Application of glutaraldehyde to in-cell Western assay for normalization

Analytical Biochemistry

Volumn 398, Issue 2, 2010, Pages 254-256

  Li, Jingbao ^a   Wang, Zhe ^a   He, Gang ^a   Zhang, Wei ^a   Qian, Airong ^a   Shang, Peng ^a  

^a NORTHWESTERN POLYTECHNICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; INFRARED DEVICES;

CELL NUMBER; GLUTARALDEHYDES; NEAR INFRARED; NEAR INFRARED REGION; PROTEIN ANALYSIS; REDUCED COST; SIMPLIFIED PROCEDURE;

CELLS;

GLUTARALDEHYDE;

ARTICLE; CELL ASSAY; CELL COUNT; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN CELL WESTERN ASSAY; NEAR INFRARED SPECTROSCOPY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

EID: 77649186110     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.12.013     Document Type: Article

References (13)

1. Theiss A.L., Simmons J.G., Jobin C., Lund P.K. Tumor necrosis factor (TNF) alpha increases collagen accumulation and proliferation in intestinal myofibroblasts via TNF receptor 2. J. Biol. Chem. 2005, 280:36099-36109.
2. Bouzeghrane F., Reinhardt D.P., Reudelhuber T.L., Thibault G. Enhanced expression of fibrillin-1, a constituent of the myocardial extracellular matrix in fibrosis. Am. J. Physiol. Heart Circ. Physiol. 2005, 289:H982-H991.
3. Dickey C.A., Dunmore J., Lu B., Wang J.W., Lee W.C., Kamal A., Burrows F., Eckman C., Hutton M., Petrucelli L. HSP induction mediates selective clearance of tau phosphorylated at proline-directed Ser/Thr sites but not KXGS (MARK) sites. FASEB J. 2006, 20:753-755.
4. Feliers D., Gorin Y., Ghosh-Choudhury G., Abboud H.E., Kasinath B.S. Angiotensin II stimulation of VEGF mRNA translation requires production of reactive oxygen species. Am. J. Physiol. Renal Physiol. 2006, 290:F927-F936.
5. Kovar J.L., Volcheck W.M., Chen J., Simpson M.A. Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice. Anal. Biochem. 2007, 361:47-54.
6. Chen H., Kovar J., Sissons S., Cox K., Matter W., Chadwell F., Luan P., Vlahos C.J., Schutz-Geschwender A., Olive D.M. A cell-based immunocytochemical assay for monitoring kinase signaling pathways and drug efficacy. Anal. Biochem. 2005, 338:136-142.
7. Gonzalez Bosc L.V., Layne J.J., Nelson M.T., Hill-Eubanks D.C. Nuclear factor of activated T cells and serum response factor cooperatively regulate the activity of an α-actin intronic enhancer. J. Biol. Chem. 2005, 280:26113-26120.
8. Hannoush R.N. Kinetics of Wnt-driven β-catenin stabilization revealed by quantitative and temporal imaging. PLoS ONE 2008, 3:e3498.
9. Godin-Heymann N., Ulkus L., Brannigan B.W., McDermott U., Lamb J., Maheswaran S., Settleman J., Haber D.A. The T790M " gatekeeper" mutation in EGFR mediates resistance to low concentrations of an irreversible EGFR inhibitor. Mol. Cancer Ther. 2008, 7:874-879.
10. Sabatini D.D., Bensch K., Barrnett R.J. Cytochemistry and electron microscopy: the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 1963, 17:19-58.
11. Biberfeld P., Biberfeld G., Molnar Z., Fagraeus A. Fixation of cell-bound antibody in the membrane immunofluorescence test. J. Immunol. Methods 1974, 4:135-148.
12. Frank R.D., Dresbach H., Thelen H., Sieberth H.G. Glutardialdehyde induced fluorescence technique (GIFT): a new method for the imaging of platelet adhesion on biomaterials. J. Biomed. Mater. Res. 2000, 52:374-381.
13. Collins J.S., Goldsmith T.H. Spectral properties of fluorescence induced by glutaraldehyde fixation. J. Histochem. Cytochem. 1981, 29:411-414.