Metabolic turnover analysis by a combination of in vivo 13C-labelling from 13CO2 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C3 photosynthetic pathway in Nicotiana tabacum leaves

Journal of Experimental Botany

Volumn 61, Issue 4, 2010, Pages 1041-1051

  Hasunuma, Tomohisa ^a,c   Harada, Kazuo ^b   Miyazawa, Shin Ichi ^a   Kondo, Akihiko ^c   Fukusaki, Eiichiro ^b   Miyake, Chikahiro ^a,c  

^a RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c KOBE UNIVERSITY   (Japan)

Author keywords

13CO2; Capillary electrophoresis tandem mass spectrometry (CE MS MS); In vivo labelling; Metabolic turnover; Photosynthesis; Sugar phosphate

Indexed keywords

CARBON; CARBON DIOXIDE; GLUCOSE 1 PHOSPHATE; GLUCOSE 6 PHOSPHATE; GLUCOSE PHOSPHATE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; EVALUATION; ISOTOPE LABELING; KINETICS; METABOLISM; METHODOLOGY; PHOTOSYNTHESIS; PLANT LEAF; TANDEM MASS SPECTROMETRY; TOBACCO;

CARBON DIOXIDE; CARBON ISOTOPES; ELECTROPHORESIS, CAPILLARY; GLUCOSE-6-PHOSPHATE; GLUCOSEPHOSPHATES; ISOTOPE LABELING; KINETICS; PHOTOSYNTHESIS; PLANT LEAVES; TANDEM MASS SPECTROMETRY; TOBACCO;

EMBRYOPHYTA; NICOTIANA TABACUM;

EID: 77649255304     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erp374     Document Type: Article

References (39)

