Novel lines of Pax6-/- embryonic stem cells exhibit reduced neurogenic capacity without loss of viability

BMC Neuroscience

Volumn 11, Issue , 2010, Pages

  Quinn, Jane C ^a,b   Molinek, Michael ^a   Nowakowski, Tomasz J ^a   Mason, John O ^a   Price, David J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b CHARLES STURT UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR PAX6; EYE PROTEIN; HOMEODOMAIN PROTEIN; PAIRED BOX TRANSCRIPTION FACTOR; REPRESSOR PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL MUTANT; CELL SURVIVAL; CELL VIABILITY; CHIMERA; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; FEMALE; IN VITRO STUDY; IN VIVO STUDY; MALE; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE CELL NECROSIS; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; PHENOTYPE; ANIMAL; BRAIN CORTEX; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; GENETICS; HIPPOCAMPUS; METABOLISM; NERVE CELL; PHYSIOLOGY; TIME; TRANSGENIC MOUSE;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CEREBRAL CORTEX; CHIMERA; EMBRYONIC STEM CELLS; EYE PROTEINS; HIPPOCAMPUS; HOMEODOMAIN PROTEINS; MICE; MICE, TRANSGENIC; NEUROGENESIS; NEURONS; PAIRED BOX TRANSCRIPTION FACTORS; PHENOTYPE; REPRESSOR PROTEINS; TIME FACTORS;

EID: 77949890900     PISSN: None     EISSN: 14712202     Source Type: Journal    
DOI: 10.1186/1471-2202-11-26     Document Type: Article

References (33)

