Cell-free production of functional antibody fragments

Bioprocess and Biosystems Engineering

Volumn 33, Issue 1, 2010, Pages 127-132

  Oh, In Seok ^a   Lee, Ji Chul ^b   Lee, Myung Shin ^c   Chung, Jun Ho ^d   Kim, Dong Myung ^e  

^a Seoul National University   (South Korea)

^b Abxign Inc   (South Korea)

^c Eulji University   (South Korea)

^d Seoul National University College of Medicine   (South Korea)

^e Chungnam National University   (South Korea)

Author keywords

Antibody; Antigen binding fragment (Fab); Botulinum neurotoxin; Cell free protein synthesis system; Disulfide bond

Indexed keywords

ANIMAL STUDIES; ANTIBODY FRAGMENT; ANTIBODY-ANTIGEN; BOTULINUM NEUROTOXINS; CELL EXTRACTS; CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE PRODUCTION; DISULFIDE BONDS; E. COLI; FUNCTIONAL ANTIBODY FRAGMENTS; ISOMERASES; MOLECULAR CHAPERONES; OPTIMIZED REACTION CONDITIONS; REACTION MIXTURE; REDOX CONDITION; REDOX POTENTIALS; REDUCING ACTIVITY; SEROTYPES;

ANTIGENS; ESCHERICHIA COLI; GENE EXPRESSION; SYNTHESIS (CHEMICAL);

ANTIBODIES;

BOTULINUM TOXIN B; CELL EXTRACT; CHAPERONE; DISULFIDE; IMMUNOGLOBULIN F(AB) FRAGMENT; ISOMERASE; NEUTRALIZING ANTIBODY;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; BINDING AFFINITY; CELL FREE SYSTEM; DISULFIDE BOND; ESCHERICHIA COLI; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ANTIBODIES, MONOCLONAL; BOTULINUM TOXINS; CELL-FREE SYSTEM; ESCHERICHIA COLI; IMMUNOGLOBULIN FAB FRAGMENTS; RECOMBINANT PROTEINS;

ANIMALIA;

EID: 76849108413     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-009-0372-3     Document Type: Article

References (24)