1. Baroja-Fernández E, Muñoz FJ, Akazawa T, Pozueta-Romero J. 2001. Reappraisal of the currently prevailing model of starch biosynthesis in photosynthetic tissues: a proposal involving the cytosolic production of ADP-glucose by sucrose synthase and occurrence of cyclic turnover of starch in the chloroplast. Plant and Cell Physiology 42, 1311-1320.
2. 2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase and the rate of respiration in the light. Planta 165, 397-406.
3. Cruz JA, Emery C, Wüst M, Kramer DM, Lange BM. 2008. Metabolic profiling of Calvin cycle intermediates by HPLC-MS using mixed-mode stationary phases. The Plant Journal 55, 1047-1060.
4. 2 is fed to intact leaves. Plant, Cell and Environment 16, 587-591.
5. Dennis DT, Blakeley SD. 2000. Carbohydrate metabolism. In: Buchanan W, Gruissem R, Jones R, eds. The biochemistry and molecular biology of plants. Rockville, MD: American Society of Plant Physiologists, 630-675.
6. 3 species. Planta 149, 78-90.
7. Farquhar GD, von Caemmerer S, Berry JA. 2001. Models of photosynthesis. Plant Physiology 125, 42-45.
8. Fiehn O, Kopka J, Dörmann P, Altmann T, Trethewey RN, Willmitzer L. 2000. Metabolite profiling for plant functional genomics. Nature Biotechnology 18, 1157-1161.
9. Giersch C. 2000. Mathematical modelling of metabolism. Current Opinion in Plant Biology 3, 249-253.
10. Haake V, Zrenner R, Sonnewald U, Stitt M. 1998. A moderate decrease of plastid aldolase activity inhibits photosynthesis, alters the levels of sugars and starch, and inhibits growth of potato plants. The Plant Journal 14, 147-157.
11. Harada K, Ohyama Y, Tabushi T, Kobayashi A, Fukusaki E. 2008. Quantitative analysis of anionic metabolites for Catharanthus roseus by capillary electrophoresis using sulfonated capillary coupled with electrospray ionization-tandem mass spectrometry. Journal of Bioscience and Bioengineering 105, 249-260.
12. Harrison EP, Willingham NM, Lloyd JC, Raines CA. 1998. Reduced sedoheptulose-1,7-bisphosphatase levels in transgenic tobacco lead to decreased photosynthetic capacity and altered carbohydrate accumulation. Planta 204, 27-36.
13. Harrison EP, Olcxer H, Lloyd JC, Long SP, Raines CA. 2001. Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate, but do not affect Rubisco carboxylation capacity. Journal of Experimental Botany 52, 1779-1784.
14. Henkes S, Sonnewald U, Badur R, Flachmann R, Stitt M. 2001. A small decrease of plastidial transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and polypropanoid metabolism. The Plant Cell 13, 535-551.
15. Huber SC, Hanson KR. 1992. Carbon partitioning and growth of a starchless mutant of Nicotiana sylvestris. Plant Physiology 99, 1449-1454.
16. 2 labelling. Phytochemistry 68, 2258-2272.
17. Koch K. 2004. Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Current Opinion in Plant Biology 7, 235-246.
18. Koßmann J, Sonnewald U, Willmitzer L. 1994. Reduction of the chloroplastic fructose-1,6-bisphosphatase in transgenic potato plants impairs photosynthesis and plant growth. The Plant Journal 6, 637-650.
19. Kruckeberg AL, Neuhaus HE, Feil R, Gottlieb LD, Stitt M. 1989. Decreased-activity mutants of phosphoglucose isomerase in the cytosol and chloroplast of Clarkia xantiana. Biochemical Journal 261, 457-467.
20. Lytovchenko A, Sweetlove L, Pauly M, Fernie AR. 2002a. The influence of cytosolic phosphoglucomutase on photosynthetic carbohydrate metabolism. Planta 215, 1013-1021.
21. Lytovchenko A, Bieberich K, Willmitzer L, Fernie AR. 2002b. Carbon assimilation and metabolism in potato leaves deficient in plastidial phosphoglucomutase. Planta 215, 802-811.
22. Lytovchenko A, Hajirezaei M, Eickmeier I, Mittendorf V, Sonnewald U, Willmitzer L, Fernie AR. 2005. Expression of an Escherichia coli phosphoglucomutase in potato (Solanum tuberosum L.) results in minor changes in tuber metabolism and a considerable delay in tuber sprouting. Planta 221, 915-927.
23. Ölcxer H, Lloyd JC, Raines CA. 2001. Photosynthetic capacity is differentially affected by reductions in sedoheptulose-1,7-bisphosphatase activity during leaf development in transgenic tobacco plants. Plant Physiology 125, 982-989.
24. 2 assimilation and growth in low irradiance. The Plant Journal 7, 535-542.
25. Periappuram C, Steinhauer L, Barton DL, Taylor DC, Chatson B, Zou J. 2000. The plastidic phosphoglucomutase from Arabidopsis. A reversible enzyme reaction with an important role in metabolic control. Plant Physiology 122, 1193-1200.
26. Poolman MG, Fell DA, Thomas S. 2000. Modelling photosynthesis and its control. Journal of Experimental Botany 51, 319-328.
27. 2 assimilation via a reduction in ribulose bisphosphate regeneration in transgenic tobacco plants. Planta 195, 369-378.
28. Roessner U, Wagner C, Kopka J, Trethewey RN, Willmitzer L. 2000. Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. The Plant Journal 23, 131-142.
29. Rolleston FS. 1972. A theoretical background to the use of measured intermediates in the study of the control of intermediary metabolism. Current Topics in Cellular Regulation 5, 47-75.
30. Sato S, Soga T, Nishioka T, Tomita M. 2004. Simultaneous determination of the metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection system. The Plant Journal 40, 151-163.
31. Schwender J. 2008. Metabolic flux analysis as a tool in metabolic engineering of plants. Current Opinion in Biotechnology 19, 131-137.
32. 3 plants: physics, physiology and rate limitations. The Botanical Review 51, 53-105.
33. 2 and light in Phaseolus vulgaris. Plant Physiology 81, 788-791.
34. Tamoi M, Nagaoka M, Yabuta Y, Shigeoka S. 2005. Carbon metabolism in the Calvin cycle. Plant Biotechnology 22, 355-360.
35. van der Straeten D, Rodrigues-Pousada RA, Goodman HM, Van Montagu M. 1991. Plant enolase: gene structure, expression, and evolution. The Plant Cell 3, 719-735.
36. Weise SE, Schrader SM, Kleinbeck KR, Sharkey TD. 2006. Carbon balance and circadian regulation of hydrolytic and phosphorolytic breakdown of transitory starch. Plant Physiology 141, 879-886.
37. Winter H, Robinson DG, Heldt HW. 1994. Subcellular volumes and metabolite concentrations in spinach leaves. Planta 193, 530-535.
38. Zeeman SC, Smith SM, Smith AM. 2007. The diurnal metabolism of leaf starch. Biochemical Journal 401, 13-28.
39. Zhu X-G, de Sturler E, Long SP. 2007. Optimizing the distribution of resources between enzymes of carbon metabolism can dramatically increase photosynthetic rate: a numerical simulation using an evolutionary algorithm. Plant Physiology 145, 513-526.