Reflections on protein splicing: Structures, functions and mechanisms

Proceedings of the Japan Academy Series B: Physical and Biological Sciences

Volumn 85, Issue 9, 2009, Pages 409-421

  Anraku, Yasuhiro ^a   Satow, Yoshinori ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Extein; Intein; Protein splicing; Thiazolidine intermediate; VDE; VMA1

Indexed keywords

EXTEIN; INTEIN; PEPTIDE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; SACCHAROMYCES CEREVISIAE PROTEIN; TFP1 PROTEIN, S CEREVISIAE; THIAZOLIDINE DERIVATIVE;

CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN PROCESSING; REVIEW;

EXTEINS; INTEINS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN SPLICING; PROTON-TRANSLOCATING ATPASES; SACCHAROMYCES CEREVISIAE PROTEINS; THIAZOLIDINES;

SACCHAROMYCES CEREVISIAE;

EID: 77349101326     PISSN: 03862208     EISSN: 13492896     Source Type: Journal    
DOI: 10.2183/pjab.85.409     Document Type: Review

References (34)

1. +-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 265, 6726-6733.
2. Gimble, F. S. and Thorner, J. (1992) Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature 357, 301-306.
3. +-adenosine triphosphatase. Science 250, 651-657.
4. Perler, F. B. (2002) In Base, the intein database. Nucl. Acids Res. 30, 383-384. Web site at http://www.neb.com/neb/inteins.html, New England Biolabs, Beverly, MA, USA.
5. Kawasaki, M., Makino, S., Matsuzawa, H., Satow, Y., Ohya, Y. and Anraku, Y. (1996) Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme. Biochem. Biophys. Res. Commun. 222, 827-832.
6. Kawasaki, M., Nogami, S., Satow, Y., Ohya, Y. and Anraku, Y. (1997) Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme: A random mutagenesis study of the entire VMA1-derived endonuclease sequence. J. Biol. Chem. 272, 15668-15674.
7. Kawasaki, M., Satow, Y., Ohya, Y. and Anraku, Y. (1997) Protein splicing in the yeast Vma1 protozyme: Evidence for an intramolecular reaction. FEBS Lett. 412, 518-520.
8. Nogami, S., Satow, Y., Ohya, Y. and Anraku, Y. (1997) Probing novel elements for protein splicing in the yeast Vma1 protozyme: a study of replacement mutagenesis and intragenic suppression. Genetics 147, 73-85.
9. Chong, S., Shao, Y., Paulus, H., Benner, J., Perler, F. B. and Xu, M. Q. (1996) Protein splicing involving the Saccharomyces cerevisiae VMA intein: The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J. Biol. Chem. 271, 22159-22168.
10. Chong, S., Williams, K. S, Wotkowicz, C. and Xu, M. Q. (1998) Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J. Biol. Chem. 273, 10567-10577.
11. Paulus, H. (1998) The chemical basis of protein splicing. Chem. Soc. Rev. 27, 375-386.
12. Davis, E. O., Jenner, P. J., Brooks, P. C., Colston, M. J. and Sedgwick, S. G. (1992) Protein splicing in the maturation of M. tuberculosis recA protein. A mechanism for tolerating a novel class of intervening sequence. Cell 71, 201-210.
13. Xu, M-Q., Southworth, M. W., Mersha, F. B., Hornstra, L. J. and Perler, F. G. (1993) In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 75, 1371-1377.
14. Cooper, A. A., Chen, Y.-J., Lindorfer, M. A. and Stevens, T. H. (1993) Protein splicing of the yeast TFP1 intervening protein sequence: A model for self-excision. EMBO J. 12, 2575-2583.
15. Shingledecker, K., Jiang, S-Q. and Paulus, H. (1998) Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments. Gene 207, 187-195.
16. Anraku, Y. (1997) Protein splicing: Its chemistry and biology. Genes to Cells 2, 359-367.
17. Noren, C. J., Wang, J. and Perler, F. B. (2000) Dissecting the chemistry of protein splicing and its applications. Angew. Chem. Int. Ed. 39, 450-466.
18. Paulus, H. (2000) Protein splicing and related forms of protein autoprocessing. Annu. Rev. Biochem. 69, 447-496.
19. Mizutani, R., Satow, Y., Ohya, Y. and Anraku, Y. (1999) Crystal structure of the VMA1-derived endonuclease bearing an N-terminal propeptide. Acta Crystallogr. A 55 (Suppl.), P06.04.031.
20. Mizutani, R., Nogami, S., Kawasaki, M., Ohya, Y., Anraku, Y. and Satow, Y. (2002) Protein-splicing reaction via a thiazolidine intermediate: Crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides. J. Mol. Biol. 316, 919-929.
21. Mizutani, R., Anraku, Y. and Satow, R. (2004) Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates. J. Synchrotron Rad. 11, 109-112.
22. 1-ATPases. J. Biol. Chem. 263, 9102-9112.
23. Bowman, E. J., Tenney, K. and Bowman, B. J. (1988) Isolation of genes encoding the Neurospora vacuolar ATPase: Analysis of VMA-1 encoding the 67-kDa subunit reveals homology to other ATPases. J. Biol. Chem. 263, 13994-14001.
24. +-ATPase, inhibit its processing by protein splicing. Biochem. Biophys. Res. Commun. 188. 40-47.
25. Pietrokovski, S. (1994) Conserved sequence features of inteins (protein introns) and their use in identifying new inteins and related proteins. Protein Sci. 3, 2340-2350.
26. Anraku, Y., Mizutani, R. and Satow, Y. (2005) Protein splicing: Its discovery and structural insight into novel chemical mechanisms. IUBMB Life 57, 563-574.
27. Duan, X., Gimble, F. S. and Quicho, F. A. (1997) Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell 89, 555-564.
28. Poland, B. W., Xu, M. Q. and Quicho, F. A. (2000) Structural insights into the protein splicing mechanism of PI-SceI. J. Biol. Chem. 275, 16408-16413.
29. Mills, K. V. and Paulus, H. (2001) Reversible inhibition of protein splicing by zinc ion. J. Biol. Chem. 276, 10832-10838.
30. Klabunde, T., Sharma, S., Telenti, A., Jacobs, W. R. and Sacchettini, J. C. (1998) Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nature Struct. Biol. 5, 31-36.
31. Hall, T. M., Porter, J. A., Young, K. E., Koonin, E. V., Beachy, P. A. and Leahy, D. J. (1997) Crystal structure of a Hedgehog autoprocessing domain: homology between Hedgehog and self-splicing proteins. Cell 91, 85-97.
32. Romanelli, A., Shekhtman, A., Cowburn, D. and Muir, T. W. (2004) Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. Proc. Natl. Acad. Sci. USA 101, 6397-6402.
33. 4S. Acta Crystallogr. C 40, 2050-2053.
34. Ludwig, C., Schwarzer, D. and Mootz, H. D. (2008) Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J. Bio. Chem. 283, 25264-25272.