1. Walther C, Gruss P. Pax-6, a murine paired box gene, is expressed in the developing CNS. Development 1991, 113(4):1435-1449.
2. Stoykova A, Gruss P. Roles of Pax-genes in developing and adult brain as suggested by expression patterns. J Neurosci 1994, 14(3 Pt 2):1395-1412.
3. Pituello F, Yamada G, Gruss P. Activin A inhibits Pax-6 expression and perturbs cell differentiation in the developing spinal cord in vitro. Proc Natl Acad Sci USA 1995, 92(15):6952-6956. 10.1073/pnas.92.15.6952, 41449, 7624351.
4. Kioussi C, O'Connell S, St-Onge L, Treier M, Gleiberman AS, Gruss P, Rosenfeld MG. Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development. Proc Natl Acad Sci USA 1999, 96(25):14378-14382. 10.1073/pnas.96.25.14378, 24444, 10588713.
5. Engelkamp D, Rashbass P, Seawright A, van Heyningen V. Role of Pax6 in development of the cerebellar system. Development 1999, 126(16):3585-3596.
6. Hogan BL, Horsburgh G, Cohen J, Hetherington CM, Fisher G, Lyon MF. Small eyes (Sey): a homozygous lethal mutation on chromosome 2 which affects the differentiation of both lens and nasal placodes in the mouse. J Embryol Exp Morphol 1986, 97:95-110.
7. Hogan BL, Hirst EM, Horsburgh G, Hetherington CM. Small eye (Sey): a mouse model for the genetic analysis of craniofacial abnormalities. Development 1988, 103(Suppl):115-119.
8. Heins N, Malatesta P, Cecconi F, Nakafuku M, Tucker KL, Hack MA, Chapouton P, Barde YA, Gotz M. Glial cells generate neurons: the role of the transcription factor Pax6. Nat Neurosci 2002, 5(4):308-315. 10.1038/nn828, 11896398.
9. Quinn JC, Molinek M, Martynoga BS, Zaki PA, Faedo A, Bulfone A, Hevner RF, West JD, Price DJ. Pax6 controls cerebral cortical cell number by regulating exit from the cell cycle and specifies cortical cell identity by a cell autonomous mechanism. Dev Biol 2007, 302(1):50-65. 10.1016/j.ydbio.2006.08.035, 2384163,2384163, 16979618.
10. Pinson J, Simpson TI, Mason JO, Price DJ. Positive autoregulation of the transcription factor Pax6 in response to increased levels of either of its major isoforms, Pax6 or Pax6(5a), in cultured cells. BMC Dev Biol 2006, 6:25. 10.1186/1471-213X-6-25, 1489926, 16725027.
11. Nikoletopoulou V, Plachta N, Allen ND, Pinto L, Gotz M, Barde YA. Neurotrophin Receptor-Mediated Death of Misspecified Neurons Generated from Embryonic Stem Cells Lacking Pax6. Cell Stem Cell 2007, 1(5):529-540. 10.1016/j.stem.2007.08.011, 18371392.
12. Lee SH, Lumelsky N, Studer L, Auerbach JM, McKay RD. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat Biotechnol 2000, 18(6):675-679. 10.1038/76536, 10835609.
13. Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 2003, 21(2):183-186. 10.1038/nbt780, 12524553.
14. Bibel M, Richter J, Schrenk K, Tucker KL, Staiger V, Korte M, Goetz M, Barde YA. Differentiation of mouse embryonic stem cells into a defined neuronal lineage. Nat Neurosci 2004, 7(9):1003-1009. 10.1038/nn1301, 15332090.
15. Bibel M, Richter J, Lacroix E, Barde YA. Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells. Nat Protoc 2007, 2(5):1034-1043. 10.1038/nprot.2007.147, 17546008.
16. Watanabe K, Kamiya D, Nishiyama A, Katayama T, Nozaki S, Kawasaki H, Watanabe Y, Mizuseki K, Sasai Y. Directed differentiation of telencephalic precursors from embryonic stem cells. Nat Neurosci 2005, 8(3):288-296. 10.1038/nn1402, 15696161.
17. Gaspard N, Bouschet T, Hourez R, Dimidschstein J, Naeije G, Ameele J, Espuny-Camacho I, Herpoel A, Passante L, Schiffmann SN, et al. An intrinsic mechanism of corticogenesis from embryonic stem cells. Nature 2008, 455(7211):351-357. 10.1038/nature07287, 18716623.
18. Caric D, Gooday D, Hill RE, McConnell SK, Price DJ. Determination of the migratory capacity of embryonic cortical cells lacking the transcription factor Pax-6. Development 1997, 124(24):5087-5096.
19. Pinon MC, Tuoc TC, Ashery-Padan R, Molnar Z, Stoykova A. Altered molecular regionalization and normal thalamocortical connections in cortex-specific Pax6 knock-out mice. J Neurosci 2008, 28(35):8724-8734. 10.1523/JNEUROSCI.2565-08.2008, 18753373.
20. Quinn JC, West JD, Hill RE. Multiple functions for Pax6 in mouse eye and nasal development. Genes Dev 1996, 10(4):435-446. 10.1101/gad.10.4.435, 8600027.
21. Collinson JM, Quinn JC, Buchanan MA, Kaufman MH, Wedden SE, West JD, Hill RE. Primary defects in the lens underlie complex anterior segment abnormalities of the Pax6 heterozygous eye. Proc Natl Acad Sci USA 2001, 98(17):9688-9693. 10.1073/pnas.161144098, 55513, 11481423.
22. Talamillo A, Quinn JC, Collinson JM, Caric D, Price DJ, West JD, Hill RE. Pax6 regulates regional development and neuronal migration in the cerebral cortex. Dev Biol 2003, 255(1):151-163. 10.1016/S0012-1606(02)00046-5, 12618140.
23. Hill RE, Favor J, Hogan BL, Ton CC, Saunders GF, Hanson IM, Prosser J, Jordan T, Hastie ND, van Heyningen V. Mouse small eye results from mutations in a paired-like homeobox-containing gene. Nature 1991, 354(6354):522-525. 10.1038/354522a0, 1684639.
24. Lo CW, Coulling M, Kirby C. Tracking of mouse cell lineage using microinjected DNA sequences: analyses using genomic Southern blotting and tissue-section in situ hybridizations. Differentiation 1987, 35(1):37-44. 10.1111/j.1432-0436.1987.tb00149.x, 3428512.
25. Collinson JM, Quinn JC, Hill RE, West JD. The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo. Dev Biol 2003, 255(2):303-312. 10.1016/S0012-1606(02)00095-7, 12648492.
26. Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972, 26(4):239-257. 2008650, 4561027.
27. Lotto RB, Asavaritikrai P, Vali L, Price DJ. Target-derived neurotrophic factors regulate the death of developing forebrain neurons after a change in their trophic requirements. J Neurosci 2001, 21(11):3904-3910.
28. Turksen K. Embryonic Stem Cells, Methods and Protocols 2002, 185. Totowa, New Jersey: Humana Press.
29. Quinn JC, West JD, Kaufman MH. Genetic background effects on dental and other craniofacial abnormalities in homozygous small eye (Pax6Sey/Pax6Sey) mice. Anat Embryol (Berl) 1997, 196(4):311-321. 10.1007/s004290050100, 9363853.
30. Estivill-Torrus G, Pearson H, van Heyningen V, Price DJ, Rashbass P. Pax6 is required to regulate the cell cycle and the rate of progression from symmetrical to asymmetrical division in mammalian cortical progenitors. Development 2002, 129(2):455-466.
31. Philips GT, Stair CN, Young Lee H, Wroblewski E, Berberoglu MA, Brown NL, Mastick GS. Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type. Dev Biol 2005, 279(2):308-321. 10.1016/j.ydbio.2004.12.018, 15733660.
32. Bel-Vialar S, Medevielle F, Pituello F. The on/off of Pax6 controls the tempo of neuronal differentiation in the developing spinal cord. Dev Biol 2007, 305(2):659-673. 10.1016/j.ydbio.2007.02.012, 17399698.
33. Duparc RH, Abdouh M, David J, Lepine M, Tetreault N, Bernier G. Pax6 controls the proliferation rate of neuroepithelial progenitors from the mouse optic vesicle. Dev Biol 2007, 301(2):374-387. 10.1016/j.ydbio.2006.11.006, 17157287.