Analysis of branched-chain α-keto acid dehydrogenase complex activity in rat tissues using α-keto[1-13C]isocaproate as substrate

Analytical Biochemistry

Volumn 399, Issue 1, 2010, Pages 1-6

  Matsumoto, Hideki ^a   Akita, Keiichi ^b   Sakai, Ryosei ^a   Shimomura, Yoshiharu ^c  

^a AJINOMOTO CO INC   (Japan)

^b NAGOYA INSTITUTE OF TECHNOLOGY   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

Branched chain amino acid; Branched chain keto acid dehydrogenase complex; Isotope ratio mass spectrometry; Ketoisocaproate

Indexed keywords

ENZYMES; GAS CHROMATOGRAPHY; HISTOLOGY; ISOTOPES; MASS SPECTROMETRY; MOLAR RATIO; POTASH; RATS; SPECTROMETERS;

BRANCHED CHAIN AMINO ACIDS; DEHYDROGENASE COMPLEXES; GAS CHROMATOGRAPHY-ISOTOPE-RATIO MASS SPECTROMETRIES; INTERNAL STANDARDS; ISOTOPE RATIO MASS SPECTROMETRY; KETO ACIDS; LINEAR RELATIONSHIPS; RADIOCHEMICAL METHOD;

MUSCLE;

2 OXOISOCAPROIC ACID; 2 OXOISOVALERATE DEHYDROGENASE (LIPOAMIDE); CARBON DIOXIDE; POTASSIUM CARBONATE;

ACIDIFICATION; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GAS CHROMATOGRAPHY; ISOTOPE RATIO MASS SPECTROMETRY; MALE; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RADIOCHEMISTRY; RAT; SENSITIVITY AND SPECIFICITY; SKELETAL MUSCLE;

RATTUS;

EID: 77649188637     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.12.017     Document Type: Article

References (15)

1. Harper A.E., Miller R.H., Block K.P. Branched-chain amino acid metabolism. Annu. Rev. Nutr. 1984, 4:409-454.
2. Shimomura Y., Obayashi M., Murakami T., Harris R.A. Regulation of branched-chain amino acid catabolism: nutritional and hormonal regulation of activity and expression of the branched-chain α-keto acid dehydrogenase kinase. Curr. Opin. Clin. Nutr. Metab. Care 2001, 4:419-423.
3. Paxton R., Harris R.A. Isolation of rabbit liver branched chain α-keto acid dehydrogenase and regulation by phosphorylation. J. Biol. Chem. 1982, 257:14433-14439.
4. Shimomura Y., Honda T., Shiraki M., Murakami T., Sato J., Kobayashi H., Mawatari K., Obayashi M., Harris R.A. Branched-chain amino acid catabolism in exercise and liver disease. J. Nutr. 2006, 136:250S-253S.
5. Harris R.A., Zhang B., Goodwin G.W., Kuntz M.J., Shimomura Y., Rougraff P., Dexter P., Zhao Y., Gibson R., Crabb D.W. Regulation of the branched-chain α-keto acid dehydrogenase and elucidation of a molecular basis for maple syrup urine disease. Adv. Enzyme Regul. 1990, 30:245-263.
6. Shimomura Y., Fujii H., Suzuki M., Murakami T., Fujitsuka N., Nakai N. Branched-chain α-keto acid dehydrogenase complex in rat skeletal muscle: regulation of the activity and gene expression by nutrition and physical exercise. J. Nutr. 1995, 125:1762S-1765S.
7. Suryawan A., Hawes J.W., Harris R.A., Shimomura Y., Jenkins A.E., Hutson S.M. A molecular model of human branched-chain amino acid metabolism. Am. J. Clin. Nutr. 1998, 68:72-81.
8. Paul H.S., Adibi S.A. Leucine oxidation and protein turnover in clofibrate-induced muscle protein degradation in rats. J. Clin. Invest. 1980, 65:1285-1293.
9. Nakai N., Kobayashi R., Popov K.M., Harris R.A., Shimomura Y. Determination of branched-chain α-keto acid dehydrogenase activity state and branched-chain α-keto acid dehydrogenase kinase activity and protein in mammalian tissue. Methods Enzymol. 2000, 324:48-62.
10. Shimomura Y., Suzuki T., Saitoh S., Tasaki Y., Harris R.A., Suzuki M. Activation of branched-chain α-keto acid dehydrogenase complex by exercise: effect of high-fat diet intake. J. Appl. Physiol. 1990, 68:161-165.
11. 2 analysis. Gut 1998, 43:S2-S6.
12. Reeves P.G., Nielsen F.H., Fahey G.C. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 1993, 123:1939-1951.
13. Detection limit, in: The Japanese Pharmacopoeia, 15th ed. (JPXV), Ministry of Health, Labor, and Welfare, 2008, p. 1649 (Japanese version) or 1761 (English version).
14. DeSantiago S., Torres N., Suryawan A., Tovar A.R., Hutson S.M. Regulation of branched-chain amino acid metabolism in the lactating rat. J. Nutr. 1998, 128:1165-1171.
15. Block K.P., Soemitro S., Heywood B.W., Harper A.E. Activation of liver branched-chain α-keto acid dehydrogenase in rats by excesses of dietary amino acids. J. Nutr. 1985, 115:1550-1561.