K. (2003) Cytolysin gene expression in Enterococcus faecalis is regulated in response to aerobiosis conditions. Mol Genet Genomics 269, 31 39.
8. Desmasures, N., Bazin, F. Gueguen, M. (1997) Microbiological composition of raw milk from selected farms in the Camembert region of Normandy. J Appl Microbiol 83, 53 58.
9. Eaton, T.J. Gasson, M.J. (2001) Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 67, 1628 1635.
10. Eaton, T.J. Gasson, M.J. (2002) A variant enterococcal surface protein Esp(fm) in Enterococcus faecium; distribution among food, commensal, medical, and environmental isolates. FEMS Microbiol Lett 216, 269 275.
11. Eliopoulos, G.M., Wennersten, C., Zighelboim-Daum, S., Reiszner, E., Goldmann, D. Moellering, R.C. Jr. (1988) High-level resistance to gentamicin in clinical isolates of Streptococcus (Enterococcus) faecium. Antimicrob Agents Chemother 32, 1528 1532.
12. Franz, C.M., Muscholl-Silberhorn, A.B., Yousif, N.M., Vancanneyt, M., Swings, J. Holzapfel, W.H. (2001) Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food. Appl Environ Microbiol 67, 4385 4389.
13. Galli, D. Wirth, R. (1991) Comparative analysis of Enterococcus faecalis sex pheromone plasmids identifies a single homologous DNA region which codes for aggregation substance. J Bacteriol 173, 3029 3033.
14. Gilmore, M.S., Segarra, R.A., Booth, M.C., Bogie, C.P., Hall, L.R. Clewell, D.B. (1994) Genetic structure of the Enterococcus faecalis plasmid pAD1-encoded cytolytic toxin system and its relationship to lantibiotic determinants. J Bacteriol 176, 7335 7344.
15. Guzman, C.A., Pruzzo, C., LiPira, G. Calegari, L. (1989) Role of adherence in pathogenesis of Enterococcus faecalis urinary tract infection and endocarditis. Infect Immun 57, 1834 1838.
16. Guzman, C.A., Pruzzo, C., Plate, M., Guardati, M.C. Calegari, L. (1991) Serum dependent expression of Enterococcus faecalis adhesins involved in the colonization of heart cells. Microb Pathog 11, 399 409.
17. Haas, W. Gilmore, M.S. (1999) Molecular nature of a novel bacterial toxin: the cytolysin of Enterococcus faecalis. Med Microbiol Immunol 187, 183 190.
18. Hew, C.M., Korakli, M. Vogel, R.F. (2007) Expression of virulence-related genes by Enterococcus faecalis in response to different environments. Syst Appl Microbiol 30, 257 267.
19. Higashide, T., Takahashi, M., Kobayashi, A., Ohkubo, S., Sakurai, M., Shirao, Y., Tamura, T. Sugiyama, K. (2005) Endophthalmitis caused by Enterococcus mundtii. J Clin Microbiol 43, 1475 1476.
20. Huycke, M.M., Spiegel, C.A. Gilmore, M.S. (1991) Bacteremia caused by hemolytic, high-level gentamicin-resistant Enterococcus faecalis. Antimicrob Agents Chemother 35, 1626 1634.
21. Iaria, C., Stassi, G., Costa, G.B., Di Leo, R., Toscano, A. Cascio, A. (2005) Enterococcal meningitis caused by Enterococcus casseliflavus. First case report. BMC Infect Dis 5, 3.
22. Ike, Y., Hashimoto, H. Clewell, D.B. (1984) Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice. Infect Immun 45, 528 530.
23. Jett, B.D., Jensen, H.G., Nordquist, R.E. Gilmore, M.S. (1992) Contribution of the pADi-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis. Infect Immun 60, 2445 2452.
24. Jett, B.D., Huycke, M.M. Gilmore, M.S. (1994) Virulence of enterococci. Clin Microbiol Rev 7, 462 478.
25. Jett, B.D., Jensen, H.G., Atkuri, R.V. Gilmore, M.S. (1995) Evaluation of therapeutic measures for treating endophthalmitis caused by isogenic toxin-producing and toxin-nonproducing Enterococcus faecalis strains. Invest Ophthalmol Vis Sci 36, 9 15.
26. Kreft, B., Marre, R., Schramm, U. Wirth, R. (1992) Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells. Infect Immun 60, 25 30.
27. Leavis, H., Top, J., Shankar, N., Borgen, K., Bonten, M., van Embden, J. Willems, R.J. (2004) A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity. J Bacteriol 186, 672 682.
28. Lopes, M.F.S., Pereira, C.I., Rodrigues, F.M.S., Martins, M.P., Mimoso, M.C., Barros, T.C., Figueiredo Marques, J.J., Tenreiro, R.P. et al. (1999) Registered designation of origin areas of fermented food products defined by microbial phenotypes and artificial neural networks. Appl Environ Microbiol 65, 4484 4489. (Pubitemid 129519963)
29. Lowe, A.M., Lambert, P.A. Smith, A.W. (1995) Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci. Infect Immun 63, 703 706.
30. Mannu, L., Paba, A., Pes, M., Floris, R., Scintu, M.F. Morelli, L. (1999) Strain typing among enterococci isolated from homemade Pecorino Sardo cheese. FEMS Microbiol Lett 170, 25 30. (Pubitemid 29044070)
31. Massol-Deya, A., Odelson, D.A., Hickey, R.F. Tiedje, J.M. (1995) Bacterial community fingerprinting of amplified 16S and 16-23S ribosomal DNA gene sequences and restriction endonuclease analysis (ARDRA). In Molecular Microbial Ecology Manual ed. Akkermans, A.D.L., van Elsas, J.D. de Bruijn, F.J. pp. 3.3.2 : 1 8.
