The use of flow cytometry for species identification and life-cycle studies in dinoflagellates

Deep-Sea Research Part II: Topical Studies in Oceanography

Volumn 57, Issue 3-4, 2010, Pages 301-307

  Figueroa, Rosa Isabel ^a   Garces E ^a   Bravo, I ^b  

^a INSTITUT DE CIÈNCIES DEL MAR   (Spain)

^b INSTITUTO ESPAÑOL DE OCEANOGRAFÍA   (Spain)

Author keywords

Alexandrium; DNA content; Flow cytometry; Karlodinium; Sexuality

Indexed keywords

ALEXANDRIUM; ALEXANDRIUM MINUTUM; ALEXANDRIUM OSTENFELDII; ALEXANDRIUM TAMARENSE; CYTOMETRIC ANALYSIS; CYTOMETRY; DNA CONTENT; FLOW-CYTOMETRIC ANALYSIS; MORPHOLOGICAL CHARACTERISTIC; SPECIES IDENTIFICATION;

BLOOMS (METAL); CELL CULTURE; FLOW CYTOMETRY; GENES;

DNA;

DINOFLAGELLATE; DNA; FLOW CYTOMETRY; GENETIC ANALYSIS; IDENTIFICATION METHOD; LIFE CYCLE ANALYSIS; NOMENCLATURE; SEXUALITY;

ALEXANDRIUM; ALEXANDRIUM CATENELLA; ALEXANDRIUM MINUTUM; ALEXANDRIUM OSTENFELDII; ALEXANDRIUM PERUVIANUM; ALEXANDRIUM TAMARENSE; ALEXANDRIUM TAMUTUM; ARMIGER; DINOPHYCEAE; KARLODINIUM;

EID: 77549086478     PISSN: 09670645     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dsr2.2009.09.008     Document Type: Article

References (40)

