A validated model of GAG deposition, cell distribution, and growth of tissue engineered cartilage cultured in a rotating bioreactor

Biotechnology and Bioengineering

Volumn 105, Issue 4, 2010, Pages 842-853

  Nikolaev, N I ^a   Obradovic, B ^b   Versteeg, H K ^a   Lemon, G ^c   Williams, D J ^a  

^a LOUGHBOROUGH UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF BELGRADE   (Serbia)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Cartilage tissue engineering; Cell distribution; Glycosaminoglycan deposition; Mathematical model; Tissue growth

Indexed keywords

CARTILAGE TISSUE ENGINEERING; CELL DISTRIBUTION; GLYCOSAMINOGLYCAN DEPOSITION; GLYCOSAMINOGLYCANS; TISSUE GROWTH;

BIOREACTORS; CARTILAGE; CELLS; COMPUTER SIMULATION; CYTOLOGY; ELECTRIC DISCHARGE MACHINING; LIGAMENTS; MATHEMATICAL MODELS; OXYGEN; PHYSIOLOGICAL MODELS; ROTATION; TISSUE CULTURE;

TISSUE ENGINEERING;

GLYCOSAMINOGLYCAN; OXYGEN; PROTEOGLYCAN;

ARTICLE; BIOREACTOR; CARTILAGE; CELL COUNT; CELL DENSITY; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CORRELATION ANALYSIS; EXTRACELLULAR MATRIX; MATHEMATICAL MODEL; OXYGEN CONCENTRATION; OXYGEN CONSUMPTION; OXYGEN TRANSPORT; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; TISSUE CULTURE; TISSUE ENGINEERING; TISSUE GROWTH; TISSUE REGENERATION; ANIMAL; ARTICULAR CARTILAGE; BIOLOGICAL MODEL; CATTLE; CYTOLOGY; METABOLISM; METHODOLOGY; TIME; ULTRASTRUCTURE;

ANIMALS; BIOREACTORS; CARTILAGE, ARTICULAR; CATTLE; CELL PROLIFERATION; GLYCOSAMINOGLYCANS; MODELS, BIOLOGICAL; OXYGEN; TIME FACTORS; TISSUE ENGINEERING;

EID: 77449089927     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22581     Document Type: Article

References (32)

