The Mycobacterium tuberculosis protein Ldt Mt2 is a nonclassical transpeptidase required for virulence and resistance to amoxicillin

Nature Medicine

Volumn 16, Issue 4, 2010, Pages 466-469

  Gupta, Radhika ^a   Lavollay, Marie ^b,c,d   Mainardi, Jean Luc ^b,c,d,e   Arthur, Michel ^b,c,d   Bishai, William R ^a   Lamichhane, Gyanu ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CENTRE DE RECHERCHE DES CORDELIERS   (France)

^c UNIVERSITÉ PARIS DESCARTES   (France)

^d INSERM   (France)

^e HÔPITAL EUROPÉEN GEORGES POMPIDOU   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMOXICILLIN; AMOXICILLIN PLUS CLAVULANIC ACID; BACTERIAL PROTEIN; BETA LACTAM; GAMMA GLUTAMYLTRANSFERASE; MEROPENEM; PROTEIN LDT MT2; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; CONTROLLED STUDY; FEMALE; MOUSE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION;

AMINOACYLTRANSFERASES; AMOXICILLIN; ANIMALS; ANTITUBERCULAR AGENTS; HUMANS; MICE; MICE, INBRED BALB C; MICROBIAL SENSITIVITY TESTS; MODELS, BIOLOGICAL; MYCOBACTERIUM TUBERCULOSIS; PENICILLIN RESISTANCE; PEPTIDOGLYCAN; TUBERCULOSIS, PULMONARY;

BACILLI (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS;

EID: 77950534682     PISSN: 10788956     EISSN: 1546170X     Source Type: Journal    
DOI: 10.1038/nm.2120     Document Type: Article

References (29)

1. Wietzerbin, J. et al. Occurrence of D-alanyl-(D)-meso-diaminopimelic acid and meso-diaminopimelyl-meso-diaminopimelic acid interpeptide linkages in the peptidoglycan of Mycobacteria. Biochemistry 13, 3471-3476 (1974).
2. Lavollay, M. et al. The peptidoglycan of stationary-phase Mycobacterium tuberculosis predominantly contains cross-links generated by L,D-transpeptidation. J. Bacteriol. 190, 4360-4366 (2008). (Pubitemid 351812884)
3. Hugonnet, J.E., Tremblay, L.W., Boshoff, H.I., Barry, C.E. III & Blanchard, J.S. Meropenem-clavulanate is effective against extensively drug-resistant Mycobacterium tuberculosis. Science 323, 1215-1218 (2009).
4. Fauci, A.S. Multidrug-resistant and extensively drug-resistant tuberculosis: the National Institute of Allergy and Infectious Diseases Research agenda and recommendations for priority research. J. Infect. Dis. 197, 1493-1498 (2008).
5. Gandhi, N.R. et al. Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet 368, 1575-1580 (2006).
6. Jindani, A., Dore, C.J. & Mitchison, D.A. Bactericidal and sterilizing activities of antituberculosis drugs during the frst 14 days. Am. J. Respir. Crit. Care Med. 167, 1348-1354 (2003). (Pubitemid 36609718)
7. Wayne, L.G. & Sohaskey, C.D. Nonreplicating persistence of Mycobacterium tuberculosis. Annu. Rev. Microbiol. 55, 139-163 (2001).
8. Betts, J.C., Lukey, P.T., Robb, L.C., McAdam, R.A. & Duncan, K. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profling. Mol. Microbiol. 43, 717-731 (2002). (Pubitemid 34597265)
9. Voskuil, M.I. et al. Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program. J. Exp. Med. 198, 705-713 (2003).
10. Keren, I., Shah, D., Spoering, A., Kaldalu, N. & Lewis, K. Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli. J. Bacteriol. 186, 8172-8180 (2004). (Pubitemid 39603206)
11. Goren, M.B. & Brennan, P.J. Tuberculosis (ed. Youmans, G.P.) 63 (W. B. Saunders, Philadelphia, 1979).
12. Vollmer, W. & Holtje, J.V. The architecture of the murein (peptidoglycan) in Gram-negative bacteria: vertical scaffold or horizontal layer(s)? J. Bacteriol. 186, 5978-5987 (2004). (Pubitemid 39186985)
13. Matsuhashi, M. [Biosynthesis in the bacterial cell wall] Tanpakushitsu Kakusan Koso 11, 875-886 (1966).
14. Lamichhane, G. et al. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 100, 7213-7218 (2003). (Pubitemid 36706418)
15. Mainardi, J.L. et al. A novel peptidoglycan cross-linking enzyme for a β-lactam-resistant transpeptidation pathway. J. Biol. Chem. 280, 38146-38152 (2005).
16. Lavollay, M. et al. The β-lactam-sensitive D,D-carboxypeptidase activity of Pbp4 controls the L,D and D,D transpeptidation pathways in Corynebacterium jeikeium. Mol. Microbiol. (in the press) (2009).
17. Hugonnet, J.E. & Blanchard, J.S. Irreversible inhibition of the Mycobacterium tuberculosis β-lactamase by clavulanate. Biochemistry 46, 11998-12004 (2007). (Pubitemid 350022357)
18. Donald, P.R. et al. Early bactericidal activity of amoxicillin in combination with clavulanic acid in patients with sputum smear-positive pulmonary tuberculosis. Scand. J. Infect. Dis. 33, 466-469 (2001).
19. Nadler, J.P., Berger, J., Nord, J.A., Cofsky, R. & Saxena, M. Amoxicillin-clavulanic acid for treating drug-resistant Mycobacterium tuberculosis. Chest 99, 1025-1026 (1991).
20. Ghuysen, J.M. Serine β-lactamases and penicillin-binding proteins. Annu. Rev. Microbiol. 45, 37-67 (1991).
21. Waxman, D.J. & Strominger, J.L. Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics. Annu. Rev. Biochem. 52, 825-869 (1983). (Pubitemid 13030694)
22. Crick, D.C. & Brennan, P.J. Biosynthesis of the arabinogalactan- peptidoglycan complex. in The Mycobacterial Cell Envelope (eds. Daffe, M. & Reyrat, J.) 25-40 (American Society for Microbiology, Washington, DC, 2008).
23. Templin, M.F., Ursinus, A. & Holtje, J.V. A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli. EMBO J. 18, 4108-4117 (1999). (Pubitemid 29364114)
24. Boneca, I.G. et al. A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system. Proc. Natl. Acad. Sci. USA 104, 997-1002 (2007).
25. Lee, M.H., Pascopella, L., Jacobs, W.R. Jr. & Hatfull, G.F. Site-specifc integration of mycobacteriophage L5: integration-profcient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. USA 88, 3111-3115 (1991). (Pubitemid 21916569)
26. Amrein, K.E. et al. Purifcation and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL. Proc. Natl. Acad. Sci. USA 92, 1048-1052 (1995).
27. Auger, G., van Heijenoort, J., Mengin-Lecreulx, D. & Blanot, D.A. MurG assay which utilises a synthetic analogue of lipid I. FEMS Microbiol. Lett. 219, 115-119 (2003). (Pubitemid 36194787)
28. Arbeloa, A. et al. Synthesis of mosaic peptidoglycan cross-bridges by hybrid peptidoglycan assembly pathways in Gram-positive bacteria. J. Biol. Chem. 279, 41546-41556 (2004). (Pubitemid 39313596)
29. Wiegand, I., Hilpert, K. & Hancock, R.E. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3, 163-175 (2008). (Pubitemid 351266501)