Mechanical behavior of human mesenchymal stem cells during adipogenic and osteogenic differentiation

Biochemical and Biophysical Research Communications

Volumn 393, Issue 1, 2010, Pages 150-155

  Yu, Haiyang ^a   Tay, Chor Yong ^a   Leong, Wen Shing ^a   Tan, Samuel Chun Wei ^b   Liao, Kin ^b   Tan, Lay Poh ^a  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b Nanyang Technological University   (Singapore)

Author keywords

Cellular mechanics; Cytoskeleton development; Differentiation; hMSCs; Micropipette aspiration

Indexed keywords

FILAMIN A; GAMMA TUBULIN; VIMENTIN;

ADIPOGENESIS; ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; CELL SHAPE; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOSKELETON; GENE EXPRESSION; HOMEOSTASIS; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MESENCHYMAL STEM CELL; MICROPIPETTE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; UPREGULATION; VISCOELASTICITY; YOUNG MODULUS;

ADIPOGENESIS; CELL LINEAGE; CONTRACTILE PROTEINS; ELASTIC MODULUS; GENE EXPRESSION; HUMANS; MESENCHYMAL STEM CELLS; MICROFILAMENT PROTEINS; OSTEOGENESIS; TISSUE ENGINEERING; TUBULIN;

EID: 77549088787     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.01.107     Document Type: Article

References (29)

1. Ingber D.E. Mechanobiology and diseases of mechanotransduction. Annals of Medicine 35 (2003) 564-577
2. Ingber D.E. Cellular mechanotransduction: putting all the pieces together again. The FASEB Journal 20 (2006) 811-827
3. Darling E.M., Topel M., Zauscher S., Vail T.P., and Guilak F. Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes. Journal of Biomechanics 41 (2008) 454-464
4. Jones W.R., Ting-Beall H.P., Lee G.M., Kelley S.S., Hochmuth R.M., and Guilak F. Alterations in the Young's modulus and volumetric properties of chondrocytes isolated from normal and osteoarthritic human cartilage. Journal of Biomechanics 32 (1999) 119-128
5. Bao G., and Suresh S. Cell and molecular mechanics of biological materials. Nature Materials 2 (2003) 715-725
6. P. Wenxiao, E. Petersen, C. Ning, M. Gang, L. Jian Run. F. Zhiqin, L. Kin, K.W. Leong, Viscoelastic properties of human mesenchymal stem cells, in: 27th Annual International Conference of the Engineering in Medicine and Biology Society, 2005 IEEE-EMBS 2005, pp. 4854-4857.
7. Trickey W.R., Baaijens F.P.T., Laursen T.A., Alexopoulos L.G., and Guilak F. Determination of the Poisson's ratio of the cell: recovery properties of chondrocytes after release from complete micropipette aspiration. Journal of Biomechanics 39 (2006) 78-87
8. Thoumine O., Cardoso O., and Meister J.J. Changes in the mechanical properties of fibroblasts during spreading: a micromanipulation study. European Biophysics Journal 28 (1999) 222-234
9. Sato M., Ohshima N., and Nerem R.M. Viscoelastic properties of cultured porcine aortic endothelial cells exposed to shear stress. Journal of Biomechanics 29 (1996) 461-467
10. Pablo Rodriguez M.G.S.R.V.C.J. Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation. Journal of Cellular Biochemistry 93 (2004) 721-731
11. Titushkin I., and Cho M. Modulation of cellular mechanics during osteogenic differentiation of human mesenchymal stem cells. Biophysical Journal 93 (2007) 3693-3702
12. R.L. Reger, A.H. Tucker, M.R. Wolfe, Differentiation and Characterization of Human MSCs, Mesenchymal Stem Cells, Humana Press, 2008, pp. 93-107.
13. Tan S., Pan W., Ma G., Cai N., Leong K., and Liao K. Viscoelastic behaviour of human mesenchymal stem cells. BMC Cell Biology 9 (2008) 40
14. Theret D.P., Levesque M.J., Sato M., Nerem R.M., and Wheeler L.T. The application of a homogeneous half-space model in the analysis of endothelial-cell micropipette measurements. Journal Biomechanical Engineering 110 (1988) 190-199
15. Tay C.Y., Gu H., Leong W.S., Yu H., Li H.Q., Heng B.C., Tantang H., Loo S.C.J., Li L.J., and Tan L.P. Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film. Carbon 48 (2010) 1095-1104
16. Wang X., and Seed B. A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Research 31 (2003) e154
17. Darling E.M., Zauscher S., Block J.A., and Guilak F. A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: do cell properties reflect metastatic potential?. Biophysical Journal 92 (2007) 1784-1791
18. Glogauer M., Arora P., Chou D., Janmey P.A., Downey G.P., and McCulloch C.A.G. The role of actin-binding protein 280 in integrin-dependent mechanoprotection. Journal of Biological Chemistry 273 (1998) 1689-1698
19. Zheng Y., Jung M.K., and Oakley B.R. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65 (1991) 817-823
20. Li Q., and Joshi H.C. Gamma-tubulin is a minus end-specific microtubule binding protein. Journal of Cell Biology 131 (1995) 207-214
21. Fuchs E., and Weber K. Intermediate filaments: structure, dynamics, function and disease. Annual Review of Biochemistry 63 (1994) 345-382
22. Ivaska J., Pallari H.M., Nevo J., and Eriksson J.E. Novel functions of vimentin in cell adhesion, migration, and signaling. Experimental Cell Research 313 (2007) 2050-2062
23. McBeath R., Pirone D.M., Nelson C.M., Bhadriraju K., and Chen C.S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Developmental Cell 6 (2004) 483-495
24. Thoumine O., Cardoso O., and Meister J.J. Changes in the mechanical properties of fibroblasts during spreading: a micromanipulation study. European Biophysics Journal with Biophysics Letters 28 (1999) 222-234
25. Badley R.A., Woods A., Carruthers L., and Rees D.A. Cytoskeleton changes in fibroblast adhesion and detachment. Journal of Cell Science 43 (1980) 379-390
26. Tandon R., Levental I., Huang C., Byfield F.J., Ziembicki J., Schelling J.R., Bruggeman L.A., Sedor J.R., Janmey P.A., and Miller R.T. HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties. American Journal of Physiology - Renal Physiology 292 (2007) F701-F710
27. Wang N., Naruse K., Stamenović D., Fredberg J.J., Mijailovich S.M., Tolić-Nørrelykke I.M., Polte T., Mannix R., and Ingber D.E. Mechanical behavior in living cells consistent with the tensegrity model. Proceedings of the National Academy of Sciences of the United States of America 98 (2001) 7765-7770
28. Goldman R.D., Khuon S., Chou Y.H., Opal P., and Steinert P.M. The function of intermediate filaments in cell shape and cytoskeletal integrity. Journal of Cell Biology 134 (1996) 971-983
29. Meyers V.E., Zayzafoon M., Douglas J.T., and McDonald J.M. RhoA and cytoskeletal disruption mediate reduced osteoblastogenesis and enhanced adipogenesis of human mesenchymal stem cells in modeled microgravity. Journal of Bone and Mineral Research 20 (2005) 1858-1866