1. HE Chadd SM Chamow 2001 Therapeutic antibody expression technology Curr Opin Biotechnol 12 188 194 10.1016/S0958-1669(00)00198-1 1:CAS:528: DC%2BD3MXjt1Olu7c%3D (Pubitemid 32247407)
2. J Krauss 2003 Recombinant antibodies for the diagnosis and treatment of cancer Mol Biotechnol 25 1 17 10.1385/MB:25:1:1 1:CAS:528:DC%2BD3sXnt1Wgtbo%3D (Pubitemid 37100233)
3. P Holliger PJ Hudson 2005 Engineered antibody fragments and the rise of single domains Nat Biotechnol 23 1126 1136 10.1038/nbt1142 1:CAS:528: DC%2BD2MXpvVyrtrc%3D (Pubitemid 41486394)
4. HJ de Haard N van Neer A Reurs SE Hufton RC Roovers P Henderikx AP de Bruine JW Arends HR Hoogenboom 1999 A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies J Biol Chem 274 18218 18230 10.1074/jbc.274.26.18218 (Pubitemid 29293808)
5. LG Presta 2002 Engineering antibodies for therapy Curr Pharm Biotechnol 3 237 256 10.2174/1389201023378256 1:CAS:528:DC%2BD38Xlslyjurs%3D (Pubitemid 34826556)
6. PJ Carter 2006 Potent antibody therapeutics by design Nat Rev Immunol 6 343 357 10.1038/nri1837 1:CAS:528:DC%2BD28XjslOhu7o%3D
7. SM Kipriyanov F Le Gall 2004 Generation and production of engineered antibodies Mol Biotechnol 26 39 60 10.1385/MB:26:1:39 1:CAS:528: DC%2BD2cXhtFSmsLc%3D (Pubitemid 38174858)
8. WA Marasco J Sui 2007 The growth and potential of human antiviral monoclonal antibody therapeutics Nat Biotechnol 25 1421 1434 10.1038/nbt1363 1:CAS:528:DC%2BD2sXhsVSjurvF (Pubitemid 350233138)
9. DP Humphreys 2003 Production of antibodies and antibody fragments in Escherichia coli and a comparison of their functions, uses and modification Curr Opin Drug Discov Devel 6 188 196 1:CAS:528:DC%2BD3sXislOqsL0%3D
10. HR Hoogenboom 2005 Selecting and screening recombinant antibody libraries Nat Biotechnol 23 1105 1116 10.1038/nbt1126 1:CAS:528:DC%2BD2MXpvVyrsb0%3D (Pubitemid 41486392)
11. DM Kim CY Choi JH Ahn TW Kim NY Kim IS Oh CG Park 2006 Development of a rapid and productive cell-free protein synthesis system Biotechnol Bioprocess Eng 11 235 239 10.1007/BF02932036 1:CAS:528:DC%2BD28XmvVCgs7k%3D
12. TW Kim IS Oh JW Keum YC Kwon JY Byun KH Lee CY Choi DM Kim 2007 Prolonged cell-free protein synthesis using dual energy sources: combined use of creatine phosphate and glucose for the efficient supply of ATP and retarded accumulation of phosphate Biotechnol Bioeng 97 1510 1515 10.1002/bit.21337 1:CAS:528:DC%2BD2sXotleqsb4%3D (Pubitemid 47204029)
13. DM Kim JR Swartz 2004 Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli Biotechnol Bioeng 85 122 129 10.1002/bit.10865 1:CAS:528:DC%2BD2cXitleltQ%3D%3D (Pubitemid 38141113)
14. LA Ryabova D Desplancq AS Spirin A Pluckthun 1997 Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones Nat Biotechnol 15 79 84 10.1038/nbt0197-79 1:CAS:528: DyaK2sXislOjsg%3D%3D
15. X Jiang Y Ookubo I Fujii H Nakano T Yamane 2002 Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system FEBS Lett 514 290 294 10.1016/S0014-5793(02) 02383-9 1:CAS:528:DC%2BD38Xis1ejtLc%3D (Pubitemid 34273956)
16. MS Lee JC Lee CY Choi J Chung 2008 Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display Hybridoma 27 18 24 10.1089/hyb.2007.0532 1:CAS:528:DC%2BD1cXitlekt70%3D (Pubitemid 351301376)
17. DM Gill 1982 Bacterial toxins: a table of lethal amounts Microbiol Rev 46 86 94 1:CAS:528:DyaL38XhsFyrtr4%3D
18. H Kadokura F Katzen J Beckwith 2003 Protein disulfide bond formation in prokaryotes Annu Rev Biochem 72 111 135 10.1146/annurev.biochem.72.121801.161459 1:CAS:528:DC%2BD3sXntFSgtL8%3D (Pubitemid 36930443)
19. DM Kim T Kigawa CY Choi S Yokoyama 1996 A highly efficient cell-free, protein synthesis system from Escherichia coli Eur J Biochem 239 881 886 10.1111/j.1432-1033.1996.0881u.x 1:CAS:528:DyaK28XltFaqs7g%3D
20. SH Kang DM Kim HJ Kim SY Jun KY Lee 2005 Cell-free production of aggregation-prone proteins in soluble and active forms Biotechnol Prog 21 1412 1419 10.1021/bp050087y 1:CAS:528:DC%2BD2MXmvFCnurc%3D (Pubitemid 41442997)
21. J Chung C Rader M Popkov YM Hur HK Kim YJ Lee CF Barbas 3rd 2004 Integrin alphaIIbbeta3-specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation FASEB J 18 361 363 10.1096/fj.04-2126com 1:CAS:528:DC%2BD2cXht1Gjt7o%3D (Pubitemid 39463706)
22. IS Oh DM Kim TW Kim CG Park CY Choi 2006 Providing an oxidizing environment for the cell-free expression of disulfide-containing proteins by exhausting the reducing activity of Escherichia coli S30 extract Biotechnol Prog 22 1225 1228 10.1021/bp060051l 1:CAS:528:DC%2BD28XmtF2ru7g%3D (Pubitemid 44230715)
23. DM Kim CY Choi 1996 A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane Biotechnol Prog 12 645 649 10.1021/bp960052l 1:CAS:528:DyaK28XlsFCksLk%3D (Pubitemid 26368069)
24. DD Buechter KF Medzihradszky AL Burlingame GL Kenyon 1992 The active site of creatine kinase. Affinity labeling of cysteine 282 with N-(2, 3-epoxypropyl)-N-amidinoglycine J Biol Chem 267 2173 2178 1:CAS:528: DyaK38XhtVWktb4%3D