Engineering a pharmacologically superior form of granulocyte-colony-stimulating factor by fusion with gelatin-like-protein polymer

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 74, Issue 3, 2010, Pages 435-441

  Huang, Yan Shan ^a   Wen, Xiao Fang ^a   Wu, Yi Liang ^a   Wang, Ye Fei ^a   Fan, Min ^a   Yang, Zhi Yu ^a   Liu, Wei ^a   Zhou, Lin Fu ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Fusion protein; Gelatin; Granulocyte colony stimulating factor; Half life; Pichia pastoris

Indexed keywords

GELATIN LIKE PROTEIN POLYMER; GRANULOCYTE COLONY STIMULATING FACTOR; POLYMER; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BIOENGINEERING; CHIMERA; DRUG ADMINISTRATION ROUTE; DRUG BLOOD LEVEL; DRUG CLEARANCE; DRUG ELIMINATION; DRUG HALF LIFE; DRUG PROTEIN BINDING; EXPERIMENTAL RAT; FEMALE; HEMATOPOIESIS; HYDRODYNAMICS; HYDROPHILICITY; IN VITRO STUDY; LEUKOCYTE COUNT; MOUSE; NEUTROPHIL; NONHUMAN; PICHIA PASTORIS; POLYMERIZATION; PROTEIN PURIFICATION; RAT; THERMOSTABILITY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY FORMATION; BASE SEQUENCE; CELL LINE, TUMOR; CELL PROLIFERATION; DRUG STABILITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GELATIN; GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; HALF-LIFE; HUMANS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PICHIA; POLYMERS; PROTEIN ENGINEERING; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFECTION;

EID: 77649189678     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2009.12.002     Document Type: Article

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