Platelet-derived growth factor receptor signaling is not involved in osteogenic differentiation of human mesenchymal stem cells

Tissue Engineering - Part A

Volumn 16, Issue 3, 2010, Pages 983-993

  Kumar, Akhilesh ^a,b   Salimath, Bharathi P ^b   Stark, Gerhard Björn ^a   Finkenzeller, Günter ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF MYSORE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; BLOOD SUBSTITUTES; CALCIUM; CELL CULTURE; ENZYME ACTIVITY; FLOWCHARTING; OSTEOBLASTS; PEPTIDES; PHOSPHATASES; PHOSPHORYLATION; PLATELETS; SIGNALING; STEM CELLS; TRANSCRIPTION; TRANSCRIPTION FACTORS; WELL STIMULATION;

ALKALINE PHOSPHATASE; BONE SIALOPROTEINS; COLLAGEN TYPE I; CONCENTRATION-DEPENDENT; EXTRACELLULAR-REGULATED KINASE; FETAL CALF SERUMS; HUMAN MESENCHYMAL STEM CELLS; MARKER GENES; MATRIX; MESENCHYMAL STEM CELL; MRNA EXPRESSION; OSTEOBLASTIC DIFFERENTIATION; OSTEOCALCIN; OSTEOGENESIS; OSTEOGENIC; OSTEOGENIC DIFFERENTIATION; OSTEOPONTIN; PLATELET-DERIVED GROWTH FACTOR RECEPTORS; PLATELET-DERIVED GROWTH FACTORS; RECEPTOR ACTIVITY; RECEPTOR SIGNALING; RECEPTOR STIMULATION; SPECIFIC INHIBITORS; TYRPHOSTIN;

EXPERIMENTS;

ALKALINE PHOSPHATASE; BIOLOGICAL MARKER; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; OSTEOCALCIN; PLATELET DERIVED GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR BB; PLATELET DERIVED GROWTH FACTOR RECEPTOR; PLATELET-DERIVED GROWTH FACTOR BB;

AGED; ARTICLE; BONE DEVELOPMENT; BONE MATRIX; CELL DIFFERENTIATION; CELL PROLIFERATION; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; FEMALE; GENE EXPRESSION REGULATION; HUMAN; MALE; MESENCHYMAL STEM CELL; METABOLISM; MIDDLE AGED; PHOSPHORYLATION; SIGNAL TRANSDUCTION;

AGED; ALKALINE PHOSPHATASE; BIOLOGICAL MARKERS; BONE MATRIX; CELL DIFFERENTIATION; CELL PROLIFERATION; FEMALE; GENE EXPRESSION REGULATION; HUMANS; MALE; MESENCHYMAL STEM CELLS; MIDDLE AGED; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; OSTEOCALCIN; OSTEOGENESIS; PHOSPHORYLATION; PLATELET-DERIVED GROWTH FACTOR; RECEPTORS, PLATELET-DERIVED GROWTH FACTOR; SIGNAL TRANSDUCTION;

EID: 77649250577     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0230     Document Type: Article

References (32)

1. Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., and Marshak, D.R. Multilineage potential of adult human mesenchymal stem cells. Science 284, 143, 1999.
2. Beyer Nardi, N., and da Silva Meirelles, L. Mesenchymal stem cells: isolation, in vitro expansion and characterization. Handb Exp Pharmacol 174, 249, 2006.
3. Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, CD., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T., Lund, T., Blackstad, M., Du, J., Aldrich, S., Lisberg, A., Low, W.C., Largaespada, D.A., and Verfaillie, CM. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418, 41, 2002.
4. Smith, J.R., Pochampally, R., Perry, A., Hsu, S.C, and Prockop, D.J. Isolation of a highly clonogenic and multipo-tential subfraction of adult stem cells from bone marrow stroma. Stem Cells 22, 823, 2004.
5. Lazarus, H.M., Haynesworth, S.E., Gerson, S.L., Rosenthal, N.S., and Caplan, A.I. Ex vivo expansion and subsequent infusion of human bone marrow-derived stromal progenitor cells (mesenchymal progenitor cells): implications for therapeutic use. Bone Marrow Transplant 16, 557, 1995.
6. Mooney, D.J., and Mikos, A.G. Growing new organs. Sci Am 280, 60, 1999.
7. Bruder, S.P., Jaiswal, N., and Haynesworth, S.E. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive sub-cultivation and following cryopreservation. J Cell Biochem 64, 278, 1997.
8. Datta, N., Holtorf, H.L., Sikavitsas, V.I., Jansen, J.A., and Mikos, A.G. Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells. Biomaterials 26, 971, 2005.
9. Nakagami, H., Morishita, R., Maeda, K., Kikuchi, Y., Ogi-hara, T., and Kaneda, Y. Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy. J Atheroscler Thromb 13, 77, 2006.
10. Chunmeng, S., and Tianmin, C. Skin: a promising reservoir for adult stem cell populations. Med Hypotheses 62, 683, 2004.
11. Lee, R.H., Kim, B., Choi, I., Kim, H., Choi, H.S., Suh, K., Bae, Y.C., and Jung, J.S. Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 14, 311, 2004.
12. Conget, P.A., and Minguell, J.J. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 181, 67, 1999.
13. Hock, J.M., and Canalis, E. Platelet-derived growth factor enhances bone cell replication, but not differentiated function of osteoblasts. Endocrinology 134, 1423, 1994.
14. Kubota, K., Sakikawa, C, Katsumata, M., Nakamura, T., and Wakabayashi, K. Platelet-derived growth factor BB secreted from osteoclasts acts as an osteoblastogenesis inhibitory factor. J Bone Miner Res 17, 257, 2002.
15. Fitter, S., Dewar, A.L., Kostakis, P., To, L.B., Hughes, T.P., Roberts, M.M., Lynch, K., Vernon-Roberts, B., and Zannet-tino, A.C. Long-term imatinib therapy promotes bone formation in CML patients. Blood 111, 2538, 2008.
16. O'Sullivan, S., Naot, D., Callon, K., Porteous, F., Home, A., Wattie, D., Watson, M., Cornish, J., Browett, P., and Grey, A. Imatinib promotes osteoblast differentiation by inhibiting PDGFR signaling and inhibits osteoclastogenesis by both direct and stromal cell-dependent mechanisms. J Bone Miner Res 22, 1679, 2007.
17. Ponte, A.L., Marais, E., Gallay, N., Langonne, A., Delorme, B., Herault, O., Charbord, P., and Domenech, J. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells 25, 1737, 2007.
18. Fierro, F., Illmer, T., Jing, D., Schleyer, E., Ehninger, G., Boxberger, S., and Bornhauser, M. Inhibition of platelet-derived growth factor receptorbeta by imatinib mesylate suppresses proliferation and alters differentiation of human mesenchymal stem cells in vitro. Cell Prolif 40, 355, 2007.
19. Fiedler, J., Etzel, N., and Brenner, R.E. To go or not to go: migration of human mesenchymal progenitor cells stimulated by isoforms of PDGF. J Cell Biochem 93, 990, 2004.
20. Kovalenko, M., Gazit, A., Bohmer, A., Rorsman, C, Ronn-strand, L., Heldin, C.H., Waltenberger, J., Bohmer, F.D., and Levitzki, A. Selective platelet-derived growth factor receptor kinase blockers reverse sis-transformation. Cancer Res 54, 6106, 1994.
21. Matthews, W., Jordan, C.T., Wiegand, G.W., Pardoll, D., and Lemischka, I.R. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 65, 1143, 1991.
22. Mehlhorn, A.T., Schmal, H., Kaiser, S., Lepski, G., Finken-zeller, G., Stark, G.B., and Sudkamp, N.P. Mesenchymal stem cells maintain TGF-beta-mediated chondrogenic phe-notype in alginate bead culture. Tissue Eng 12, 1393, 2006.
23. Blum, J.S., Li, R.H., Mikos, A.G., and Barry, M.A. An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2. J Cell Biochem 80, 532, 2001.
24. Medhurst, A.D., Harrison, D.C., Read, S.J., Campbell, C.A., Robbins, M.J., and Pangalos, M.N. The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models. J Neurosci Methods 98, 9, 2000.
25. Yu, X., Hsieh, S.C., Bao, W., and Graves, D.T. Temporal expression of PDGF receptors and PDGF regulatory effects on osteoblastic cells in mineralizing cultures. Am J Physiol 272, CI 709, 1997.
26. Tokunaga, A., Oya, T., Ishii, Y., Motomura, H., Nakamura, C, Ishizawa, S., Fujimori, T., Nabeshima, Y., Umezawa, A., Kanamori, M., Kimura, T., and Sasahara, M. PDGF receptor beta is a potent regulator of mesenchymal stromal cell function. J Bone Miner Res 23, 1519, 2008.
27. Ng, F., Boucher, S., Koh, S., Sastry, K.S., Chase, L., Laksh-mipathy, U., Choong, C, Yang, Z., Vemuri, M.C., Rao, M.S., and Tanavde, V. PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages. Blood 112, 295, 2008.
28. Ross, R., Glomset, J., Kariya, B., and Harker, L. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci USA 71, 1207, 1974.
29. Benitz, W.E., Lessler, D.S., Coulson, J.D., and Bernfield, M. Heparin inhibits proliferation of fetal vascular smooth muscle cells in the absence of platelet-derived growth factor. J Cell Physiol 127, 1, 1986.
30. Ilmer, M., Karow, M., Geissler, C, Jochum, M., and Neth, P. Human osteoblast-derived factors induce early osteogenic markers in human mesenchymal stem cells. Tissue Eng Part A 15, 2397, 2009.
31. Qi, H., Aguiar, D.J., Williams, S.M., La Pean, A., Pan, W., and Verfaillie, CM. Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells. Proc Natl Acad Sci USA 100, 3305, 2003.
32. Kratchmarova, I., Blagoev, B., Haack-Sorensen, M., Kassem, M., and Mann, M. Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation. Science 308, 1472, 2005