Purification of recombinant plant-made proteins from corn extracts by ultrafiltration

Journal of Membrane Science

Volumn 353, Issue 1-2, 2010, Pages 103-110

  Aspelund, Matthew T ^a   Glatz, Charles E ^a  

^a Iowa State University of Science and Technology   (United States)

Author keywords

Collagen; Concentration polarization; Protein precipitation; Protein purification; Tangential flow filtration; Transgenic corn; Ultrafiltration

Indexed keywords

COLLAGEN CONCENTRATION; FLOW FILTRATION; PROTEIN PRECIPITATION; PROTEIN PURIFICATION; TANGENTIAL-FLOW FILTRATION; TRANSGENIC CORN;

COLLAGEN; PH EFFECTS; POLARIZATION; PURIFICATION; THERMOMECHANICAL PULPING PROCESS; ULTRAFILTRATION;

MICROFILTRATION;

COLLAGEN TYPE 1; CORN EXTRACT; GREEN FLUORESCENT PROTEIN; PLANT EXTRACT; POLYETHERSULFONE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CROSSFLOW FILTRATION; DIAFILTRATION; ENDOSPERM; FLOW RATE; MEMBRANE FILTER; MEMBRANE TRANSPORT; MOLECULAR WEIGHT; PH; POROSITY; PRECIPITATION; PRESSURE; PRIORITY JOURNAL; PROTEIN PURIFICATION; TRANSMEMBRANE PRESSURE; ULTRAFILTRATION;

EID: 77950088155     PISSN: 03767388     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.memsci.2010.02.036     Document Type: Article

References (27)

1. Zhang C., Baez J., Glatz C.E. Purification and characterization of a 44-kDa recombinant collagen I alpha 1 fragment from corn grain. J. Agric. Food Chem. 2009, 57:880-887.
2. Evangelista R.L., Kusnadi A.R., Howard J.A., Nikolov Z.L. Process and economic evaluation of the extraction and purification of recombinant beta-glucuronidase from transgenic corn. Biotechnol. Prog. 1998, 14:607-614.
3. Menkhaus T.J., Bai Y., Zhang C.M., Nikolov Z.L., Glatz C.E. Considerations for the recovery of recombinant proteins from plants. Biotechnol. Prog. 2004, 20:1001-1014.
4. Zhang C., Baez J., Glatz C.E. Purification and characterization of a transgenic corn grain-derived recombinant collagen type I alpha I. Biotechnol. Prog. 2009, 25:1660-1668.
5. Menkhaus T.J., Glatz C.E. Antibody capture from corn endosperm extracts by packed bed and expanded bed adsorption. Biotechnol. Prog. 2005, 21:473-485.
6. Menkhaus T.J., Glatz C.E. Compatibility of column inlet and adsorbent designs for processing of corn endosperm extract by expanded bed adsorption. Biotechnol. Bioeng. 2004, 87:324-336.
7. Gu Z., Glatz C.E. Aqueous two-phase extraction for protein recovery from corn extracts. J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci. 2007, 845:38-50.
8. Gehlert G., Luque S., Belfort G. Comparison of ultra- and microfiltration in the presence and absence of secondary flow with polysaccharides, proteins, and yeast suspensions. Biotechnol. Prog. 1998, 14:931-942.
9. Saxena A., Tripathi B.P., Kumar M., Shahi V.K. Membrane-based techniques for the separation and purification of proteins: an overview. Adv. Colloid Interf. Sci. 2009, 145:1-22.
10. van Reis R., Zydney A. Bioprocess membrane technology. J. Membr. Sci. 2007, 297:16-50.
11. van Reis R., Gadam S., Frautschy L.N., Orlando S., Goodrich E.M., Saksena S., Kuriyel R., Simpson C.M., Pearl S., Zydney A.L. High performance tangential flow filtration. Biotechnol. Bioeng. 1997, 56:71-82.
12. van Reis R., Brake J.M., Charkoudian J., Burns D.B., Zydney A.L. High-performance tangential flow filtration using charged membranes. J. Membr. Sci. 1999, 159:133-142.
13. Saksena S., Zydney A.L. Effect of solution ph and ionic-strength on the separation of albumin from immunoglobulins (IGG) by selective filtration. Biotechnol. Bioeng. 1994, 43:960-968.
14. Vaneijndhoven R., Saksena S., Zydney A.L. Protein fractionation using electrostatic interactions in membrane filtration. 7th Int. Conf. Recovery Biol. Prod. 1994.
15. vanReis R., Gadam S., Frautschy L.N., Orlando S., Goodrich E.M., Saksena S., Kuriyel R., Simpson C.M., Pearl S., Zydney A.L. High performance tangential flow filtration. Biotechnol. Bioeng. 1997, 56:71-82.
16. Baruah G.L., Nayak A., Winkelman E., Belfort G. Purification of monoclonal antibodies derived from transgenic goat milk by ultrafiltration. Biotechnol. Bioeng. 2006, 93:747-754.
17. Ibanez R., Guadix A., Guadix E., Almecija M. Effect of pH on the fractionation of whey proteins with a ceramic ultrafiltration membrane. J. Membr. Sci. 2007, 288:28-35.
18. vanReis R., Saksena S. Optimization diagram for membrane separations. J. Membr. Sci. 1997, 129:19-29.
19. Shepherd C.T., Vignaux N., Peterson J.M., Johnson L.A., Scott M.P. Green fluorescent protein as a tissue marker in transgenic maize seed. Cereal Chem. 2008, 85:188-195.
20. Vignaux N., Octaviani D., Johnson L. Efficiencies of different types of dry mills in recovering a fraction rich in recombinant protein expressed in endosperm. Annu. Meet. Am. Assoc. Cereal Chem. Tortilla Ind. Assoc. 2004.
21. Shepherd C.T., Vignaux N., Peterson J.M., Scott M.P., Johnson L.A. Dry-milling and fractionation of transgenic maize seed tissues with green fluorescent protein as a tissue marker. Cereal Chem. 2008, 85:196-201.
22. Shepherd C.T., Vignaux N., Peterson J.M., Scott M.P., Johnson L.A. Dry-milling and fractionation of transgenic maize seed tissues with green fluorescent protein as a tissue marker. Cereal Chem. 2008, 85:196.
23. Millipore, Pellicon XL Device Operating Instructions, Series Ed. 2001.
24. Cobb G.W. Introduction to Design and Analysis of Experiments 1998, Springer-Verlag, New York. Series Ed.
25. Zhong Q., Gu Z., Glatz C.E. Extraction of recombinant dog gastric lipase from transgenic corn seed. J. Agric. Food Chem. 2006, 54:8086-8092.
26. Zeugolis D.I., Li B., Lareu R.R., Chan C.K., Raghunath M. Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. J. Biomater. Sci. Polym. Ed. 2008, 19:1307-1317.
27. Gu Z.R., Glatz C.E. A method for three-dimensional protein characterization and its application to a complex plant (corn) extract. Biotechnol. Bioeng. 2007, 97:1158-1169.