Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): A multifunctional protein essential for spermatogenesis

Journal of Andrology

Volumn 31, Issue 1, 2010, Pages 45-52

  Tsai Morris, Chon Hwa ^a   Sheng, Yi ^a   Gutti, Ravi K ^a   Tang, Pei Zhong ^a   Dufau, Maria L ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

Apoptosis; Chromatoid body; Infertility; Messenger ribonuclear protein particle; Missense mutation; Phosphorylation; Shuttling protein; Single nucleotide polymorphism (SNP); Spermiogenesis; Testis; Translation

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; ANDROGEN; ANDROSTANOLONE; CHORIONIC GONADOTROPIN; CYCLIC AMP; DEAD BOX PROTEIN; FLUTAMIDE; GONADOTROPIN; GONADOTROPIN REGULATED TESTICULAR RNA HELICASE; HELICASE; MESSENGER RNA; RIBONUCLEOPROTEIN; RNA HELICASE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANDROGEN RELEASE; APOPTOSIS; AUTOCRINE EFFECT; CELL MATURATION; CONFERENCE PAPER; CONTRACEPTION; CYTOPLASM; ENZYME ACTIVITY; ENZYME BINDING; ENZYME DEFICIENCY; GENE CONTROL; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC CONSERVATION; GENETIC TRANSCRIPTION; GERM CELL; GROWTH DISORDER; KNOCKOUT GENE; KNOCKOUT MOUSE; LEYDIG CELL; MALE; MALE INFERTILITY; MISSENSE MUTATION; MOUSE; NONHUMAN; NUCLEUS ACCUMBENS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; RNA BINDING; RNA METABOLISM; RNA STRUCTURE; RNA TRANSLATION; SERTOLI CELL; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; TRANSCRIPTION REGULATION; TUMOR CELL; UPREGULATION; WILD TYPE;

ANIMALS; DEAD-BOX RNA HELICASES; GENE EXPRESSION REGULATION; HUMANS; MALE; MICE; ORGAN SPECIFICITY; SPERMATOGENESIS; TESTIS;

EID: 77749252218     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: 10.2164/jandrol.109.008219     Document Type: Conference Paper

References (35)

