Simple and effective gap-repair cloning using short tracts of flanking homology in fission yeast

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 3, 2010, Pages 685-689

  Matsuo, Yuzy ^a   Kishimoto, Hayafumi ^a   Horiuchi, Tomitaka ^a   Tanae, Katsuhiro ^a   Kawamukai, Makoto ^a  

^a SHIMANE UNIVERSITY   (Japan)

Author keywords

Fission yeast; Gap repair cloning; One step cloning of multiple DNA fragments; Short tracts of flanking homology

Indexed keywords

DNA FRAGMENT; FISSION YEAST; FUSION GENES; GAP-REPAIR CLONING; MARKER GENES; ONE-STEP CLONING;

DNA; GENES; MICROBIOLOGY; REPAIR; YEAST;

CLONING;

ARTICLE; DNA REPAIR; GENE EXPRESSION REGULATION; GENE FUSION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METHODOLOGY; MOLECULAR CLONING; PLASMID; SCHIZOSACCHAROMYCES; SITE DIRECTED MUTAGENESIS;

ARTIFICIAL GENE FUSION; CLONING, MOLECULAR; DNA REPAIR; GENETIC ENGINEERING; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SCHIZOSACCHAROMYCES;

SACCHAROMYCETALES; SCHIZOSACCHAROMYCETACEAE;

EID: 77950149296     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90967     Document Type: Article

References (18)

1. Cohen SN, Chang AY, Boyer HW, Helling RB Proc. Natl. Acad. Sci. USA 70 3240-3244 (1973).
2. Oldenburg KR, Vo KT, Michaelis S, and Paddon C, Nucleic Acids Res., 25, 451-452 (1997).
3. Hua S, Qiu M, Chan E, Zhu L, and Luo Y, Plasmid, 38, 91-96 (1997).
4. Iizasa E and Nagano Y, Biotechniques, 40, 79-83 (2006).
5. Marykwas DL and Passmore SE, Proc. Natl. Acad. Sci. USA, 92, 11701-11705 (1995).
6. Wang L, Kao R, Ivey FD, and Hoffman CS, Methods, 33, 199-205 (2004).
7. Kostrub CF, Lei EP, and Tamar E, Nucleic Acids Res., 26, 4783-4784 (1998).
8. Kaur R, Ingacale SS, and Bachhawat AK, Nucleic Acids Res., 25, 1080-1081 (1997).
9. Bähler J, Wu JQ, Longtine MS, Shah NG, McKenzie A3rd, Steever AB, Wach A, Philippsen P, and Pringle JR, Yeast, 14, 943-951 (1998).
10. Krawchuk MD and Wahls WP, Yeast, 15, 1419-1427 (1999).
11. Keeney JB and Boeke JD, Genetics, 136, 849-856 (1994).
12. Hashida-Okado T, Yasumoto R, Endo M, Takesako K, and Kato I, Curr. Genet., 33,38-45 (1998).
13. Maundrell K, J. Biol. Chem., 265, 10857-10864 (1990).
14. Moreno S, Klar A, and Nurse P, Methods Enzymol., 194, 795-823 (1991).
15. Matsuo Y, Asakawa K, Toda T, and Katayama S, Biosci. Biotechnol. Biochem., 70, 1992-1994 (2006).
16. Umehara T, Chimura T, Ichikawa N, and Horikoshi M, Genes Cells, 7, 59-73 (2002).
17. Basi G, Schmid E, and Maundrell K, Gene, 123, 131-136 (1993).
18. Miki R, Saiki R, Ozoe Y, and Kawamukai M, FEBS J., 275, 5309-5324 (2008).