In vitro propagation of the endangered plant centaurea ultreiae: Assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis

Plant Cell, Tissue and Organ Culture

Volumn 101, Issue 1, 2010, Pages 31-39

  Mallón, Rubén ^a,b   Rodríguez Oubiña, Juan ^a   González, María Luz ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b Instituto de Investigaciones Agrobiológicas de Galicia del Consejo Superior de Investigaciones Científicas   (Spain)

Author keywords

Conservation; Flow cytometry; Germplasm; RAPD

Indexed keywords

6-BENZYLADENINE; CULTURE SYSTEMS; ENDANGERED PLANTS; ENVIRONMENTAL CONDITIONS; GENETIC STABILITY; GENOMIC ALTERATIONS; GERMPLASM; GERMPLASMS; IN-VITRO; MICROPROPAGATION; MULTIPLE SHOOTS; MULTIPLICATION RATE; MURASHIGE AND SKOOG MEDIUMS; NAPHTHALENEACETIC ACID; PLANT MATERIAL; RANDOM AMPLIFIED POLYMORPHIC DNAS; RAPD ANALYSIS; REGENERANTS;

CONSERVATION; FLOW CYTOMETRY; FORESTRY; GENES; SYSTEM STABILITY;

PLANTS (BOTANY);

CENTAUREA;

EID: 77949307811     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-009-9659-y     Document Type: Article

References (41)

1. Agarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant science. Plant Cell Rep 27: 617-631. doi: 10. 1007/s00299-008-0507-z.
2. Arumuganathan K, Earle ED (1991) Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Biol Rep 9: 229-233. doi: 10. 1007/BF02672073.
3. Bairu MW, Fennell CW, van Staden J (2006) The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. 'Zelig'). Sci Hort 108: 347-351. doi: 10. 1016/j. scienta. 2006. 01. 039.
4. Bais HP, Green JB, Walker TS, Okemo PO, Vivanco JM (2002) In vitro propagation of Spilanthes mauritiana DC., an endangered medicinal herb, through axillary bud cultures. In Vitro Cell Dev Biol-Plant 38: 598-601. doi: 10. 1079/IVP2002345.
5. Bañares Á, Blanca G, Güemes J, Moreno JC, Ortiz S (eds) (2003) Atlas y libro rojo de la flora vascular amenazada de España. Dirección General de Conservación de la Naturaleza, Madrid.
6. Bennett MD, Price HJ, Johnston JS (2008) Anthocyanin inhibits propidium iodide DNA fluorescence in Euphorbia pulcherrima: implications for genome size variation and flow cytometry. Ann Bot 101: 777-790. doi: 10. 1093/aob/mcm303.
7. Borthakur M, Dutta K, Nath SC, Singh RS (2000) Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique. Plant Cell Tiss Org Cult 62: 239-242. doi: 10. 1023/A: 1006465517666.
8. Brutti C, Apóstolo NM, Ferrarotti SA, Llorente BE, Krymkiewicz N (2000) Micropropagation of Cynara scolymus L. employing cyclodextrins to promote rhizogenesis. Sci Hort 83: 1-10. doi: 10. 1016/S0304-4238(99)00067-9.
9. Clemente M (1991) The micropropagation unit at the Cordoba Botanic Garden, Spain. Botanic Gardens Micropropagation News 1: 30-32.
10. Clemente M, Contreras P, Susin J, Pliego-Alfaro F (1991) Micropropagation of Artemisia granatensis. HortSci 26: 420.
11. Cuenca S, Amo-Marco JB (2000) In vitro propagation of Centaurea spachii from inflorescence stems. Plant Growth Regul 30: 99-103. doi: 10. 1023/A: 1006356811180.
12. Cuenca S, Amo-Marco JB, Parra R (1999) Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae). Plant Cell Rep 18: 674-679. doi: 10. 1007/s002990050641.
13. Doležel J, Binarová P, Lucretti S (1989) Analysis of nuclear DNA content in plant cells flow cytometry. Biol Plant 31: 113-120.
14. Estades J, Medrano H (1990) Contribución a la conservación de plantas endémicas de Baleares mediante cultivo in vitro. In: Hernández-Bermejo JE, Clemente M, Heywood V (eds) Conservation techniques in botanic gardens. Koeltz Scientific Books, Koenigstein, pp 121-123.
15. George EF (1993) Plant propagation by tissue culture. Exegetics Limited, Edington.
16. González C, Rubio A, Ortega C (1989) Propagación in vitro de endemismos canarios en peligro de extinción: Atractylis arbuscula Svent. Et Michaelis. Bot Macaronesica 17: 47-56.
17. Goto S, Thakur RC, Ishii K (1998) Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers. Plant Cell Rep 18: 193-197. doi: 10. 1007/s002990050555.
18. Guo WL, Gong L, Ding ZF, Li YD, Li FX, Zhao SP, Liu B (2006) Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. F., as revealed by ISSR and RAPDs markers. Plant Cell Rep 25: 896-906. doi: 10. 1007/s00299-006-0131-8.
19. Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26: 261-265. doi: 10. 1007/s00299-006-0230-6.
20. Hashmi G, Huettel R, Meyer R, Krusberg L, Hammerschlag F (1997) RAPD analysis of somaclonal variants derived from embryo callus cultures of peach. Plant Cell Rep 16: 624-627. doi: 10. 1007/BF01275503.
21. Hossain A, Konisho M, Minami K, Nemoto K (2003) Somaclonal variation of regenerated plants in chili pepper (Capsicum annuum L.). Euphytica 130: 233-239. doi: 10. 1023/A: 1022856725794.
22. Howell SH, Lall S, Che P (2003) Cytokinins and shoot development. Trends Plant Sci 8: 453-459. doi: 10. 1016/S1360-1385(03)00191-2.
23. Hummer KE (1999) Biotechnology in plant germplasm acquisition. In: Benson EE (ed) Plant Conservation Biotechnology. Taylor & Francis, London, pp 25-40.
24. Jensen WA (1962) Botanical histochemistry. Freeman and Company, San Francisco.
25. Kashif Husain M, Anis M (2006) Rapid in vitro propagation of Eclipta alba (L) Hassk by shoot tip culture. J Plant Biochem Biotech 15: 147-179.
26. Lanham PG, Brenner RM (1999) Genetic characterisation of gooseberry (Ribes grossularia subgenus Grossularia) germplasm using RAPD, ISSR and AFLP markers. J Hortic Sci Biol 74: 361-366.
27. Larkin PJ, Scowcroft WR (1981) Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197-214.
28. Loureiro J, Rodriguez E, Doležel J, Santos C (2006) Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Ann Bot 98: 679-689. doi: 10. 1093/aob/mcl141.
29. Lowe AJ, Hanotte O, Guarino L (1996) Standardization of molecular genetic techniques for the characterization of germplasm collections: the case of random amplified polymorphic DNA (RAPD). Plant Gen Res NewsLetter 107: 50-54.
30. Mallón R, Bunn E, Turner SR, González ML (2008) Cryopreservation of Centaurea ultreiae (Compositae) a critically endangered species from Galicia (Spain). CryoLetters 29: 363-370.
31. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473-497. doi: 10. 1111/j. 1399-3054. 1962. tb08052. x.
32. Ortega C, González C (1985) Contribución a la conservación ex situ de especies canarias en peligro: propagación in vitro de Senecio hermosae Pitard. Bot Macaronesica 14: 59-72.
33. Otto F (1992) Preparation and staining of cells for high-resolution DNA analysis. In: Radbruch A (ed) Flow cytometry and cell sorting. Springer, Berlin, pp 101-104.
34. Ozel CA, Khawar KM, Mirici S, Ozcan S, Arslan O (2006) Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey). Naturwissenschaften 93: 511-517. doi: 10. 1007/s00114-006-0139-5.
35. Palombi MA, Damiano C (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep 20: 1061-1066. doi: 10. 1007/s00299-001-0430-z.
36. Pfosser M, Amon A, Lelley T, Heberle-Bors E (1995) Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21: 387-393.
37. Price HJ, Morgan PW, Johnston S (1998) Environmentally correlated variation in 2C nuclear DNA content measurements in Helianthus annuus L. Ann Bot 82: 95-98.
38. Radić S, Prolić M, Pavlica M, Pevalek-Kozlina B (2005) Cytogenetic stability of Centaurea ragusina long-term culture. Plant Cell Tiss Org Cult 82: 343-348. doi: 10. 1007/s11240-005-2388-y.
39. Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Prendergast G, Rowntree JK (2006) Conservation in vitro of threatened plants-Progress in the past decade. In Vitro Cell Dev Biol-Plant 42: 206-214. doi: 10. 1079/IVP2006769.
40. Sujatha G, Ranjitha Kumari BD (2007) Effect of phytohormones on micropropagation of Artemisia vulgaris L. Acta Physiol Plant 29: 189-195. doi: 10. 1007/s11738-006-0023-0.
41. Valladares S, Sánchez C, Martínez MT, Ballester A, Vieitez AM (2006) Plant regeneration through somatic embryogenesis from tissues of mature oak trees: tru-to-type conformity of plantlets by RAPD analysis. Plant Cell Rep 25: 879-886. doi: 10. 1007/s00299-005-0108-z.