Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum

Journal of Bacteriology

Volumn 186, Issue 22, 2004, Pages 7645-7652

  Beckers, Gabriele ^a   Bendt, Anne K ^a,b   Krämer, Reinhard ^a   Burkovski, Andreas ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; CYTOPLASM PROTEIN; NITROGEN; REPRESSOR PROTEIN; UREA; UREASE;

ARTICLE; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; GENE CLUSTER; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENE IDENTIFICATION; GENETIC CODE; GENETIC TRANSCRIPTION; MOLECULAR GENETICS; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN TRANSPORT; REPRESSOR GENE;

ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; NITROGEN; OPERON; REPRESSOR PROTEINS; TRANSCRIPTION, GENETIC; UREA; UREASE;

ACTINOBACTERIA (CLASS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; UNCULTURED ACTINOMYCETE;

EID: 7744241359     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.22.7645-7652.2004     Document Type: Article

References (32)

1. Abe, S., K. Takayama, and S. Kinoshita. 1967. Taxonomical studies on glutamic acid-producing bacteria. J. Gen. Microbiol. 13:279-301.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. John Wiley and Sons, New York, N.Y.
3. Beckers, G., L. Nolden, and A. Burkovski. 2001. Glutamate synthase of Corynebacterium glutamicum is not essential for glutamate synthesis and is regulated by the nitrogen status. Microbiology 147:2961-2970.
4. Burkovski, A. 2003. I do it my way: regulation of ammonium uptake and ammonium assimilation in Corynebacterium glutamicum. Arch. Microbiol. 179:83-88.
5. Burkovski, A. 2003. Ammonium assimilation and nitrogen control in Corynebacterium glutamicum and its relatives: an example for new regulatory mechanisms in actinomycetes. FEMS Microbiol. Rev. 27:617-628.
6. Burkovski, A. Nitrogen metabolism and its regulation. In M. Bott, and L. Eggeling (ed.), Handbook of Corynebacterium glutamicum, in press. CRC Press LLC, Boca Raton, Fla.
7. Grant, S. N. G., J. Jessee, F. R. Bloom, and D. Hanahan. 1990. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. USA 87:4645-4649.
8. Hashimoto, J. G., A. S. Beadles-Bohling, and K. M. Wiren. 2004. Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis. BioTechniques 36:54-60.
9. Heller, K. B., E. C. Lin, and T. H. Wilson. 1980. Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J. Bacteriol. 144:274-278.
10. Hermann, T., G. Wersch, E.-M. Uhlemann, R. Schmid, and A. Burkovski. 1998. Mapping and identification of Corynebacterium glutamicum proteins by two-dimensional gel electrophoresis and microsequencing. Electrophoresis 19:3217-3221.
11. Hermann, T., M. Finkemeier, W. Pfefferte, G. Wersch, R. Krämer, and A. Burkovski. 2000. Two-dimensional electrophoretic analysis of Corynebacterium glutamicum membrane fraction and surface proteins. Electrophoresis 21:654-659.
12. Hermann, T., W. Pfefferte, C. Baumann, E. Busker, S. Schaffer, M. Bott, H. Sahm, N. Dusch, J. Kalinowski, A. Pühler, A. K. Bendt, R. Krämer, and A. Burkovski. 2001. Proteome analysis of Corynebacterium glutamicum. Electrophoresis 22:1712-1723.
13. Jahns, T., A. Zobel, D. Kleiner, and H. Kaltwasser. 1988. Evidence for carrier-mediated, energy-dependent uptake of urea in some bacteria. Arch. Microbiol. 149:377-383.
14. Jakoby, M., L. Nolden, J. Meier-Wagner, R. Krämer, and A. Burkovski. 2000. AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum. Mol. Microbiol. 37:964-977.
15. Kalinowski, J., B. Bathe, N. Bischoff, M. Bott, A. Burkovski, N. Dusch, L. Eggeling, B. J. Eikmanns, L. Gaigalat, A. Goesmann, M. Hartmann, K. Huthmacher, R. Krämer, B. Linke, A. C. McHardy, F. Meyer, B. Möckel, W. Pfefferle, A. Pühler, D. Rey, C. Rückert, H. Sahm, V. F. Wendisch, I. Wiegräbe, and A. Tauch. 2003. The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J. Biotechnol. 104:5-25.
16. Keilhauer, C., L. Eggeling, and H. Sahm. 1993. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175:5595-5603.
17. Krämer, R., and C. Lambert. 1990. Uptake of glutamate in Corynebacterium glutamicum. 2. Evidence for a primary active transport system. Eur. J. Biochem. 194:937-944.
18. Kronemeyer, W., N. Peekhaus, R. Krämer, L. Eggeling, and H. Sahm. 1995. Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum. J. Bacteriol. 177:1152-1158.
19. Mills, J., N. R. Wyborn, J. A. Greenwood, S. G. Williams, and C. W. Jones. 1998. Characterisation of a binding-protein-dependent, active transport system for short-chain amides and urea in the methylotrophic Methylophilus methylotrophus. Eur. J. Biochem. 251:45-53.
20. Mobley, H. L., M. D. Island, and R. P. Hausinger. 1995. Molecular biology of microbial ureases. Microbiol. Rev. 59:451-480.
21. Nolden, L., G. Beckers, B. Möckel, W. Pfefferle, K. M. Nampoothiri, R. Krämer, and A. Burkovski. 2000. Urease of Corynebacterium glutamicum: organization of corresponding genes and investigation of activity. FEMS Microbiol. Lett. 189:305-310.
22. Nolden, L., M. Farwick, R. Krämer, and A. Burkovski. 2001. Glutamine synthetases in Corynebacterium glutamicum: transcriptional control and regulation of activity. FEMS Microbiol. Lett. 201:91-98.
23. Poore, C. A., and H. L. T. Mobley. 2003. Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS. Microbiology 149:3383-3394.
24. Puskás, L. G., M. Inui, and H. Yukawa. 2000. Structure of the urease operon of Corynebacterium glutamicum. DNA Seq. 11:383-394.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Sebbane, F., S. Bury-Moné, K. Cailliau, E. Browaeys-Poly, H. De Reuse, and M. Simonet. 2002. The Yersinia pseudotuberculosis Yut protein, a new type of urea transporter homologous to eukaryotic channels and functionally interchangeable in vitro with the Helicobacter pylori UreI protein. Mol. Microbiol. 45:1165-1174.
27. Siewe, R. M., B. Weil, A. Burkovski, L. Eggeling, R. Krämer, and T. Jahns. 1998. Urea uptake and urease activity in Corynebacterium glutamicum. Arch. Microbiol. 169:411-416.
28. Tauch, A., O. Kirchner, B. Löffler, S. Götker, A. Pühler, and J. Kalinowski. 2002. Efficient electrotransformation of Corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1. Curr. Microbiol. 45:362-367.
29. Valladares, A., M. L. Montesinos, A. Herrero, and E. Flores. 2002. An ABC-type, high-affinity urea permease identified in cyanobacteria. Mol. Microbiol. 43:703-715.
30. +-gated urea channel: the link between Helicobacter pylori urease and gastric colonization. Science 287:482-485.
31. Wray, L. V., Jr., A. E. Ferson, and S. H. Fisher. 1997. Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H. J. Bacteriol. 179:5494-5501.
32. Yanisch-Perron, C., L. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mp18 and pUC19 vectors. Gene 33:103-119.