Isolating intact chloroplasts from small Arabidopsis samples for proteomic studies

Analytical Biochemistry

Volumn 398, Issue 2, 2010, Pages 198-202

  Kley, Jeannette ^a   Heil, Martin ^b   Muck, Alexander ^a   Svatoš, Aleš ^a   Boland, Wilhelm ^a  

^a MAX PLANCK INSTITUTE FOR CHEMICAL ECOLOGY   (Germany)

^b DEPARTAMENTO DE INGENIERÍA GENÉTICA   (Mexico)

Author keywords

Arabidopsis thaliana; Biotic stress; Chloroplast; Proteomics

Indexed keywords

PLANTS (BOTANY); PROTEOMICS;

ARABIDOPSIS PLANT; ARABIDOPSIS THALIANA; BIOTIC STRESS; CHLOROPLAST; CHLOROPLAST PROTEINS; PLANT PATHOGEN INTERACTION; PROTEIN RECOVERY; PROTEOMIC STUDIES;

PROTEINS;

CELL PROTEIN;

ARABIDOPSIS; ARTICLE; BIOTIC STRESS; CELL ISOLATION; CHLOROPLAST; CONTROLLED STUDY; ENZYME ASSAY; GEL; IMMUNOASSAY; NONHUMAN; PLANT LEAF; PLANT PATHOGEN INTERACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; PURIFICATION; QUALITY CONTROL; SAMPLING;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 77649179118     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.11.016     Document Type: Article

References (36)

