Synthesis and characterization of a cellular membrane affinity chromatography column containing histamine 1 and P2Y1 receptors: A multiple G-protein coupled receptor column

Journal of Pharmaceutical and Biomedical Analysis

Volumn 52, Issue 3, 2010, Pages 416-419

  Moaddel, Ruin ^a   Musyimi, Harrison K ^a   Sanghvi, Mitesh ^a   Bashore, Charlene ^a   Frazier, Chester R ^a   Khadeer, Mohammad ^a   Bhatia, Prateek ^a   Wainer, Irving W ^a  

^a NATIONAL INSTITUTE ON AGING   (United States)

Author keywords

Affinity chromatography; Anti histamines; Drug discovery; Membrane receptors; Receptor screening

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; HISTAMINE H1 RECEPTOR; MEPYRAMINE; PURINERGIC P2Y1 RECEPTOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELLULAR MEMBRANE AFFINITY CHROMATOGRAPHY; CONTROLLED STUDY; HUMAN; HUMAN CELL; LIGAND BINDING; MEMBRANE BINDING; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING;

ASTROCYTOMA; CELL LINE, TUMOR; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; HUMANS; KINETICS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, HISTAMINE; RECEPTORS, PURINERGIC P2; TRANSFECTION;

EID: 77649183824     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2009.06.035     Document Type: Article

References (11)

1. Moaddel R., and Wainer I.W. Development of immobilized membrane-based affinity columns for use in the online characterization of membrane bound proteins and for targeted affinity isolations. Anal. Chim. Acta 546 (2006) 97-105
2. Moaddel R., and Wainer I.W. The preparation and development of cellular membrane affinity chromatography columns. Nat. Protoc. 4 (2009) 197-205
3. Marinissen M.J., and Gutkind J.S. G-protein-coupled receptors and signaling networks: emerging paradigms. Trends Pharmacol. Sci. 22 (2001) 368-375
4. Beigi F., and Wainer I.W. Syntheses of immobilized G protein-coupled receptor chromatographic stationary phases: characterization of immobilized μ and κ opioid receptors. Anal. Chem. 75 (2003) 4480-4485
5. Beigi F., Chakir K., Xiao R., and Wainer I.W. G-Protein-coupled receptor chromatographic stationary phases. 2. Ligand-induced conformational mobility in an immobilized β2-adrenergic receptor. Anal. Chem. 76 (2004) 7187-7193
6. 1) liquid chromatography stationary phase. Anal. Biochem. 364 (2007) 216-218
7. Temporini C., Ceruti S., Calleri E., Ferrario S., Moaddel R., Abbracchio M.P., and Massolini G. Development of an immobilized GRP17 receptor stationary phase for binding determination using frontal affinity chromatography coupled to mass spectrometry. Anal. Biochem. 384 (2009) 123-129
8. Katabatake T., Moaddel R., Cole R., Gandhari M., Frazier C., Hartenstein J., Rosenberg A., Bernier M., and Wainer I.W. Characterization of a multiple ligand-gated ion channel cellular membrane affinity chromatography column and identification of endogenously expressed receptors in astrocytoma cell lines. Anal. Chem. 80 (2008) 8673-8680
9. 1-Histamine receptors on human astrocytoma cells. Mol. Pharmacol. 29 (1986) 188-195
10. 2Y1 receptor. Br. J. Phamacol. 118 (1996) 167-173
11. 4 receptor in autoimmune disease. Expert Opin. Investig. Drugs 15 (2006) 1443-1452