1. Catt KJ, Harwood JP, Clayton RN, Davies TF, Chan V, Katikineni M, Nozu K, Dufau ML. Regulation of peptide hormone receptors and gonadal steroidogenesis. Recent Prog Harm Res. 1980;36: 557-662.
2. Chang C, Chen YT, Yeh SD, Xu Q, Wang RS, Guillou F, Lardy H, Yeh S. Infertility with defective spermatogenesis and hypotestosteronemia in male mice lacking the androgen receptor in Sertoli cells. Proc Natl Acad Sci U S A. 2004;101:6876-6881.
3. Chuang RY, Weaver PL, Liu Z, Chang TH. Requirement of the DEAD-Box protein ded1p for messenger RNA translation. Science. 1997;275:1468-1471.
4. Cordin O, Banroques J, Tanner NK, Linder P. The DEAD-box protein family of RNA helicases. Gene. 2006;67:17-37.
5. de la Cruz J, Kressler D, Linder P. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci. 1999;24:192-198.
6. Donovan M, Cotter TG. Control of mitochondrial integrity by Bc1-2 family members and caspase-independent cell death. Biochim Biophys Acta. 2004;1644:133-147.
7. Dufau ML. Endocrine regulation and communicating functions of the Leydig cell. Annu Rev Physiol. 1988;50:483-508.
8. Dufau ML, Tsai-Morris CH. Gonadotropin-regulated testicular helicase (GRTH/DDX25): an essential regulator of spermatogenesis. Trends Endocrinol Metab. 2007;18:314-320.
9. Dufau ML, Tsuruhara T, Horner KA, Podesta E, Catt KJ. Intermediate role of adenosine 3′:5′-cyclic monophosphate and protein kinase during gonadotropin-induced steroidogenesis in testicular interstitial cells. Proc Natl Acad Sci U S A. 1977;74: 3419-3423.
10. Eddy EM. Male germ cell gene expression. Recent Prog Harm Res. 2002;57:103-128.
11. Eulalio A, Behm-Ansmant I, Izaurralde E. P bodies: at the crossroads of post-transcriptional pathways. Nat Rev Mol Cell Biol. 2007;8:9-22.
12. Fujiwara Y, Komiya T, Kawabata H, Sato M, Fujimoto H, Furusawa M, Noce T. Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage. Proc Natl Acad Sci U S A. 1994;91:12258-12262.
13. Gee SL, Conboy JG. Mouse erythroid cells express multiple putative RNA helicase genes exhibiting high sequence conservation from yeast to mammals. Gene. 1994;140:171-177.
14. Gutti RK, Tsai-Morris CH, Dufau ML. Gonadotropin-regulated testicular helicase (DDX25), an essential regulator of spermatogenesis, prevents testicular germ cell apoptosis. J Biol Chem. 2008;283:17055-17064.
15. Hecht NB. Haploid gene expression and the regulation of post-meiotic structural genes. In: Parvinen M, Huhtaniemi I, & Pelliniemi LJ eds. Development and Function of the Reproductive Organs. Rome, Italy: Ares-Serono Symposia; 1988;325-334.
16. Holdcraft RW, Braun RE. Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids. Development. 2004;131459-167.
17. Kotaja N, Sassone-Corsi P. The chromatoid body: a germ-cell-specific RNA-processing centre. Nat Rev Mol Cell Biol. 2007;8:85-90.
18. Leroy P, Alzari P, Sassoon D, Wolgemuth D, Fellous M. The protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicase. Cell. 1989;57:549-559.
19. Linder P. Dead-box proteins: a family affair-active and passive players in RNP-remodeling. Nucleic Acids Res. 2006;34:4168-4180.
20. Noce T, Okamoto-Ito S, Tsunekawa N. Vasa homolog genes in mammalian germ cell development. Cell Struct Funct. 2001;26: 131-136.
21. Parvinen M. The chromatoid body in spermatogenesis, lnt J Androl. 2005;28:189-201.
22. Parvinen M, Parvinen LM. Active movements of the chromatoid body. A possible transport mechanism for haploid gene products. J Cell Biol. 1979;80:621-628.
23. Sheng Y, Tsai-Morris CH, Dufau ML. Cell-specific and hormone-regulated expression of gonadotropin-regulated testicular RNA helicase gene (GRTH/DDX25) resulting from alternative utilization of translation initiation codons in the rat testis. J Biol Chem. 2003;278:27796-27803.
24. Sheng Y, Tsai-Morris CH, Gutti R, Maeda Y, Dufau ML. Gonadotropin- regulated testicular RNA helicase (GRTH/ DDX25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis. J Biol Chem. 2006;281:35048-35056.
25. Silverman E, Edwalds-Gilbert G, Lin RJ. DExD/H-box proteins and their partners: helping RNA helicases unwind. Gene. 2003;312: 1-16.
26. Steger K. Haploid spermatids exhibit translationally repressed mRNAs. Anat Embryol! (Berl). 2001;203:323-334.
27. Tanaka SS, Toyooka Y, Akasu R, Katoh-Fukui Y, Nakahara Y, Suzuki R, Yokoyama M, Noce T. The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000;14:841-853.
28. Tang PZ, Tsai-Morris CH, Dufau ML. A novel gonadotropin-regulated testicular RNA helicase. A new member of the dead-box family. J Biol Chem. 1999;274:37932-37940.
29. Tsai-Morris CH, Koh E, Dufau ML. Differences in gonadotropin-regulated testicular helicase (GRTH/DDX25) single nucleotide polymorphism between Japanese and Chinese populations. Hum Reprod. 2008;23:2611-2613.
30. Tsai-Morris CH, Koh E, Sheng Y, Maeda Y, Gutti R, Namiki M, Dufau ML. Polymorphism of the GRTH/DDX25 gene in normal and infertile Japanese men: a missense mutation associated with loss of GRTH phosphorylation. Mol Hum Reprod. 2007;13: 887-892.
31. Tsai-Morris CH, Lei S, Jiang Q, Sheng Y, Dufau ML. Genomic organization and transcriptional analysis of gonadotropin-regulated testicular RNA helicase-GRTH/DDX25 gene. Gene. 2004a; 331:83-94.
32. Tsai-Morris CH, Sheng Y, Lee E, Lei KJ, Dufau ML. Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) is essential for spermatid development and completion of spermatogenesis. Proc Natl Acad Sci U S A. 2004b;101:6373-6378.
33. Tseng SS, Weaver PL, Liu Y, Hitomi M, Tartakoff AM, Chang TH. Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export. EMBO J. 1998;17:2651-2662.
34. Wang RS, Yeh S, Chen LM, Lin HY, Zhang C, Ni J, Wu CC, di Sant'Agnese PA, deMesy-Bentley KL, Tzeng CR, Chang C. Androgen receptor in Sertoli cell is essential for germ cell nursery and junctional complex formation in mouse testes. Endocrinology. 2006;147:5624-5633.
35. Zhoucun A, Zhang S, Yang Y, Ma Y, Lin L, Zhang W. Single nucleotide polymorphisms of the gonadotrophin-regulated testicular helicase (GRTH) gene may be associated with the human spermatogenesis impairment. Hum Reprod. 2006;21: 755-759.