1. Bolch, C.J.S., 1999. Evolution, species resolution and molecular population genetics of the Gymnodinium catenatum toxic dinoflagellate species complex: tracing global dispersal and population dynamics. Ph.D. Thesis, University of Tasmania, Australia. 199pp.
2. Blackburn S.I., Bolch C.J.S., Haskard K.A., and Hallegraeff G.M. Reproductive compatibility among four global populations of the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae). Phycologia 40 1 (2001) 78-87
3. Chisholm S.W., Olson R.J., Zettler E.R., Goericke R., Waterbury J.B., and Welshmeyer N.A. A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature 334 (1988) 340-343
4. Doolittle W.F., and Sapienza C. Selfish genes, the phenotype paradigm and genome evolution. Nature 284 (1980) 601-603
5. Ellegaard M., Kulis D.M., and Anderson D.M. Cysts of Danish Gymnodinium nolleri Ellegaard et Moestrup sp. ined. (Dinophyceae): studies on encystment, excystment and toxicity. Journal of Plankton Research 20 9 (1998) 1743-1755
6. Figueroa R.I., and Bravo I. A study of the sexual reproduction and determination of mating type of Gymnodinium nolleri (Dinophyceae) in culture. Journal of Phycology 41 1 (2005) 74-83
7. Figueroa R.I., Bravo I., and Garcés E. The multiple routes of sexuality in Alexandrium taylori (Dinophyceae) in culture. Journal of Phycology 42 5 (2006) 1028-1039
8. Figueroa R.I., Garcés E., and Bravo I. Comparative study between the life cycles of Alexandrium tamutum and Alexandrium minutum (Gonyaulacales, Dinophyceae) in culture. Journal of Phycology 43 5 (2007) 1039-1053
9. Figueroa R.I., Bravo E., and Garcés E. The significance of sexual versus asexual cyst formation in the life cycle of the noxious dinoflagellate Alexandrium peruvianum. Harmful Algae 7 5 (2008) 653-663
10. Garcés E., Bravo I., Vila M., Figueroa R., Masó M., and Sampedro N. Relationship between vegetative cells and cyst production during Alexandrium minutum bloom in Arenys de Mar harbour (NW Mediterranean). Journal of Plankton Research 26 6 (2004) 637-645
11. Giacobbe M.G., and Yang X. The life history of Alexandrium taylori (Dinophyceae). Journal of Phycology 35 2 (1999) 331-338
12. Guillard R.R.L., and Hargraves P.E. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia 32 (1993) 234-236
13. Gregory T.R. The C-value enigma in plants and animals: a review of parallels and an appeal for partnership. Annals of Botany 95 (2005) 133-146
14. Greilhuber J. Intraspecific variation in genome size: a critical reassessment. Annals of Botany 82 Sppl.A (1998) 27-35
15. Greilhuber J., Doležel J., Lysák M.A., and Bennett M.D. The origin, evolution and proposed stabilization of the term "genome size" and "C-value" to describe nuclear DNA contents. Annals of Botany 94 (2005) 255-260
16. Hacket J.D., Anderson D.M., Erdner D.L., and Bhattacharya D. Dinoflagellates: a remarkable evolutionary experiment. American Journal of Botany 91 10 (2004) 1523-1534
17. Huang B., Hou J., Lin S., Chen J., and Hong H. Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella. Harmful Algae 7 4 (2008) 495-503
18. Kremp A., and Heisaken A.S. Sexuality and cyst formation of the spring-bloom forming dinoflagellate Scrippsiella hangoei in the coastal northern Baltic Sea. Marine Biology 134 4 (1999) 771-777
19. Kremp A., and Anderson D.M. Factors regulation germination of calcified cysts of the spring bloom dinoflagellate Scrippsiella hangoei from the northern Baltic Sea. Journal of Plankton Research 22 7 (2000) 1311-1327
20. Kremp A., and Parrow M.W. Evidence for asexual resting cysts in the life cycle of the marine peridinoid dinoflagellate, Scrippsiella hangoei. Journal of Phycology 42 2 (2006) 400-409
21. Kremp A., Lindholm T., Dreßler N., Erler K., Gerdts G., Eirtovaara S., and Leskinen E. Blooming forming Alexandrium ostenfeldii (Dinophyceae) in shallow waters of the Åland Archipelago, Northern Baltic Sea. Harmful Algae 8 2 (2009) 318-328
22. LaJeunesse T.C., Lambert G., Andersen R.A., Coffroth M.A., and Galbraith D.W. Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates. Journal of Phycology 41 4 (2005) 880-886
23. Leitch I.J., and Bennet M.D. Genome downsizing in polyploidy plants. Biological Journal of the Linnean Society 82 (2004) 431-432
24. Lilly E.L., Halanych K.M., and Anderson D.M. Species boundaries and global biogeography of the Alexandrium tamarense complex (Dinophyceae). Journal of Phycology 43 6 (2007) 1329-1338
25. Matsuoka K., and Takeuchi T. Productivity of vegetative cells, planozygotes and calcified cysts of dinoflagellate Alexandrium catenella (Whedon et Kofoid) Balech based on the field observation. Fossils 59 (1995) 31-46
26. Ohno S. So much 'junk' in our genomes. In Evolution of Genetic Systems. In: Smith H.H. (Ed). Brookhaven Symposia in Biology (1972) 366-370
27. Parrow M.W., and Burkholder J.M. Flow cytometric determination of zoospore DNA content and population DNA distribution in cultured Pfiesteria spp. (Pyrrhophyta). Journal of Experimental Marine Biology and Ecology 267 1 (2002) 35-51
28. Parrow M.W., and Burkholder J.M. Estuarine heterotrophic cryptoperidiniopsoids (Dinophyceae): Life cycle and culture studies. Journal of Phycology 39 4 (2003) 678-696
29. Parrow M.W., Deamer N.J., Alexander J.L., and Burkholder J.M. A cell cycle synchronization and culture purification technique for heterotrophic Pfisteria and cryptoperidiniopsoid dinoflagellates analyzed by flow cytometry. In: Steidinger K.A., Landsberg J.H., Tomas C.R., and Vargo G.A. (Eds). Proceedings of the Xth International Conference on Harmful Algae (2004), Intergovernmental Oceanographic Commission of UNESCO, St. Petersburg, FL, USA 420-422
30. Rengefors K. Seasonal succession of dinoflagellates couled to the benthic cyst dynamics in Lake Erken. Archiv fuer Hydrobiologie (Special Issue) 51 (1998) 123-141
31. Rizzo P.J. Biochemistry of the dinoflagellate nucleus. In: Taylor F.J.R. (Ed). The Biology of Dinoflagellates (1987), Blackwell, Oxford 143-173
32. Robinson, J.P., Gregori, G., 2006. Principles of flow cytometry. In: Dolezel, Greilhuber, Suda (Eds.), Flow Cytometry with Plant Cells.
33. Swift H. The constancy of desoxyribose nucleic acid in plant nuclei. Proceedings of the National Academy of Sciences, USA 36 (1950) 643-654
34. Steidinger K., and Garcés E. Life cycles of harmful algae: an overview. In: Graneli E., and Turner J.T. (Eds). Ecology of Harmful Algae (2006), Springer Verlag 37-49
35. Taroncher-Oldenburg G., Kulis D.M., and Anderson D.M. Coupling of saxitoxin biosynthesis to the G1 phase of the cell cycle in the dinoflagellate Alexandrium fundyense: temperature and nutrient effects. Natural Toxins 7 (1999) 207-219
36. Van Dolah F., Leighfield T.A., Kamykowski D., and Kirkpatrick G.J. Cell cycle behaviour of laboratory and field populations of the Florida red tide dinoflagellate, Karenia brevis. Continental Shelf Research 28 1 (2008) 11-23
37. Veldhuis M.J.W., Cuccu T.L., and Sieracki M.E. Cellular DNA content of marine phytoplankton using two new fluorochromes: taxonomic and ecological implications. Journal of Phycology 33 3 (1997) 527-541
38. Von Dassow P., Petersen T.W., Chepurnov V.A., and Armbrust E.V. Inter- and intraspecific relationships between nuclear DNA content and cell size in selected members of the centric diatom genus Thalassiosira (Bacillariophyceae). Journal of Phycology 44 2 (2008) 335-349
39. Wang Z.-H., Qi Y.-Z., and Yang Y.-F. Cyst formation: an important mechanism for the termination of Scrippsiella trochoidea (Dinophyceae) bloom. Journal of Plankton Research 29 2 (2007) 209-218
40. Yoshimatsu S. Sexual reproduction of Protogonyaulax catenella in culture, 2. Determination of mating type. Bulletin of Plankton Society of Japan 31 (1984) 107-111