Cloning, overexpression, and characterization of recombinant heparinase III from Bacteroides stercoris HJ-15

Applied Microbiology and Biotechnology

Volumn 86, Issue 3, 2010, Pages 879-890

  Hyun, Yang Jin ^a   Lee, Jeong Hoon ^a   Kim, Dong Hyun ^a  

^a Kyung Hee University   (South Korea)

Author keywords

Bacteroides stercoris HJ 15; Characterization; Cloning and overexpression; Recombinant heparinase III; SAX HPLC

Indexed keywords

AFFINITY COLUMN; BACTEROIDES; BACTEROIDES STERCORIS HJ-15; DEGRADATION PRODUCTS; ENZYMATIC ACTIVITIES; ENZYMATIC PROPERTIES; GENBANK; GLYCOSAMINOGLYCANS; HEPARAN SULFATES; HYALURONIC ACIDS; KINETIC CONSTANT; ONE STEP; OVER-EXPRESSION; SITE DIRECTED MUTAGENESIS; SODIUM PHOSPHATE BUFFER; SOUTHERN BLOTTING; STRONG ANIONS; SUBSTRATE SPECIFICITY;

AMINO ACIDS; CLONING; DEGRADATION; ENZYMES; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC ACIDS; PURIFICATION; SODIUM; SODIUM CHLORIDE;

COLUMN CHROMATOGRAPHY;

GLYCOSAMINOGLYCAN; HEPARAN SULFATE; HEPARIN; HEPARIN LYASE; HYALURONIC ACID; LYASE; SODIUM DIHYDROGEN PHOSPHATE;

AMINO ACID; BACTERIUM; BIOASSAY; BIODEGRADATION; ENZYME ACTIVITY; GENE EXPRESSION; ION EXCHANGE; LIQUID CHROMATOGRAPHY; PHOSPHATE; POLYSACCHARIDE; REACTION KINETICS; SUBSTRATE;

ARTICLE; BACTEROIDES; BACTEROIDES STERCORIS; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE IDENTIFICATION; GENE SEQUENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; SOUTHERN BLOTTING;

AMINO ACID SUBSTITUTION; BACTEROIDES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; HEPARIN; HEPARITIN SULFATE; HYALURONIC ACID; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYSACCHARIDE-LYASES; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

BACTEROIDES STERCORIS; ESCHERICHIA COLI;

EID: 77950626844     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2327-7     Document Type: Article

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