Direct quantitative determination of ceramide glycosylation in vivo: A new approach to evaluate cellular enzyme activity of glucosylceramide synthase

Journal of Lipid Research

Volumn 51, Issue 4, 2010, Pages 866-874

  Gupta, Vineet ^a   Patwardhan, Gauri A ^a   Zhang, Qian Jin ^b   Cabot, Myles C ^c   Jazwinski, S Michal ^d   Liu, Yong Yu ^a  

^a UNIVERSITY OF LOUISIANA AT MONROE   (United States)

^b LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^c JOHN WAYNE CANCER INSTITUTE   (United States)

^d TULANE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cells; Ceramide; Enzyme; Glucosylceramide; Thin layer chromatograph; Tumor

Indexed keywords

CERAMIDE GLUCOSYLTRANSFERASE; SPHINGOSINE DERIVATIVE; URIDINE DIPHOSPHATE GLUCOSE; 2 DECANOYLAMINO 3 MORPHOLINO 1 PHENYL 1 PROPANOL; 4 CHLORO 7 NITROBENZOFURAZAN; CERAMIDE; ENZYME INHIBITOR; GLUCOSYLTRANSFERASE; MORPHOLINE DERIVATIVE; N-(7-(4-NITROBENZO-2-OXA-1,3-DIAZOLE))-6-AMINOCAPROYL SPHINGOSINE; OLIGONUCLEOTIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IN VIVO CULTURE; MALIGNANT NEOPLASTIC DISEASE; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SPECTROPHOTOMETRY; THIN LAYER CHROMATOGRAPHY; ANALOGS AND DERIVATIVES; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL MEMBRANE PERMEABILITY; COLONIC NEOPLASMS; COMPARATIVE STUDY; DRUG SCREENING; ENZYME ASSAY; FEMALE; GENETICS; GLYCOSYLATION; INTRALESIONAL DRUG ADMINISTRATION; METABOLISM; NUDE MOUSE; OVARIAN NEOPLASMS; PATHOLOGY; PROCEDURES; RANDOMIZATION; TUMOR CELL LINE; VALIDATION STUDY;

4-CHLORO-7-NITROBENZOFURAZAN; ANIMALS; CELL LINE, TUMOR; CELL MEMBRANE PERMEABILITY; CERAMIDES; CHROMATOGRAPHY, THIN LAYER; COLONIC NEOPLASMS; ENZYME ASSAYS; ENZYME INHIBITORS; FEMALE; GLUCOSYLTRANSFERASES; GLYCOSYLATION; HUMANS; INJECTIONS, INTRALESIONAL; MICE; MICE, NUDE; MORPHOLINES; OLIGONUCLEOTIDES; OVARIAN NEOPLASMS; RANDOM ALLOCATION; REPRODUCIBILITY OF RESULTS; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 77950600427     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D002949     Document Type: Article

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