Purification of a defensin isolated from Vigna unguiculata seeds, its functional expression in Escherichia coli, and assessment of its insect α-amylase inhibitory activity

Protein Expression and Purification

Volumn 71, Issue 1, 2010, Pages 8-15

  dos Santos, Izabela S ^a   Carvalho, André de O ^a   de Souza Filho, Gonçalo A ^a   do Nascimento, Viviane V ^a   Machado, Olga L T ^a   Gomes, Valdirene M ^a  

^a UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO   (Brazil)

Author keywords

Amylase inhibitor; Cowpea seeds; Functional expression; Plant defensin

Indexed keywords

AMYLASE; DEFENSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOCHEMISTRY; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LEGUME; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; WEEVIL;

ALPHA-AMYLASES; AMINO ACID SEQUENCE; ANIMALS; BIOCHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; DEFENSINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ASSAYS; ESCHERICHIA COLI; FABACEAE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; SEEDS; SEQUENCE ANALYSIS, PROTEIN; WEEVILS;

CALLOSOBRUCHUS MACULATUS; CURCULIONIDAE; ESCHERICHIA COLI; HEXAPODA; MAMMALIA; VIGNA UNGUICULATA; ZABROTES SUBFASCIATUS;

EID: 77049104040     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.11.008     Document Type: Article

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