In vivo testing of an Enterococcus faecalis efaA mutant and use of efaA homologs for species identification

FEMS Immunology and Medical Microbiology

Volumn 21, Issue 4, 1998, Pages 323-331

  Singh, Kavindra V ^a   Coque, Teresa M ^a   Weinstock, George M ^a   Murray, Barbara E ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

efaA mutant; Enterococcus; Species identification; Virulence

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM DETECTION; BACTERIUM MUTANT; ENTEROCOCCUS FAECALIS; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; SPECIES DIFFERENCE;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BASE SEQUENCE; DNA GYRASE; DNA PROBES; DNA TOPOISOMERASES, TYPE II; DNA, BACTERIAL; ENTEROCOCCUS FAECALIS; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; MUTATION;

EID: 0031697165     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(98)00087-X     Document Type: Article

References (33)

1. Lowe, A.M., Lambert, P.A. and Smith, A.W. (1995) Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci. Infect. Immun. 63, 703-706.
2. Jenkinson, H.F. (1994) Cell surface protein receptors in oral streptococci. FEMS Microbiol. Lett. 121, 133-140.
3. Berry, A.M. and Paton, J.C. (1996) Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae. Infect. Immun. 64, 5255-5262.
4. Correia, F.F., DiRienzo, J.M., McKay, T.L. and Rosan, B. (1996) scbA from Streptococcus crista CC5A: an atypical member of the lraI gene family. Infect. Immun. 64, 2114-2121.
5. Viscount, H.B., Munro, C.L., Burnette-Curley, D., Peterson, D.L. and Macrina, F.L. (1997) Immunization with FimA protects against Streptococcus parasanguis, endocarditis in rats. Infect. Immun. 65, 994-1002.
6. 2+ uptake. J Bacteriol. 180, 290-295.
7. Anderson, R.N., Ganeshkumar, N. and Kolenbrander, P.E. (1993) Cloning of the Streptococcus gordonii PK488 gene, encoding an adhesin which mediates coaggregation with Actinomyces naeslundii PK606. Infect. Immun. 61, 981-987.
8. Xu, Y., Jiang, L.X., Murray, B.E. and Weinstock, G.M. (1997) Enterococcus faecalis antigens in human infections. Infect. Immun. 65, 4207-4215.
9. Dybig, K., Hollingshead, S.K., Heath, D.G., Clewell, D.B., Sun, F. and Woodard, A. (1992) Degenerate oligonucleotide primers for enzymatic amplification of recA sequences from gram-positive bacteria and mycoplasmas. J. Bacteriol. 174, 2729-2732.
10. Korten, V., Huang, W.M. and Murray, B.E. (1994) Analysis by PCR and direct DNA sequencing of gyrA mutations associated with fluoroquinolone resistance in Enterococcus faecalis. Antimicrob. Agents Chemother. 38, 2091-2094.
11. Teixeira, L.M., Facklam, R.R., Steigerwalt, A.G., Pigott, N.E., Merquior, V.L.C. and Brenner, D.J. (1995) Correlation between phenotypic characteristics and DNA relatedness within Enterococcus faecium strains. J. Clin. Microbiol. 33, 1520-1523.
12. Facklam, R.R. and Sahm, D.A. (1995) Enterococcus. In: Manual of Clinical Microbiology (Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. and Yolken, R.H., Eds.), pp. 308-314. American Society of Microbiology, Washington, DC.
13. Coque, T.M., Tomayko, J.F., Ricke, S.C., Okhuysen, P.C. and Murray, B.E. (1996) Vancomycin-resistant enterococci from nosocomial, community, and animal sources in the United States. Antimicrob. Agents Chemother. 40, 2605-2609.
14. Singh, K.V., Qin, X., Weinstock, G.M. and Murray, B.E. (1998) Generation and testing of mutants of Enterococcus faecalis in a mouse peritonitis model. J. Infect. Dis. (in press).
