Direct evidence from single-cell analysis that human α-defensins block adenovirus uncoating to neutralize infection

Journal of Virology

Volumn 84, Issue 8, 2010, Pages 4041-4049

  Nguyen, Emily K ^a   Nemerow, Glen R ^a   Smithf, Jason G ^a,b  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA DEFENSIN; BROXURIDINE; COAT PROTEIN; ALPHA DEFENSIN 5, HUMAN; ALPHA-DEFENSIN 5, HUMAN; VIRUS PROTEIN;

ADENOVIRUS; ADENOVIRUS INFECTION; ANTIVIRAL ACTIVITY; ARTICLE; ASSAY; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNE RESPONSE; MICROBIAL KINETICS; PRIORITY JOURNAL; VIRUS GENOME; VIRUS PARTICLE; CELL LINE; CONFOCAL MICROSCOPY; HUMAN ADENOVIRUS; IMMUNOLOGY; METABOLISM; METHODOLOGY; PHYSIOLOGY; STAINING; VIRUS CAPSID; VIRUS ENTRY;

ADENOVIRIDAE; HUMAN ADENOVIRUS; MIRIDAE;

ADENOVIRIDAE INFECTIONS; ADENOVIRUSES, HUMAN; ALPHA-DEFENSINS; BROMODEOXYURIDINE; CAPSID; CELL LINE; HUMANS; MICROSCOPY, CONFOCAL; STAINING AND LABELING; VIRAL PROTEINS; VIRUS INTERNALIZATION;

EID: 77950470139     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.02471-09     Document Type: Article

References (34)

