Alteration of coenzyme specificity of lactate dehydrogenase from Thermus thermophilus by introducing the loop region of NADP(H)-dependent malate dehydrogenase

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 9, 2006, Pages 2230-2235

  Tomita, Takeo ^a   Kuzuyama, Tomohisa ^a   Nishiyama, Makoto ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b RIKEN SPring 8 Center   (Japan)

Author keywords

Coenzyme specificity; Lactate dehydrogenase; Thermus thermophilus

Indexed keywords

COENZYME SPECIFICITY; LACTATE DEHYDROGENASE; MUTANT ENZYMES; THERMUS THERMOPHILUS;

AMINO ACIDS; BACTERIOLOGY; COENZYMES; MUTAGENESIS;

ENZYME KINETICS;

BACTERIAL DNA; LACTATE DEHYDROGENASE; MALATE DEHYDROGENASE (NADP); NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PLASMID; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; THERMUS THERMOPHILUS;

AMINO ACID SEQUENCE; BINDING SITES; COMPUTER SIMULATION; DNA, BACTERIAL; KINETICS; L-LACTATE DEHYDROGENASE; MALATE DEHYDROGENASE (NADP+); MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NAD; NADP; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; THERMUS THERMOPHILUS;

THERMUS THERMOPHILUS;

EID: 33749004786     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60170     Document Type: Article

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