Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease

Protein Engineering, Design and Selection

Volumn 23, Issue 3, 2010, Pages 147-152

  Ogino, Hiroyasu ^a   Tsuchiyama, Shotaro ^a   Yasuda, Masahiro ^a   Doukyu, Noriyuki ^b  

^a Osaka City University   (Japan)

^b TOYO UNIVERSITY   (Japan)

Author keywords

Aspartame precursor; Peptide synthesis; Protease; Reverse reaction; Thermolysin

Indexed keywords

ACTIVE CENTER; INDUSTRIAL CATALYST; PEPTIDE SYNTHESIS; REACTION YIELDS; REVERSE REACTIONS; STRUCTURAL COMPARISON; SYNTHESIS RATE; THERMOLYSIN;

AMINO ACIDS; CATALYSTS; ENZYMES; ESTERS; ORGANIC ACIDS; ORGANIC SOLVENTS; PEPTIDES; PHOSPHATASES;

SYNTHESIS (CHEMICAL);

AMINO ACID; ARGININE; ASPARTAME; ORGANIC SOLVENT; PHENYLALANINE; PROTEINASE; PST 01 PROTEASE; SERINE; THERMOLYSIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYST; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SYNTHESIS; MUTAGENESIS; MUTATION; NONHUMAN; PRECURSOR; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ASPARTAME; BINDING SITES; ENZYME STABILITY; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; ORGANIC CHEMICALS; PEPTIDE HYDROLASES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SOLVENTS; SUBSTRATE SPECIFICITY; THERMOLYSIN;

EID: 76949097506     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp086     Document Type: Article

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