Relaxing the coenzyme specificity of 1,3-propanediol oxidoreductase from Klebsiella pneumoniae by rational design

Journal of Biotechnology

Volumn 146, Issue 4, 2010, Pages 173-178

  Ma, Chengwei ^a   Zhang, Le ^a   Dai, Jianying ^a   Xiu, Zhilong ^a  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

1,3 Propanediol oxidoreductase; Coenzyme specificity; Computational alanine scanning mutagenesis; Klebsiella pneumoniae; Rational protein design; Site directed mutagenesis

Indexed keywords

ALANINE-SCANNING MUTAGENESIS; COENZYME SPECIFICITY; KLEBSIELLA PNEUMONIAE; OXIDOREDUCTASES; PROPANEDIOLS; PROTEIN DESIGN; SITE DIRECTED MUTAGENESIS;

BINDING ENERGY; COMPETITION; DESIGN; GLYCEROL; PROTEINS; SCANNING;

MUTAGENESIS;

1,3 PROPANEDIOL OXIREDUCTASE; ADENINE; ALANINE; ASPARTIC ACID; GLYCINE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CELL ENERGY; COENZYME SPECIFICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SPECIFICITY; KLEBSIELLA PNEUMONIAE; MATHEMATICAL ANALYSIS; MOLECULAR CLONING; MOLECULAR DYNAMICS; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SIMULATION; SITE DIRECTED MUTAGENESIS;

ALANINE; ALCOHOL OXIDOREDUCTASES; ANAEROBIOSIS; BACTERIAL PROTEINS; COENZYMES; GLYCINE; HYDROGEN BONDING; KLEBSIELLA PNEUMONIAE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

KLEBSIELLA PNEUMONIAE;

EID: 77950461136     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.02.005     Document Type: Article

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