A sensitive and reproducible assay to measure the activity of glucosylceramide synthase and lactosylceramide synthase using HPLC and fluorescent substrates

Analytical Biochemistry

Volumn 345, Issue 2, 2005, Pages 181-186

  Hayashi, Yasuhiro ^a   Horibata, Yasuhiro ^a   Sakaguchi, Keishi ^a   Okino, Nozomu ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Antisense morpholino oligo; Fluorescent substrate; Gene knockdown; Glucosylceramide synthase; Glycosphingolipid; High performance liquid chromatography; Lactosylceramide synthase; Zebrafish

Indexed keywords

GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REACTION PRODUCTS; SUBSTRATES;

ANTISENSE; GENE KNOCK DOWN; GLUCOSYLCERAMIDE SYNTHASE; GLYCOSPHINGOLIPIDS; SYNTHASES; ZEBRAFISH;

FLUORESCENCE;

4 NITROBENZ 2 OXA 1,3 DIAZOLE CERAMIDE; 4 NITROBENZ 2 OXA 1,3 DIAZOLE GLUCOSYLCERAMIDE; 4 NITROBENZ 2 OXA 1,3 DIAZOLE LACTOSYLCERAMIDE; CERAMIDE DERIVATIVE; CERAMIDE GLUCOSYLTRANSFERASE; ISOENZYME; LACTOSYLCERAMIDE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LYSATE; CELL STRAIN; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EMBRYO; EMBRYO CELL; EMBRYO DEVELOPMENT; ENZYME ACTIVITY; ENZYME ASSAY; FEMALE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; TIME; ZEBRA FISH;

CRICETULUS GRISEUS; DANIO RERIO; VERTEBRATA;

EID: 25444458618     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.05.029     Document Type: Article

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