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Volumn 51, Issue 13, 2010, Pages 1803-1805

Cytosporones O, P and Q from an endophytic Cytospora sp.

Author keywords

Cytospora; Cytosporones; Dothiorelones; Fungal endophytes; Phomopsis

Indexed keywords

ANTIBIOTIC AGENT; CYTOSPORONE B; CYTOSPORONE C; CYTOSPORONE D; CYTOSPORONE E; CYTOSPORONE O; CYTOSPORONE P; CYTOSPORONE Q; DOTHIORELONE A; DOTHIORELONE B; DOTHIORELONE C; UNCLASSIFIED DRUG;

EID: 76949103410     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2010.01.110     Document Type: Article
Times cited : (28)

References (17)
  • 5
    • 76949092317 scopus 로고    scopus 로고
    • CML-Mycological Collection of Lavras, Federal University of Lavras, Brazil; IBT-IBT Culture Collection, Center for Microbial Biotechnology, Technical University of Denmark
    • CML-Mycological Collection of Lavras, Federal University of Lavras, Brazil; IBT-IBT Culture Collection, Center for Microbial Biotechnology, Technical University of Denmark.
  • 7
    • 76949092205 scopus 로고    scopus 로고
    • note
    • Malt Extract Agar (MEA)-malt extract (Difco) 20 g, peptone (Difco) 1 g, glucose (BHD) 20 g, distilled water 750 mL.
  • 8
    • 76949093416 scopus 로고    scopus 로고
    • note
    • 2O-MeOH 90:10, 3 fractions; 90:10-0:100, 9 fractions; 0:100, 4 fractions) to give 16 fractions. Fractions 09 (316 mg), 10 (281 mg), and 11 (440 mg) were individually submitted to step-wise normal phase flash chromatography over 10 g diol cartridges (Biotage) eluting from 100% heptane to 100% dichloromethane in 50% steps, and from dichloromethane to 100% ethyl acetate in 10% steps, with two additional elutions with ethyl acetate-methanol (90:10 and 80:20) and a final wash with 100% methanol to afford another 16 sub fractions each (09_01 to 09_16, 10_01 to 10_16, and 11_01 to 11_16). All solvents were HPLC grade and were purchased from Merck.
  • 9
    • 76949091124 scopus 로고    scopus 로고
    • note
    • Analytical HPLC was performed on a HP 1100 system equipped with a diode array detector (Agilent) using a Luna II C18 column (100 × 2 mm, 3 μm; Phenomenex) and an elution gradient of water-acetonitrile from 85:15 to 0:100 in 20 min plus 5 min of pure acetonitrile; both solvents containing 50 ppm of trifluoroacetic acid.
  • 11
    • 76949088462 scopus 로고    scopus 로고
    • note
    • 2O-ACN 68:32-66:34 in 12 min, plus 5 min of 100% ACN) to afford dothiorelones A-C and H (2), and compounds 1, 3, and 4.
  • 12
    • 76949096363 scopus 로고    scopus 로고
    • note
    • 13C and 2D NMR spectra for compounds 1, 3, and 4 were acquired in Bruker Avance 800 MHz using standard pulse sequences.
  • 17
    • 76949095961 scopus 로고    scopus 로고
    • note
    • 20 -52.1 (c 0.001, MeOH).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.