15. Murray, B.E., Singh, K.V., Ross, R.P., Heath, J.D., Dunny, G.M. and Weinstock, G.M. (1993) Generation of restriction map of Enterococcus faecalis strain OG1 and investigation of growth requirements and regions encoding biosynthetic function. J. Bacteriol. 175, 5216-5223.
16. Friesenegger, A., Fiedler, S., Devriese, L.A. and Wirth, R. (1991) Genetic transformation of various species of Enterococcus by electroporation. FEMS Microbiol. Lett. 79, 323-328.
17. Li, X., Weinstock, G.M. and Murray, B.E. (1995) Generation of auxotrophic mutants of Enterococcus faecalis. J. Bacteriol. 177, 6866-6873.
18. Harlow, E. and Lane, D. (1988) Immunoblotting. In: Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Chenoweth, C.E., Robinson, K.A. and Schaberg, D.R. (1990) Efficacy of ampicillin versus trimethoprim-sulfamethoxazole in a mouse model of lethal enterococcal peritonitis. Antimicrob. Agents Chemother. 34, 1800-1802.
20. Gollapudi, S.V.S., Gupta, A., Thadepalli, H. and Perez, A. (1988) Use of lymphokines in treatment of experimental intraabdominal abscess caused by Bacteroides fragilis. Infect. Immun. 56, 2369-2372.
21. Reed, L.J. and Muench, H. (1938) A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27, 493-497.
22. Coque, T.M., Patterson, J.E., Steckelberg, J.M. and Murray, B.E. (1995) Incidence of hemolysin, gelatinase and aggregative substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. J. Infect. Dis. 171, 1223-1229.
23. Shizuya, H., Birren, B., Kim, U.-J., Mancino, V., Slepak, T., Tachiiri, Y. and Simon, M. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797.
24. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Su, Y.A., Sulavik, M.C., He, P., Makinen, K.K., Makinen, P.L., Fielder, S., Wirth, R. and Clewell, D.B. (1991) Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infect. Immun. 59, 415-420.
26. Burnette-Curley, D., Wells, V., Viscount, H., Munro, C.L., Fenno, J.C., Fives-Taylor, P. and Macrina, F.L. (1995) FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis. Infect. Immun. 63, 4669-4674.
27. Kolenbrander, P.E., Andersen, R.N. and Ganeshkumar, N. (1994) Nucleotide sequence of the Streptococcus gordonii PK488 coaggregation adhesin gene and ATP-binding cassette. Infect. Immun. 62, 4469-4480.
28. Fenno, J.C., LeBlanc, D.J. and Fives-Taylor, P. (1989) Nucleotide sequence analysis of a type 1 fimbrial gene of Streptococcus sanguis FW213. Infect. Immun. 57, 3527-3533.
29. Sampson, J.S., O'Conner, S.P., Stinson, A.R., Tharpe, J.A. and Russel, H. (1994) Cloning and nucleotide sequence analysis of psaA, the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect. Immun. 62, 319-324.
30. Merquior, V.L.C., Peralta, J.M., Facklam, R.R. and Teixeira, L. M. (1994) Analysis of electrophoretic whole-cell protein profiles as a tool for characterization of Enterococcus species. Curr. Microbiol. 28, 149-153.
31. Daly, J.A., Clifton, N.L., Seskin, K.C. and Manford Gooch, W. (1991) Use of rapid, nonradioactive DNA probes in culture confirmation tests to detect Streptococcus agalactiae, Haemophilus influenzae, and Enterococcus spp. from pediatric patients with significant infections. J. Clin. Microbiol. 29, 80-82.
32. Davis, T.E. and Fuller, D.D. (1991) Direct identification of bacterial isolates in blood cultures by using a DNA probe. J. Clin. Microbiol. 29, 2193-2196.
33. Coque, T.M. and Murray, B.E. (1996) Identification of Enterococcus faecalis strains by DNA hybridization and pulsed-field gel electrophoresis. J. Clin. Microbiol. 33, 3368-3369.