1. Arnberg, N. 2009. Adenovirus receptors: implications for tropism, treatment and targeting. Rev. Med. Virol. 19: 165-178.
2. Bailey, C. J., R. G. Crystal, and P. L. Leopold. 2003. Association of adenovirus with the microtubule organizing center. J. Virol. 77: 13275-13287.
3. Berns, K. I., and S. Adler. 1972. Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains. J. Virol. 9: 394-396.
4. Berns, K. I., and J. A. Rose. 1970. Evidence for a single-stranded adenovirusassociated virus genome: isolation and separation of complementary single strands. J. Virol. 5: 693-699.
5. Cerullo, V., M. P. Seiler, V. Mane, N. Brunetti-Pierri, C. Clarke, T. K. Bertin, J. R. Rodgers, and B. Lee. 2007. Toll-like receptor 9 triggers an innate immune response to helper-dependent adenoviral vectors. Mol. Ther. 15: 378-385.
6. Chardonnet, Y., and S. Dales. 1970. Early events in the interaction of adenoviruses with HeLa cells. I. Penetration of type 5 and intracellular release of the DNA genome. Virology 40: 462-477.
7. Dugan, A. S., M. S. Maginnis, J. A. Jordan, M. L. Gasparovic, K. Manley, R. Page, G. Williams, E. Porter, B. A. O'Hara, and W. J. Atwood. 2008. Human alpha-defensins inhibit BK virus infection by aggregating virions and blocking binding to host cells. J. Biol. Chem. 283: 31125-31132.
8. Evans, R. K., D. K. Nawrocki, L. A. Isopi, D. M. Williams, D. R. Casimiro, S. Chin, M. Chen, D. M. Zhu, J. W. Shiver, and D. B. Volkin. 2004. Development of stable liquid formulations for adenovirus-based vaccines. J. Pharm. Sci. 93: 2458-2475.
9. Fejer, G., L. Drechsel, J. Liese, U. Schleicher, Z. Ruzsics, N. Imelli, U. F. Greber, S. Keck, B. Hildenbrand, A. Krug, C. Bogdan, and M. A. Freudenberg. 2008. Key role of splenic myeloid DCs in the IFN-alphabeta response to adenoviruses in vivo. PLoS Pathog. 4: e1000208.
10. Greber, U. F., M. Willetts, P. Webster, and A. Helenius. 1993. Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75: 477-486.
11. Iacobelli-Martinez, M., and G. R. Nemerow. 2007. Preferential activation of Toll-like receptor nine by CD46-utilizing adenoviruses. J. Virol. 81: 1305-1312.
12. Imelli, N., Z. Ruzsics, D. Puntener, M. Gastaldelli, and U. F. Greber. 2009. Genetic reconstitution of the human adenovirus type 2 temperature- sensitive 1 mutant defective in endosomal escape. Virol. J. 6: 174.
13. Kjellen, L., H. G. Pereira, R. C. Valentine, and J. A. Armstrong. 1963. An analysis of adenovirus particles and soluble antigens produced in the presence of 5-bromodeoxyuridine. Nature 199: 1210-1211.
14. Lawrence, W. C., and H. S. Ginsberg. 1967. Intracellular uncoating oftype 5 adenovirus deoxyribonucleic acid. J. Virol. 1: 851-867.
15. Leopold, P. L., and R. G. Crystal. 2007. Intracellular trafficking of adenovirus: many means to many ends. Adv. Drug Deliv. Rev. 59: 810-821.
16. Lonberg-Holm, K., and L. Philipson. 1969. Early events of virus-cell interaction in an adenovirus system. J. Virol. 4: 323-338.
17. Mirza, M. A., and J. Weber. 1979. Uncoating of adenovirus type 2. J. Virol. 30: 462-471.
18. Muruve, D. A., V. Petrilli, A. K. Zaiss, L. R. White, S. A. Clark, P. J. Ross, R. J. Parks, and J. Tschopp. 2008. The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response. Nature 452: 103-107.
19. Nakano, M. Y., K. Boucke, M. Suomalainen, R. P. Stidwill, and U. F. Greber. 2000. The first step of adenovirus type 2 disassembly occurs at the cell surface, independently of endocytosis and escape to the cytosol. J. Virol. 74: 7085-7095.
20. Nociari, M., O. Ocheretina, J. W. Schoggins, and E. Falck-Pedersen. 2007. Sensing infection by adenovirus: Toll-like receptor-independent viral DNA recognition signals activation of the interferon regulatory factor 3 master regulator. J. Virol. 81: 4145-4157.
21. Perez-Berna, A. J., R. Marabini, S. H. Scheres, R. Menendez-Conejero, I. P. Dmitriev, D. T. Curiel, W. F. Mangel, S. J. Flint, and C. San Martin. 2009. Structure and uncoating of immature adenovirus. J. Mol. Biol. 392: 547-557.
22. Prage, L., U. Pettersson, S. Hoglund, K. Lonberg-Holm, and L. Philipson. 1970. Structural proteins of adenoviruses. IV. Sequential degradation of the adenovirus type 2 virion. Virology 42: 341-358.
23. Rosenke, K., and E. A. Fortunato. 2004. Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection. J. Virol. 78: 7818-7822.
24. Russell, W. C., R. C. Valentine, and H. G. Pereira. 1967. The effect of heat on the anatomy of the adenovirus. J. Gen. Virol. 1: 509-522.
25. Silvestry, M., S. Lindert, J. G. Smith, O. Maier, C. M. Wiethoff, G. R. Nemerow, and P. L. Stewart. 2009. Cryo-electron microscopy structure of adenovirus type 2 temperature-sensitive mutant 1 reveals insight into the cell entry defect. J. Virol. 83: 7375-7383.
26. Smith, J. G., A. Cassany, L. Gerace, R. Ralston, and G. R. Nemerow. 2008. Neutralizing antibody blocks adenovirus infection by arresting microtubuledependent cytoplasmic transport. J. Virol. 82: 6492-6500.
27. Smith, J. G., and G. R. Nemerow. 2008. Mechanism of adenovirus neutralization by human alpha-defensins. Cell Host Microbe 3: 11-19.
28. Strunze, S., L. C. Trotman, K. Boucke, and U. F. Greber. 2005. Nuclear targeting of adenovirus type 2 requires CRM1-mediated nuclear export. Mol. Biol. Cell 16: 2999-3009.
29. Sussenbach, J. S. 1967. Early events in the infection process of adenovirus type 5 in HeLa cells. Virology 33: 567-574.
30. Svensson, U., and R. Persson. 1984. Entry of adenovirus 2 into HeLa cells. J. Virol. 51: 687-694.
31. Warming, S., N. Costantino, D. L. Court, N. A. Jenkins, and N. G. Copeland. 2005. Simple and highly efficient BAC recombineering using galK selection. Nucleic Acids Res. 33: e36.
32. Wiethoff, C. M., H. Wodrich, L. Gerace, and G. R. Nemerow. 2005. Adenovirus protein VI mediates membrane disruption following capsid disassembly. J. Virol. 79: 1992-2000.
33. Wohlfart, C. 1988. Neutralization of adenoviruses: kinetics, stoichiometry, and mechanisms. J. Virol. 62: 2321-2328.
34. Zhu, J., X. Huang, and Y. Yang. 2007. Innate immune response to adenoviral vectors is mediated by both Toll-like receptor-dependent and-independent pathways. J. Virol. 81: 3170-3180.