-
4
-
-
29344444077
-
-
Larsen T.O., Smedsgaard J., Nielsen K.F., Hansen M.E., and Frisvad J.C. Nat. Prod. Rep. 22 (2005) 672
-
(2005)
Nat. Prod. Rep.
, vol.22
, pp. 672
-
-
Larsen, T.O.1
Smedsgaard, J.2
Nielsen, K.F.3
Hansen, M.E.4
Frisvad, J.C.5
-
5
-
-
76949092317
-
-
CML-Mycological Collection of Lavras, Federal University of Lavras, Brazil; IBT-IBT Culture Collection, Center for Microbial Biotechnology, Technical University of Denmark
-
CML-Mycological Collection of Lavras, Federal University of Lavras, Brazil; IBT-IBT Culture Collection, Center for Microbial Biotechnology, Technical University of Denmark.
-
-
-
-
7
-
-
76949092205
-
-
note
-
Malt Extract Agar (MEA)-malt extract (Difco) 20 g, peptone (Difco) 1 g, glucose (BHD) 20 g, distilled water 750 mL.
-
-
-
-
8
-
-
76949093416
-
-
note
-
2O-MeOH 90:10, 3 fractions; 90:10-0:100, 9 fractions; 0:100, 4 fractions) to give 16 fractions. Fractions 09 (316 mg), 10 (281 mg), and 11 (440 mg) were individually submitted to step-wise normal phase flash chromatography over 10 g diol cartridges (Biotage) eluting from 100% heptane to 100% dichloromethane in 50% steps, and from dichloromethane to 100% ethyl acetate in 10% steps, with two additional elutions with ethyl acetate-methanol (90:10 and 80:20) and a final wash with 100% methanol to afford another 16 sub fractions each (09_01 to 09_16, 10_01 to 10_16, and 11_01 to 11_16). All solvents were HPLC grade and were purchased from Merck.
-
-
-
-
9
-
-
76949091124
-
-
note
-
Analytical HPLC was performed on a HP 1100 system equipped with a diode array detector (Agilent) using a Luna II C18 column (100 × 2 mm, 3 μm; Phenomenex) and an elution gradient of water-acetonitrile from 85:15 to 0:100 in 20 min plus 5 min of pure acetonitrile; both solvents containing 50 ppm of trifluoroacetic acid.
-
-
-
-
11
-
-
76949088462
-
-
note
-
2O-ACN 68:32-66:34 in 12 min, plus 5 min of 100% ACN) to afford dothiorelones A-C and H (2), and compounds 1, 3, and 4.
-
-
-
-
12
-
-
76949096363
-
-
note
-
13C and 2D NMR spectra for compounds 1, 3, and 4 were acquired in Bruker Avance 800 MHz using standard pulse sequences.
-
-
-
-
13
-
-
0034649733
-
-
Brady S.F., Wagenaar M.M., Singh M.P., Janso J.E., and Clardy J. Org. Lett. 2 (2000) 4043
-
(2000)
Org. Lett.
, vol.2
, pp. 4043
-
-
Brady, S.F.1
Wagenaar, M.M.2
Singh, M.P.3
Janso, J.E.4
Clardy, J.5
-
14
-
-
8644242110
-
-
Xu Q., Wang J., Huang Y., Zheng Z., Song S., Zhang Y., and Su W. Acta Oceanol. Sin. 23 (2004) 541
-
(2004)
Acta Oceanol. Sin.
, vol.23
, pp. 541
-
-
Xu, Q.1
Wang, J.2
Huang, Y.3
Zheng, Z.4
Song, S.5
Zhang, Y.6
Su, W.7
-
15
-
-
76949085199
-
-
Shen Y., Du X., Zheng Z., Huang Y., Song S., and Su W. Faming Zhuanli Shenqing Gongkai Shuomingshu (2006) 11
-
(2006)
Faming Zhuanli Shenqing Gongkai Shuomingshu
, pp. 11
-
-
Shen, Y.1
Du, X.2
Zheng, Z.3
Huang, Y.4
Song, S.5
Su, W.6
-
16
-
-
71749090849
-
-
Xu J., Kjer J., Sendker J., Wray V., Guan H., Edrada R., Müller W.E.G., Bayer M., Lin W., Wu J., and Proksch P. Bioorg. Med. Chem. 17 (2009) 7362
-
(2009)
Bioorg. Med. Chem.
, vol.17
, pp. 7362
-
-
Xu, J.1
Kjer, J.2
Sendker, J.3
Wray, V.4
Guan, H.5
Edrada, R.6
Müller, W.E.G.7
Bayer, M.8
Lin, W.9
Wu, J.10
Proksch, P.11
-
17
-
-
76949095961
-
-
note
-
20 -52.1 (c 0.001, MeOH).
-
-
-
|