Assessment of a spectrophotometric assay for monoacylglycerol lipase activity

AAPS Journal

Volumn 12, Issue 2, 2010, Pages 197-201

  Ulloa, Nadine M ^a   Deutsch, Dale G ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Monoacylglycerol lipase; Spectrophotometric assay

Indexed keywords

ACYLGLYCEROL LIPASE;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CHEMICAL PARAMETERS; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; EXPERIMENTAL STUDY; GENE MUTATION; MOUSE; NONHUMAN; RADIOMETRY; SENSITIVITY ANALYSIS; SPECTROPHOTOMETRY; STRUCTURE ANALYSIS;

ANIMALS; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; COS CELLS; CYTOSOL; ENZYME INHIBITORS; GLYCERIDES; KINETICS; MONOACYLGLYCEROL LIPASES; MUTAGENESIS; RADIOMETRY; TRANSFECTION;

EID: 77950636132     PISSN: 15507416     EISSN: None     Source Type: Journal    
DOI: 10.1208/s12248-010-9180-6     Document Type: Article

References (20)

1. Gaoni Y, Mechoulam R. Isolation, structure and partial synthesis of an active constituent of hashish. J Am Chem Soc. 1964;86: 1646-1647
2. Devane WA, Dysarz 3rd FA, Johnson MR, Melvin LS, Howlett AC. Determination and characterization of a cannabinoid receptor in rat brain. Mol Pharmacol. 1988;34(5):605-613 (Pubitemid 19007296)
3. Munro S, Thomas KL, Abu-Shaar M. Molecular characterization of a peripheral receptor for cannabinoids. Nature. 1993;365 (6441):61-65 (Pubitemid 23305569)
4. Sugiura T, Kishimoto S, Oka S, Gokoh M. Biochemistry, pharmacology and physiology of 2-arachidonoylglycerol, an endogenous cannabinoid receptor ligand. Prog Lipid Res. 2006;45(5):405-446 (Pubitemid 43912907)
5. Mechoulam R, Ben-Shabat S, Hanus L, Ligumsky M, Kaminski NE, Schatz AR, et al. Identification of an endogenous 2-monoglyceride, present in canine gut, that binds to cannabinoid receptors. Biochem Pharmacol. 1995;50(1):83-90.
6. Dinh TP, Carpenter D, Leslie FM, Freund TF, Katona I, Sensi SL, et al. Brain monoglyceride lipase participating in endocannabinoid inactivation. Proc Natl Acad Sci USA. 2002;99 (16):10819-10824
7. Gulyas AI, Cravatt BF, Bracey MH, Dinh TP, Piomelli D, Boscia F, et al. Segregation of two endocannabinoid-hydrolyzing enzymes into pre-and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala. Eur J Neurosci. 2004;20 (2):441-458 (Pubitemid 38969629)
8. Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol. 2007;14(12):1347-1356
9. Karlsson M, Contreras JA, Hellman U, Tornqvist H, Holm C. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. J Biol Chem. 1997;272(43):27218-27223 (Pubitemid 27452682)
10. Chau LY, Tai HH. Monoglyceride and diglyceride lipases from human platelet microsomes. Biochim Biophys Acta. 1988;963 (3):436-444
11. Saario SM, Laitinen JT. Monoglyceride lipase as an enzyme hydrolyzing 2- arachidonoylglycerol. Chem Biodivers. 2007;4 (8):1903-1913
12. Riddles PW, Blakeley RL, Zerner B. Reassessment of Ellman's reagent. Methods Enzymol. 1983;91:49-60.
13. King AR, Lodola A, Carmi C, Fu J, Mor M, Piomelli D. A critical cysteine residue in monoacylglycerol lipase is targeted by a new class of isothiazolinone-based enzyme inhibitors. Br J Pharmacol. 2009;157(6):974-983
14. Saario SM, Salo OM, Nevalainen T, Poso A, Laitinen JT, Jarvinen T, et al. Characterization of the sulfhydryl-sensitive site in the enzyme responsible for hydrolysis of 2-arachidonoylglycerol in rat cerebellar membranes. Chem Biol. 2005;12 (6):649-656 (Pubitemid 43142042)
15. Zvonok N, Pandarinathan L,Williams J, Johnston M, Karageorgos I, Janero DR, et al. Covalent inhibitors of human monoacylglycerol lipase: ligand-assisted characterization of the catalytic site by mass spectrometry and mutational analysis. Chem Biol. 2008;15(8):854-862
16. Heckman KL, Pease LR. Gene splicing and mutagenesis by PCR-driven overlap extension. Nat Protoc. 2007;2(4):924-932
17. Saario SM. Enzymatic hydrolysis of the endocannabinoid 2-arachidonoylglycerol-characterization and inhibition in rat brain membranes and homogenates. Kuopio: University of Kuopio; 2006.
18. Muccioli GG, Xu C, Odah E, Cudaback E, Cisneros JA, Lambert DM, et al. Identification of a novel endocannabinoidhydrolyzing enzyme expressed by microglial cells. J Neurosci. 2007;27(11):2883-2889 (Pubitemid 46438968)
19. Muccioli GG, Labar G, Lambert DM. CAY10499, a novel monoglyceride lipase inhibitor evidenced by an expeditious MGL assay. Chembiochem. 2008;9(16):2704-2710
20. Wang Y, Chanda P, Jones PG, Kennedy JD. A fluorescencebased assay for monoacylglycerol lipase compatible with inhibitor screening. Assay Drug Dev Technol. 2008;6(3):387-393