1. Biochemistry and Molecular Biology of Plants 2000, American Society of Plant Physiologists, Rockville, MD. B.B. Buchanan, W. Gruissem, R.L. Jones (Eds.).
2. Strawn M.A., Marr S.K., Inoue K., Inada N., Zubieta C., Wildermuth M.C. Arabidopsis isochorismate synthase functional in pathogen-induced salicylate biosynthesis exhibits properties consistent with a role in diverse stress responses. J. Biol. Chem. 2007, 282:5919-5933.
3. Wasternack C. Jasmonates: an update on biosynthesis, signal transduction, and action in plant stress response, growth, and development. Ann. Bot. 2007, 100:681-697.
4. Heil M., Ton J. Long-distance signalling in plant defence. Trends Plant Sci. 2008, 13:264-272.
5. Howe G.A., Jander G. Plant immunity to insect herbivores. Annu. Rev. Plant Biol. 2008, 59:41-66.
6. Loake G., Grant M. Salicylic acid in plant defence. The players and protagonists. Curr. Opin. Plant Biol. 2007, 10:466-472.
7. Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 2005, 43:205-227.
8. Kunst L. Preparation of physiologically active chloroplasts from Arabidopsis. Arabidopsis Protocols 1998, 43-48. Humana Press, Totowa, NJ. J.M. Martínez-Zapater, J. Salinas (Eds.).
9. Somerville C.R., Somerville S.C., Ogren W.L. Isolation of photosynthetically active protoplasts and chloroplasts from Arabidopsis thaliana. Plant Sci. Lett. 1981, 21:89-96.
10. Piel J., Atzorn R., Gabler R., Kuhnemann F., Boland W. Cellulysin from the plant parasitic fungus Trichoderma viride elicits volatile biosynthesis in higher plants via the octadecanoid signalling cascade. FEBS Lett. 1997, 416:143-148.
11. Heinemeyer J., Eubel H., Wehmhöner D., Jänsch L., Braun H.-P. Proteomic approach to characterize the supramolecular organization of photosystems in higher plants. Phytochemistry 2004, 65:1683-1692.
12. Kieselbach T., Bystedt M., Hynds P., Robinson C., Schröder W.P. A peroxidase homologue and novel plastocyanin located by proteomics to the Arabidopsis chloroplast thylakoid lumen. FEBS Lett. 2000, 480:271-276.
13. Salvi D., Rolland N., Joyard J., Ferro M. Purification and proteomic analysis of chloroplasts and their sub-organellar compartments. Organelle Proteomics 2008, 19-36. Humana Press, Totowa, NJ. D. Pflieger, J. Rossier (Eds.).
14. Goulas E., Schubert M., Kieselbach T., Kleczkowski L.A., Gardeström P., Schröder W., Hurry V. The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature. Plant J. 2006, 47:720-734.
15. Aronsson H., Jarvis P. A simple method for isolating import-competent Arabidopsis chloroplasts. FEBS Lett. 2002, 529:215-220.
16. Kubis S.E., Lilley K.S., Jarvis P. Isolation and preparation of chloroplasts from Arabidopsis thaliana plants. 2D PAGE: Sample Preparation and Fractionation 2008, 171-186. Humana Press, Totowa, NJ. A. Posch (Ed.).
17. Dietrich R., Ploss K., Heil M. Constitutive and induced resistance to pathogens in Arabidopsis thaliana depends on nitrogen supply. Plant Cell Environ. 2004, 27:896-906.
18. van Wijk K.J., Peltier J.-B., Giacomelli L. Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis. Plant Proteomics 2007, 43-48. Humana Press, Totowa, NJ. H. Thiellement, M. Zivy, C. Damerval, V. Méchin (Eds.).
19. Zrenner R., Willmitzer L., Sonnewald U. Analysis of the expression of potato uridinediphosphate-glucose pyrophosphorylase and its inhibition by antisense RNA. Planta 1993, 190:247-252.
20. Ramagli L.S., Rodriguez L.V. Quantitation of microgram amounts of protein in two-dimensional polyacrylamide-gel electrophoresis sample buffer. Electrophoresis 1985, 6:559-563.
21. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature 1970, 227:680-685.
22. Rehm H. Der Experimentator: Proteinbiochemie/Proteomics 2006, Elsevier, München, Germany.
23. Gonzalez-Teuber M., Eilmus S., Muck A., Svatos A., Heil M. Pathogenesis-related proteins protect extrafloral nectar from microbial infestation. Plant J. 2009, 58:464-473.
24. Salvi D., Rolland N., Joyard J., Ferro M. Assessment of organelle purity using antibodies and specific assays. Organelle Proteomics 2008, 345-356. Humana Press, Totowa, NJ. D. Pflieger, J. Rossier (Eds.).
25. Niedow J.N., Day D.A. Respiration and photorespiration. Biochemistry and Molecular Biology of Plants 2000, 676-728. American Society of Plant Physiologists, Rockville, MD. B.B. Buchanan, W. Gruissem, R.L. Jones (Eds.).
26. Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J. The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts. Mol. Cell. Proteomics 2006, 5:114-133.
27. Dennis D.T., Blakeley S.D. Carbohydrate metabolism. Biochemistry and Molecular Biology of Plants 2000, 630-675. American Society of Plant Physiologists, Rockville, MD. B.B. Buchanan, W. Gruissem, R.L. Jones (Eds.).
28. D. Seigneurin-Berny, D. Salvi, J. Joyard, N. Rolland, Purification of intact chloroplasts from Arabidopsis and spinach leaves by isopycnic centrifugation, Curr. Protoc. Cell Biol. (2008) unit 3.30.
29. Friso G., Giacomelli L., Ytterberg A.J., Peltier J.-B., Rudella A., Sun Q., van Wijk K.J. In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts: new proteins, new functions, and a plastid proteome database. Plant Cell 2004, 16:478-499.
30. Zybailov B., van Wijk K. Sorting signals, N-terminal modifications, and abundance of the chloroplast proteome. PLoS ONE 2008, 3:e1994.
31. Seigneurin-Berny D., Salvi D., Dorne A.-J., Joyard J., Rolland N. Percoll-purified and photosynthetically active chloroplasts from Arabidopsis thaliana leaves. Plant Physiol. Biochem. 2008, 46:951-955.
32. Reumann S., Babujee L., Ma C.L., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Luder F., Weckwerth W., Jahn O. Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms. Plant Cell 2007, 19:3170-3193.
33. Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjolander K., Gruissem W., Baginsky S. The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions. Curr. Biol. 2004, 14:354-362.
34. Kissen R., Rossiter J.T., Bones A.M. The " mustard oil bomb": not so easy to assemble?! Localization, expression and distribution of the components of the myrosinase enzyme system. Phytochem. Rev. 2009, 8:69-86.
35. Spremulli L. Protein synthesis, assembly, and degradation. Biochemistry and Molecular Biology of Plants 2000, 412-454. American Society of Plant Physiologists, Rockville, MD. B.B. Buchanan, W. Gruissem, R.L. Jones (Eds.).
36. Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana. Mol. Cell. Proteomics 2003, 2:325-345.