32. Moellering, R.C. Jr. Krogstad, D.J. (1979) Antibiotic resistance in enterococci. In Microbiology ed. Schlessinger, D. pp. 293 298.
33. Pitcher, D.G., Saunders, N.A. Owen, R.J. (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151 156.
34. Sartingen, S., Rozdzinski, E., Muscholl-Silberhorn, A. Marre, R. (2000) Aggregation substance increases adherence and internalization, but not translocation, of Enterococcus faecalis through different intestinal epithelial cells in vitro. Infect Immun 68, 6044 6047.
35. Schlievert, P.M., Gahr, P.J., Assimacopoulos, A.P., Dinges, M.M., Stoehr, J.A., Harmala, J.W., Hirt, H. Dunny, G.M. (1998) Aggregation and binding substances enhance pathogenicity in rabbit models of Enterococcus faecalis endocarditis. Infect Immun 66, 218 223. (Pubitemid 28018823)
36. Semedo, T., Santos, M.A., Lopes, M.F., Figueiredo-Marques, J.J., Barreto-Crespo, M.T. Tenreiro, R. (2003a) Virulence factors in food, clinical and reference enterococci: a common trait in the genus? Syst Appl Microbiol 26, 13 22. (Pubitemid 36514239)
37. Semedo, T., Santos, M.A., Martins, P., Lopes, M.F., Figueiredo-Marques, J.J., Tenreiro, R. Barreto-Crespo, M.T. (2003b) Comparative study using type strains and clinical and food isolates to examine hemolytic activity and occurrence of cyl operon in enterococci. J Clin Microbiol 41, 2569 2576. (Pubitemid 36712469)
38. Seno, Y., Kariyama, R., Mitsuhata, R., Monden, K. Kumon, H. (2005) Clinical implications of biofilm formation by Enterococcus faecalis in the urinary tract. Acta Med Okayama 59, 79 87. (Pubitemid 40935150)
39. Shaked, H., Carmeli, Y., Schwartz, D. Siegman-Igra, Y. (2006) Enterococcal bacteraemia: epidemiological, microbiological, clinical and prognostic characteristics, and the impact of high level gentamicin resistance. Scand J Infect Dis 38, 995 1000. (Pubitemid 44869270)
40. Shankar, V., Baghdayan, A.S., Huycke, M.M., Lindahl, G. Gilmore, M.S. (1999) Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect Immun 67, 193 200. (Pubitemid 29021648)
41. Shankar, V., Lockatell, C.V., Bagdhayan, A.S., Dranchenberg, C., Gilmore, M.S. Johnson, D.E. (2001) Role of Enterococcus faecalis surface protein esp in the pathogenesis of ascending urinary tract infection. Infect Immun 69, 4366 4372.
42. Shankar, N., Baghdayan, A.S. Gilmore, M.S. (2002) Modulation of virulence within a pathogenicity island in vancomycin-resistant Enterococcus faecalis. Nature 417, 746 750. (Pubitemid 34640628)
43. Shankar, N., Coburn, P., Pillar, C., Haas, W. Gilmore, M. (2004) Enterococcal cytolysin: activities and association with other virulence traits in a pathogenicity island. Int J Med Microbiol 293, 1 10. (Pubitemid 38667586)
44. Shepard, B.D. Gilmore, M.S. (2002) Differential expression of virulence-related genes in Enterococcus faecalis in response to biological cues in serum and urine. Infect Immun 70, 4344 4352.
45. Singh, K.V., Coque, T.M., Weinstock, G.M. Murray, B.E. (1998) In vivo testing of an Enterococcus faecalis efaA mutant and use of efaA homologs for species identification. FEMS Immunol Med Microbiol 21, 323 331.
46. Stevens, M.P. Edmond, M.B. (2005) Endocarditis due to vancomycin-resistant enterococci: case report and review of the literature. Clin Infect Dis 41, 1134 1142. (Pubitemid 41429476)
47. Tomich, P.K., An, F.Y., Damle, S.P. Clewell, D.B. (1979) Plasmid-related transmissibility and multiple drug resistance in Streptococcus faecalis subsp. zymogenes strain DS16. Antimicrob Agents Chemother 15, 828 830.
48. Tsakalidou, E., Manolopoulou, E., Tsilibari, V., Georgalaki, M. Kalantzopoulos, G. (1993) Esterolytic activities of Enterococcus durans and Enterococcus faecium strains isolated from Greek cheese. Neth Milk Dairy J 47, 145 150. (Pubitemid 2033529)
49. Wade, J.J. Uttley, A.H.C. (1996) Resistant enterococci mechanisms, laboratory detection and control in hospitals. J Clin Pathol 49, 700 703.
50. Willems, R.J.L., Homan, W., Top, J., van Santen-Verheuvel, M., Tribe, D., Manzioros, X., Gaillard, C., Vandenbroucke-Grauls, C.M.J.E. et al. (2001) Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. Lancet 357, 853 855.
51. Wirth, R. (1994) The sex pheromone system of Enterococcus faecalis. More than just a plasmid-collection mechanism? Eur J Biochem 222, 235 246.
52. Wirth, R. (2000) Sex pheromones and gene transfer in Enterococcus faecalis. Res Microbiol 151, 493 496.