1. Bilgen B, Uygun K, Bueno EM, Sucosky P, Barabino GA. 2009. Tissue growth modeling in a wavy-walled bioreactor. Tissue Eng A 15(4):761-771.
2. Chung TJ. 2002. Computational fluid dynamics. Cambridge: Cambridge University Press.
3. Freed LE, Hollander AP, Martin I, Barry JR, Langer R, Vunjak-Novakovic G. 1998. Chondrogenesis in a cell-polymer-bioreactor system. Exp Cell Res 240(1):58-65.
4. Freshney RI, Obradovic B, Grayson WL, Cannizzaro C, Vunjak-Novakovic G. 2007. Principles of tissue culture and bioreactor design. In: Lanza RP, Langer R, Vacanti J, editors. Principles of tissue engineering. Burlington, San Diego, London: Academic Press. p 155-181.
5. Haselgrove JC, Shapiro IM, Silverton SF. 1993. Computer modeling of the oxygen supply and demand of cells of the avian growth cartilage. Am J Physiol 265(2):C497-C506.
6. Hasler EM, Herzog W, Wu JZ, Müller W, Wyss U. 1999. Articular cartilage biomechanics: Theoretical models, material properties, and biosynthetic response. Crit Rev Biomed Eng 27(6):415-488.
7. Himmelblau DM. 1964. Diffusion of dissolved gases in liquids. Chem Rev 64(5):527-550.
8. Hu JC, Athanasiou KA. 2006. The effects of intermittent hydrostatic pressure on self-assembled articular cartilage constructs. Tissue Eng 12(5):1337-1344.
9. Klein TJ, Sah RL. 2007. Modulation of depth-dependent properties in tissue-engineered cartilage with a semi-permeable membrane and perfusion: A continuum model of matrix metabolism and transport. Biomech Model Mechanobiol 6(1-2):21-32.
10. Lappa M. 2003. Organic tissues in rotating bioreactors: Fluid-mechanical aspects, dynamic growth models, and morphological evolution. Biotechnol Bioeng 84(5):518-532.
11. Lemon G, King JR. 2007. Multiphase modelling of cell behaviour on artificial scaffolds: Effects of nutrient depletion and spatially nonuniform porosity. Math Med Biol 24(1):57-83.
12. Martin I, Obradovic B, Freed LE, Vunjak-Novakovic G. 1999. Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage. Ann Biomed Eng 27(5): 656-662.
13. Mauck RL, Soltz MA, Wang CC, Wong DD, Chao PH, Valhmu WB, Hung CT, Ateshian GA. 2000. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng 122(3):252-260.
14. Mauck RL, Hung CT, Ateshian GA. 2003. Modeling of neutral solute transport in a dynamically loaded porous permeable gel: Implications for articular cartilage biosynthesis and tissue engineering. J Biomech Eng 125(3):602-614.
15. Moon AG, Tranquillo RT. 1993. Fibroblast-populated collagen microsphere assay of cell traction force: Part 1. Continuum model. AIChe J 39(1): 163-177.
16. Murray JD. 2003. Dermal wound healing. Mathematical biology II spatial models and biomedical applications. Berlin, Heidelberg: Springer Verlag.
17. Murray JD, Oster GF. 1984. Cell traction models for generating pattern and form in morphogenesis. J Math Biol 19(3):265-279.
18. Obradovic B, Carrier RL, Vunjak-Novakovic G, Freed LE. 1999. Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. Biotechnol Bioeng 63:197-205.
19. Obradovic B, Meldon JH, Freed LE, Vunjak-Novakovic G. 2000. Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. AIChe J 46(9):1860-1871.
20. Obradovic B, Radisic M, Vunjak-Novakovic G. 2007. Oxygen transport in tissue engineering systems: Cartilage and myocardium. FDMP 3(3): 189-202.
21. Pisu M, Lai N, Cincotti A, Concas A, Cao GC. 2004. Modeling of engineered cartilage growth in rotating bioreactors. Chem Eng Sci 59(22-23): 5035-5040.
22. Pisu M, Lai N, Concas A, Cao G. 2006. A novel simulation model for engineered cartilage growth in static systems. Tissue Eng 12(8):2311-2320.
23. Sengers BG, Please CP, Oreffo ROC. 2007a. Experimental characterization and computational modelling of two-dimensional cell spreading for skeletal regeneration. J R Soc Interface 4:1107-1117.
24. Sengers BG, Taylor M, Please CP, Oreffo ROC. 2007b. Computational modelling of cell spreading and tissue regeneration in porous scaffolds. Biomaterials 28(10):1926-1940.
25. Seog J, Dean D, Rolauffs B, Wu T, Genzer J, Plaas AHK, Grodzinsky AJ, Ortiz C. 2005. Nanomechanics of opposing glycosaminoglycan macromolecules. J Biomech 38(9):1789-1797.
26. Vunjak-Novakovic G, Goldstein SA. 2005. Biomechanical principles of cartilage and bone tissue engineering. In: Mow VC, Huiskes R, editors. Basic orthopaedic biomechanics and Mechanobiology. Philadelphia: Lippincott Williams and Wilkins. p 343-408.
27. Vunjak-Novakovic G, Freed LE, Biron RJ, Langer R. 1996. Effects of mixing on the composition and morphology of tissue-engineered cartilage. AIChE J 42(3):850-860.
28. Vunjak-Novakovic G, Obradovic B, Martin I, Bursac PM, Langer R, Freed LE. 1998. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol Prog 14(2):193-202.
29. Vunjak-Novakovic G, Martin I, Obradovic B, Treppo S, Grodzinsky AJ, Langer R, Freed LE. 1999. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J Orthop Res 17(1):130-138.
30. Vunjak-Novakovic G, Obradovic B, Martin I, Freed LE. 2002. Bioreactor studies of native and tissue engineered cartilage. Biorheology 39(1-2): 259-268.
31. Williamson AK, Chen AC, Sah RL. 2001. Compressive properties and function-composition relationships of developing bovine articular cartilage. J Orthop Res 19(6):1113-1121.
32. Wilson CG, Bonassar LJ, Kohles SS. 2002. Modeling the dynamic composition of engineered cartilage. Arch Biochem Biophys 408(2):246-254.